| D Hopkins Plumbing & Heating Limited | Construction | 2016 |
| D Yeates Contract Cleaning Ltd | Administrative & support | 2003 |
| D&D Hospitality Group Limited | Hospitality | 2025 |
| Dalmar International Limited | Transport & logistics | 1997 |
| Danielle Grover Custom Furniture & Carpentry Ltd | Manufacturing | 2024 |
| Danmak Care Ltd | Wholesale & retail | 2019 |
| Darwish Property Limited | Real estate | 2021 |
| Davencroft Innovation Services Limited | Extraterritorial | 2020 |
| Davencroft IP (2) Limited | Extraterritorial | 2025 |
| Davencroft Limited | Wholesale & retail | 1999 |
| Dean's Kitchen Limited | Transport & logistics | 1985 |
| Deckflat Limited | Real estate | 1997 |
| Deefa Demolition Ltd | Construction | 2025 |
| Deena PA Ltd | Administrative & support | 2025 |
| Denton Island Garage Ltd. | Wholesale & retail | 2001 |
| Develop Outdoors CIC | Education | 2017 |
| Develop Outdoors Trading Ltd | Construction | 2026 |
| Dial for Disposal Ltd | Water & waste | 2025 |
| DJ Fencing & Landscaping Ltd | Administrative & support | 2025 |
| Donfily DRY Lining Ltd | Construction | 2012 |
| Douglas Consultants Ltd | Professional & scientific | 2025 |
| Downey Fishing Company Ltd | Agriculture & fishing | 2015 |
| Downs Send Consultancy Ltd | Education | 2021 |
| Drip N WET Ltd | Construction | 2015 |
| DTF Consulting (Worthing) Limited | Professional & scientific | 2011 |
| Duke & Hyde Limited | Other services | 2016 |
| DYL Services Ltd | Water & waste | 2023 |
| Dynamic Property Management Ltd | Real estate | 2003 |
| Dynamo Logistic Ltd | Transport & logistics | 2021 |
| E Plan Management Company Limited | Administrative & support | 1997 |
| E Plan Solutions Limited | Manufacturing | 2007 |
| Earith Limited | Manufacturing | 2026 |
| East Quay Estates Limited | Construction | 2017 |
| East Sussex Children's Allergy Clinic Ltd | Health & social care | 2026 |
| East Sussex Forest School Nursery Ltd | Education | 2013 |
| Easy Clean Sussex Ltd | Administrative & support | 2023 |
| Easy Host (Sussex) Ltd | Real estate | 2022 |
| Easytone Ltd | Arts & recreation | 2015 |
| Eating OUR Way to Extinction Limited | Information & communication | 2016 |
| Eco-Apt Ltd | Professional & scientific | 2024 |
| EGG Electrical Services Ltd | Manufacturing | 2025 |
| Eiger Group Sussex Ltd | Construction | 2019 |
| Elise Care & Aesthetics Ltd | Administrative & support | 2025 |
| Elite Grow Ltd | Professional & scientific | 2024 |
| Elite Industries Ltd | Construction | 2011 |
| Elite-Msg Systems & Security Ltd | Administrative & support | 2011 |
| Elmeridge Cable Solutions Limited | Extraterritorial | 2013 |
| Elmeridge Cables Limited | Manufacturing | 1987 |
| Ending Capitalism Ltd | Financial services | 2008 |
| Equipment Suppliers Ltd Limited | Manufacturing | 2023 |
| Eric Stuart Limited | Extraterritorial | 2018 |
| ESS Jones Ltd | Transport & logistics | 2021 |
| Essex Assistance Ltd | Health & social care | 2024 |
| Essex Mews RTM Company Limited | Households | 2007 |
| EVA DI Milo Holdings Limited | Administrative & support | 2021 |
| Every Expression Therapies Ltd | Health & social care | 2024 |
| Experience IT NOW Limited | Administrative & support | 2015 |
| F.& C.Automatic Production Limited | Manufacturing | 1962 |
| Fairlight Coachworks Limited | Wholesale & retail | 2010 |
| Fairlight Residents Company Limited | Households | 2011 |
| Faithful Court RTM Company Limited | Households | 2023 |
| Faithful Management Limited | Real estate | 2025 |
| Falcone Baking Company Limited | Manufacturing | 2006 |
| FAR Afield Ltd | Manufacturing | 2012 |
| Feeling-Inspired Limited | Professional & scientific | 2015 |
| Feilo Sylvania Europe Limited | Manufacturing | 1992 |
| Feilo Sylvania Fixtures UK Limited | Manufacturing | 1950 |
| Feilo Sylvania UK Limited | Wholesale & retail | 1950 |
| Field Food Ltd | Hospitality | 2023 |
| Fine Marketing Consultants Ltd | Information & communication | 2018 |
| First 4 Logistics Limited | Transport & logistics | 2018 |
| First 4 Sameday Ltd | Transport & logistics | 2021 |
| Fitzroy Travel Ltd | Administrative & support | 2017 |
| Fitzwilliam Language Services (FLS) Limited | Education | 2008 |
| Five Fingers (Holdings) Limited | Professional & scientific | 2009 |
| FIXINGS4U Ltd | Wholesale & retail | 2025 |
| Flamingo Works Ltd | Manufacturing | 2010 |
| Flory Corporate Secretaries Limited | Professional & scientific | 2013 |
| Fludes (Carpets) Limited | Wholesale & retail | 1952 |
| Flying Chef Catering Ltd | Hospitality | 2019 |
| Foligni's Ltd | Hospitality | 2021 |
| Forrest Boxing Limited | Arts & recreation | 2024 |
| Fort Products Limited | Wholesale & retail | 2005 |
| Fort Rise Freeholders Limited | Households | 2009 |
| Fowler & Co (Brighton) Limited | Manufacturing | 2012 |
| FOX Grange Property Ltd | Real estate | 2025 |
| Foyne Jones Recruitment Group Ltd | Professional & scientific | 2017 |
| Friars Court (Peacehaven) RTM Company Limited | Households | 2020 |
| Fuller Plumbing and Heating Limited | Construction | 2020 |
| Fusi & Tunskyy Ltd | Hospitality | 2026 |
| G W Ironworks Ltd | Manufacturing | 2003 |
| Galaxy Commercial Investment Ltd | Real estate | 2020 |
| Galaxy Commercial Ltd | Wholesale & retail | 2015 |
| Gamebound Limited | Information & communication | 2024 |
| Ganapati Transport Limited | Administrative & support | 2022 |
| Gateway M2M Limited | Information & communication | 2014 |
| GDC Building Services Ltd | Construction | 2005 |
| Geering Electrical Ltd | Construction | 2019 |
| Genesis Film Production Ltd | Information & communication | 2017 |
| Gilded Portraits Limited | Arts & recreation | 2026 |