| Eco Sales & Services Ltd | Wholesale & retail | 2016 |
| Ecodomus Developments Ltd | Construction | 2024 |
| ECOGRANTS4WALES Ltd | Health & social care | 2022 |
| Ecotech Green Cleaning Solutions Ltd | Administrative & support | 2025 |
| Ecstatic Disaster Ltd | Professional & scientific | 2021 |
| Edathamara Limited | Administrative & support | 2016 |
| Eden & Wilde Limited | Wholesale & retail | 2025 |
| Eden Barn Designs & Co Limited | Professional & scientific | 2018 |
| Eden Manor Care Ltd | Health & social care | 2021 |
| Edin Health Ltd | Health & social care | 2025 |
| Edmans Photography Ltd | Professional & scientific | 2022 |
| Edmans Software Solutions Ltd | Information & communication | 2025 |
| Edvon Limited | Information & communication | 2026 |
| Edward Juba Limited | Health & social care | 2023 |
| Edwards Emporium Ltd | Wholesale & retail | 2024 |
| Eeshaanya Limited | Real estate | 2020 |
| Eeyo Limited | Administrative & support | 2016 |
| EFE Mardin Limited | Hospitality | 2020 |
| Ehcp Pro Ltd | Education | 2024 |
| Eighteen Hockey Ltd | Wholesale & retail | 2018 |
| Ejarz Ltd | Wholesale & retail | 2007 |
| Ejjv Apartments Ltd | Real estate | 2020 |
| EKM Property Limited | Real estate | 2024 |
| Elara Pilates Limited | Arts & recreation | 2025 |
| Element Global Property Ltd | Real estate | 2019 |
| Elevate Digital Agency Limited | Professional & scientific | 2025 |
| Elite Care Direct Limited | Wholesale & retail | 2026 |
| Elite Care Recruitments PVT Ltd | Real estate | 2014 |
| Elite Domiciliary Care PVT Ltd | Health & social care | 2018 |
| Elite Upholstery & Design Limited | Manufacturing | 2010 |
| Elizabeth Alexandra Interiors Ltd | Professional & scientific | 2025 |
| Elizabeth John Seymour Limited | Professional & scientific | 2025 |
| Elizabeth Maddox Psychological Therapy Ltd | Health & social care | 2025 |
| Ellco (Sheet Metal) Limited | Real estate | 1980 |
| Elliott & Co Holdings Ltd | Real estate | 2024 |
| Ellis Hillman Partnership Limited | Professional & scientific | 2019 |
| Elmwood Lettings Limited | Real estate | 2018 |
| Elmwood Ventures Limited | Professional & scientific | 2004 |
| ELN Studios Ltd | Manufacturing | 2026 |
| Elneo Ltd | Professional & scientific | 2026 |
| Eluxo Solution Limited | Information & communication | 2025 |
| Elzohi Healthcare Limited | Health & social care | 2020 |
| EM Resolutions Ltd | Manufacturing | 2014 |
| Emaaa Ltd | Professional & scientific | 2023 |
| Embassy Hair Limited | Other services | 2013 |
| Emerging Dreams Ltd | Professional & scientific | 2019 |
| Emkt Ltd | Construction | 2025 |
| Emma Cook Communications Limited | Professional & scientific | 2020 |
| Emmie Lou's Floristry Design Ltd | Wholesale & retail | 2025 |
| Emoba Electric Newcastle Ltd | Wholesale & retail | 2022 |
| Emotions Clinic, Education and Training Centre CIC | Education | 2019 |
| Empire Mobile Solutions Ltd | Information & communication | 2020 |
| Emporium Flooring Holdings Ltd | Financial services | 2025 |
| Empress Design & Print Limited | Manufacturing | 1996 |
| Emre's Account's Ltd | Other services | 2025 |
| Emre's Fast Food Ltd | Hospitality | 2025 |
| Emsa Global Ltd | Wholesale & retail | 2020 |
| EMZ Property Investment Limited | Real estate | 2025 |
| Enable Business Ltd | Information & communication | 2000 |
| Enchanted Toys Limited | Wholesale & retail | 2024 |
| END of Days Gaming Ltd | Wholesale & retail | 2025 |
| Endoexpert Limited | Real estate | 2017 |
| Energicom Ltd | Professional & scientific | 2014 |
| Energy and Building Services Ltd | Construction | 2004 |
| Engineering Inspection Services Ltd | Professional & scientific | 2024 |
| Engineering IT Solutions Limited | Manufacturing | 2020 |
| Enigma HMO Properties Ltd | Real estate | 2026 |
| Enviro-Consults-Uk Ltd | Professional & scientific | 2020 |
| Environmental Essentials Limited | Professional & scientific | 2004 |
| Envirowall Ltd | Construction | 2015 |
| Envisionit Solutions Ltd | Information & communication | 2025 |
| Eookey Limited | Real estate | 2019 |
| EOS Assure Limited | Information & communication | 2025 |
| EOS Risk Group Limited | Administrative & support | 2014 |
| EOS Risk Management Consulting Limited | Professional & scientific | 2015 |
| EOS Risk West Africa Limited | Administrative & support | 2017 |
| Epoch Health & Performance Ltd | Information & communication | 2026 |
| Equidraws Ltd | Arts & recreation | 2025 |
| Equilyze Technologies Limited | Manufacturing | 2025 |
| Equine Products U.K. Limited | Manufacturing | 1981 |
| ERL Properties Ltd | Real estate | 2025 |
| ESA Nuna Ltd | Wholesale & retail | 2026 |
| ESM Cambridgeshire Limited | Arts & recreation | 2024 |
| ESM Norfolk Limited | Arts & recreation | 2024 |
| ESM Sussex Limited | Arts & recreation | 2024 |
| ESM Transport Ltd | Transport & logistics | 2015 |
| ESN Care Ltd | Real estate | 2020 |
| Esobe Medics Limited | Health & social care | 2026 |
| ESP Main Street Limited | Households | 2025 |
| ESP Main Street Management Company Limited | Hospitality | 2025 |
| Essendial Ltd | Professional & scientific | 2025 |
| Essential Cosmetics Ltd | Extraterritorial | 2013 |
| Esuko Services Ltd | Information & communication | 2024 |
| ETA Installations Ltd | Construction | 2019 |
| Ethereal Property Ltd | Extraterritorial | 2024 |
| Etiqus Ltd | Extraterritorial | 2014 |
| Etruria Industrial Museum | Arts & recreation | 2011 |
| Etruscan Estates Limited | Real estate | 2025 |
| EU Tech Solutions Limited | Wholesale & retail | 2023 |
| Euro Process Machinery Ltd | Manufacturing | 2015 |