| Simon Taylor Property Ltd | Construction | 2022 |
| Simon Woodhouse Limited | Real estate | 2024 |
| Simply Construction Cheshire Ltd | Construction | 2020 |
| Simply Helping Ltd | Administrative & support | 2025 |
| Simpson Consulting Solutions Limited | Administrative & support | 2017 |
| Sinnott Group Holdings Limited | Financial services | 2026 |
| Sirigiri Medical Services Limited | Health & social care | 2015 |
| Site Canteen Limited | Hospitality | 2026 |
| SJB Truck Services Limited | Wholesale & retail | 2011 |
| SJO Clinical Limited | Health & social care | 2021 |
| SJS AH2 Venture House Limited | Construction | 2026 |
| SK Sons Limited | Wholesale & retail | 2025 |
| Skerrett Chiropractic and Health Ltd | Health & social care | 2026 |
| Sky High Scaffolding Solutions Limited | Construction | 2022 |
| SL Civil Consultants Limited | Construction | 2015 |
| Slge Holdings Limited | Professional & scientific | 2023 |
| SLI Consulting Limited | Professional & scientific | 2026 |
| SLW Coaching and Development Ltd | Administrative & support | 2025 |
| Smart Academy Uniform Ltd. | Extraterritorial | 2016 |
| SMB Management Services Limited | Professional & scientific | 2008 |
| SMD Logistics Solutions Ltd | Extraterritorial | 2021 |
| Sodeca UK Limited | Extraterritorial | 2016 |
| Soft Dent Smile Limited | Health & social care | 2024 |
| Solutionrooms Limited | Information & communication | 2009 |
| Somerford Oaks Ltd | Real estate | 2022 |
| Soul 360 Limited | Information & communication | 2016 |
| Southlands Nurseries Holdings Ltd | Financial services | 2016 |
| Southlands Nurseries Ltd. | Education | 2003 |
| Sovereign Technical Services Ltd | Information & communication | 2021 |
| Spencroft Saws Limited | Other services | 1988 |
| Sports Timing Services Limited | Extraterritorial | 2004 |
| Sports Timing Systems Limited | Administrative & support | 2009 |
| Squire Bowker & SON Limited | Real estate | 1908 |
| SRE Motors Limited | Wholesale & retail | 2022 |
| Srin Limited | Health & social care | 2006 |
| SRS Demolitions and Groundworking Contractors Limited | Construction | 2013 |
| Staffordshire Access Scaffolding Ltd | Construction | 2009 |
| Staffordshire Alloy Wheel Refurbishments Ltd | Wholesale & retail | 2019 |
| Staffordshire Gynaecology Limited | Health & social care | 2021 |
| Staffordshire Leisure Group Ltd | Hospitality | 2016 |
| Staffordshire Neurology Ltd | Health & social care | 2020 |
| Staffordshire Plumbing and Heating Limited | Construction | 2004 |
| Staffordshire Precision Engineering Holdings Limited | Financial services | 2015 |
| Staffordshire Surgical Ltd | Health & social care | 2020 |
| Staffordshire Vets Limited | Professional & scientific | 2024 |
| Staffs Laser Limited | Professional & scientific | 2017 |
| Starbots Creative Limited | Administrative & support | 2013 |
| Steele’S Pipeline Utilities & Drainage Limited | Construction | 2019 |
| Stellified Ltd | Information & communication | 2019 |
| Steve Emery Holdings Limited | Professional & scientific | 2016 |
| Steve's Smart Repairs Limited | Wholesale & retail | 2013 |
| Stoke Cars Ltd | Wholesale & retail | 2022 |
| Stone Boat Building Chandlery Limited | Administrative & support | 1983 |
| Stonesmith Properties Ltd | Real estate | 2024 |
| Storm Alloys Ltd | Wholesale & retail | 2013 |
| Stove Industry Alliance Limited | Other services | 2008 |
| Strain Auto Solutions Ltd | Wholesale & retail | 2018 |
| Strategic Consulting Services Limited | Professional & scientific | 2018 |
| Stratnav Ltd | Professional & scientific | 2011 |
| Strength Asylum Limited | Arts & recreation | 2013 |
| Strength Asylum Trading Limited | Arts & recreation | 2017 |
| Strimplates UK Limited | Professional & scientific | 2016 |
| Studio 72 Ltd | Arts & recreation | 2026 |
| Suede Vybe House Ltd | Arts & recreation | 2026 |
| Sumitomo Electric Wiring Systems (Europe) Limited | Manufacturing | 1989 |
| Supreme Couriers (Newcastle-U-Lyme) Ltd | Transport & logistics | 1985 |
| SV Security Systems Limited | Administrative & support | 2000 |
| T Clayton Landscaping & Driveways Limited | Administrative & support | 2025 |
| Talukdar Accounting Ltd | Professional & scientific | 2025 |
| Tax Specialists Limited | Professional & scientific | 2018 |
| Taylors Holdings (Stoke-On-Trent) Ltd | Financial services | 2019 |
| Taylors Properties (Stoke-On-Trent) Limited | Real estate | 1960 |
| Tayrona UK Limited | Administrative & support | 2017 |
| TDS Properties Ltd | Real estate | 2020 |
| Tech 3PL Ltd | Transport & logistics | 2004 |
| Teem Jones Holdings Limited | Financial services | 2025 |
| Telanka Ltd | Manufacturing | 2024 |
| Tempest-Bowen 2018 Limited | Financial services | 2018 |
| TEN Oaks Talent Ltd | Administrative & support | 2015 |
| Terasys Ltd | Professional & scientific | 2016 |
| THE Adelphi Vaults Limited | Hospitality | 2016 |
| THE Balterley Beer Company Ltd | Wholesale & retail | 2011 |
| THE Ceiling & Partition Company Limited | Construction | 2007 |
| THE Childrens Playground Company Limited | Construction | 2002 |
| THE Colour Station Ltd | Extraterritorial | 2006 |
| THE CTO Practice Ltd | Professional & scientific | 2010 |
| THE Dog Barber Limited | Administrative & support | 2008 |
| THE Empire Stoke Limited | Hospitality | 2016 |
| THE Extra Smile Limited | Professional & scientific | 2007 |
| THE Indigo Group Ltd. | Professional & scientific | 2008 |
| THE Interior Design Practice Ltd | Construction | 2013 |
| THE Lunar Swing Ltd | Health & social care | 2025 |
| THE Malta Consultancy Limited | Professional & scientific | 2023 |
| THE Nine Shed Limited | Construction | 2019 |
| THE Old Ticket Office Limited | Hospitality | 2025 |
| THE Parking Space Ltd | Other services | 2020 |
| THE Planet of the Vapez (Silverdale) Limited | Other services | 2019 |
| THE Redeemed Christian Church of GOD Newness of Life Christian Centre | Other services | 2019 |
| THE Rehab Collective Limited | Health & social care | 2025 |
| THE Sunlight Group Limited | Construction | 2020 |