| 1ST AID + Limited | Health & social care | 2010 |
| 24-7 Patient Care Ostomy Supplies Limited | Health & social care | 2014 |
| 3C Group (UK) Ltd | Administrative & support | 2007 |
| 46 George Court Management Company Limited | Real estate | 2017 |
| 4SYEDS Ltd | Wholesale & retail | 2021 |
| A Burrows Services Limited | Construction | 2014 |
| A.M Industrial Medical Ltd | Wholesale & retail | 2025 |
| A.P. Mitchell Building Services Limited | Construction | 2005 |
| A.P. Mitchell Developments Ltd | Construction | 2013 |
| Abex Design Ltd | Professional & scientific | 2021 |
| Absolute PPE & Safety Super Store Ltd | Wholesale & retail | 2023 |
| Accountabelle Limited | Education | 2024 |
| Accuro Underwriting Ltd | Financial services | 2000 |
| Accutime Limited | Wholesale & retail | 2007 |
| Acorn Garden Design Ltd | Construction | 2023 |
| Acorns to Oaks Silvan Limited | Construction | 2020 |
| ACS Homes Limited | Real estate | 2007 |
| Action Kids Courses Limited | Education | 2011 |
| Adsideu Holdings Limited | Real estate | 2021 |
| Adventurous Investors Ltd | Real estate | 2022 |
| AEL Heat Exchangers UK Ltd | Wholesale & retail | 2019 |
| AFK Implants Ltd | Health & social care | 2025 |
| Afya Sports Training Ltd | Arts & recreation | 2013 |
| AIM for the Sky Ltd | Arts & recreation | 2012 |
| AJK Accountancy Services Limited | Professional & scientific | 2013 |
| AJK Design and Build Limited | Construction | 2015 |
| AL Shaibi Ltd | Real estate | 2012 |
| Alastair Sanderson Consulting Limited | Professional & scientific | 2025 |
| Alfred H Knight Limited | Professional & scientific | 1988 |
| ALL American Limited | Administrative & support | 2018 |
| ALL American Wirral Ltd | Administrative & support | 2025 |
| ALL Your Cctv Limited | Administrative & support | 2021 |
| ALL Your Comms Limited | Information & communication | 2014 |
| Alma Education Ltd | Education | 2026 |
| Alpha Plant and Equipment UK Ltd | Construction | 2024 |
| Altus Technical Group Ltd | Financial services | 2018 |
| Altus Technical Services Ltd | Construction | 2016 |
| AM Partnership Ltd | Information & communication | 2013 |
| AM Sports Massages Limited | Other services | 2023 |
| AM2 Education Ltd | Education | 2024 |
| Ambient Comfort Solutions Ltd | Construction | 2020 |
| Ambulo Ltd | Wholesale & retail | 2011 |
| Ames Property Holdings Limited | Financial services | 2021 |
| Anchor Advisory Ltd | Professional & scientific | 2025 |
| Antifire Limited | Wholesale & retail | 1993 |
| Antony J Bush Limited | Health & social care | 2007 |
| Apex Auto Group Limited | Wholesale & retail | 2026 |
| Aquila Consultancy Ltd | Professional & scientific | 2023 |
| Aquila Rise CIC | Education | 2024 |
| Arboreal Holdings Ltd | Real estate | 2023 |
| ARP Renovations Ltd | Construction | 2024 |
| Artemis Media and Strategic Counsel Limited | Professional & scientific | 2023 |
| Artemis Media Profile Limited | Professional & scientific | 2011 |
| Ashtree Farm Court Limited | Households | 1999 |
| Asjj Services Ltd | Administrative & support | 2019 |
| Asnf Industrial Limited | Construction | 2023 |
| Aspire Electrical NT Services Ltd | Construction | 2024 |
| Astute Transport Design Limited | Transport & logistics | 2025 |
| Astute Transport Planning Limited | Transport & logistics | 2024 |
| ATC Financial Planning Ltd | Professional & scientific | 2020 |
| Autaris Workforce Compliance Ltd | Information & communication | 2026 |
| Autoshore Ltd | Professional & scientific | 2022 |
| Avante Forwarding Ltd | Transport & logistics | 2022 |
| Aventsys Limited | Information & communication | 2005 |
| Avro Fleet Ltd | Wholesale & retail | 2025 |
| Ayleo Scientific Ltd | Professional & scientific | 2025 |
| Azure Commercial Ltd | Real estate | 2020 |
| Azure Health Ltd | Health & social care | 2019 |
| Back to Nature Organic Foods Ltd | Wholesale & retail | 2025 |
| Backford Brook Farm Ltd | Agriculture & fishing | 2018 |
| Barnston Towers Management Company Limited | Professional & scientific | 2025 |
| Basedata Technologies Limited | Information & communication | 2003 |
| BASS51 Limited | Administrative & support | 2024 |
| Bathroom Bay Ltd | Wholesale & retail | 2020 |
| BCW Lettings Ltd | Real estate | 2021 |
| Beechways Management Limited | Households | 1998 |
| Belvedere Park (Neston) Management Company Limited | Households | 2014 |
| BEN Turley Design Limited | Professional & scientific | 2025 |
| Benco Electrical Services Limited | Manufacturing | 2003 |
| Benjamin Morris & Co Limited | Professional & scientific | 2010 |
| Benzon Ventures Ltd | Professional & scientific | 2023 |
| Beresford Management (Stretford) Limited | Households | 1996 |
| Bettsy & Co Limited | Other services | 2017 |
| Big Day Event Tipis and Marquees Limited | Administrative & support | 2021 |
| Big JOB Ltd | Real estate | 2017 |
| Bindyshahconsulting Limited | Professional & scientific | 2009 |
| Bliss Bridal Ltd | Wholesale & retail | 2021 |
| BLR Diagnostics Ltd | Wholesale & retail | 2020 |
| Bluebeach Properties Limited | Real estate | 1995 |
| Bluebell Tearooms/ Cafe Ltd | Hospitality | 2023 |
| Bold International Properties Limited | Real estate | 1990 |
| BOX125 Ltd | Wholesale & retail | 2024 |
| Brad Donelan Sporting Enterprises Limited | Education | 2009 |
| Braeside Construction Limited | Construction | 2019 |
| Brewers Arms Neston Ltd | Hospitality | 2023 |
| Brift Properties Ltd | Real estate | 2025 |
| Bright Evergreens Ltd | Wholesale & retail | 2020 |
| Brittany Estates Limited | Real estate | 2018 |
| Brookcomm Limited | Information & communication | 2020 |
| Brown Bear Coffee Company Limited | Hospitality | 2023 |