| Quantum Packaging Limited | Manufacturing | 2010 |
| Quenchable Limited | Education | 2018 |
| Qwik Lube Limited | Wholesale & retail | 2010 |
| R&A Musselburgh Ltd | Wholesale & retail | 2026 |
| R&R Campbell Holdings Limited | Health & social care | 2016 |
| R.A.C.E. Core Ltd | Construction | 2026 |
| R.M. Taxis Ltd. | Transport & logistics | 1999 |
| Rachel Rickman Counselling and Psychotherapy Ltd | Health & social care | 2022 |
| Radial Turbines Ltd | Professional & scientific | 2020 |
| Ramsey Electrical Services Limited | Construction | 2022 |
| Ravelston House Ltd | Hospitality | 2017 |
| Raven Resourcing Ltd | Professional & scientific | 2012 |
| RAY Mays Ltd | Professional & scientific | 2019 |
| RAZ Express Ltd | Wholesale & retail | 2021 |
| Rb.It Specialists Limited | Information & communication | 2022 |
| RC Carlyle Properties Ltd | Real estate | 2025 |
| RE Marcomms Limited | Professional & scientific | 2014 |
| RE Nails Ltd | Health & social care | 2020 |
| Re-Play IT Ltd | Wholesale & retail | 2017 |
| Re-Play IT Scotland Ltd | Wholesale & retail | 2020 |
| Rebber Limited | Other services | 2023 |
| Reclaimed and Vintage Limited | Wholesale & retail | 2009 |
| Red Mcgregor Marketing Limited | Professional & scientific | 2004 |
| Redpath Plumbing Ltd | Construction | 2007 |
| Redweb Holdings Limited | Transport & logistics | 2017 |
| Regal Radiance Ltd | Administrative & support | 2024 |
| Renaissance Care (NO 3) Limited | Health & social care | 2011 |
| Renaissance Care (NO 4) Limited | Health & social care | 2011 |
| Renaissance Care (NO 5) Limited | Health & social care | 2013 |
| Renaissance Care (NO 6) Ltd. | Health & social care | 2016 |
| Renaissance Care (NO 7) Limited | Health & social care | 1997 |
| Renaissance Care (NO 8) Limited | Health & social care | 2004 |
| Renaissance Care (NO 9) Limited | Health & social care | 2017 |
| Renaissance Care (NO. 10) Ltd | Health & social care | 2019 |
| Renaissance Care (NO1) Limited | Health & social care | 2011 |
| Renaissance Care (NO2) Limited | Health & social care | 2011 |
| Renaissance Care (Scotland) Limited | Health & social care | 1998 |
| Renaissance Care (UK) Ltd | Health & social care | 2010 |
| Rendezvous Kelso Ltd | Hospitality | 2020 |
| RG Barber Ltd | Other services | 2025 |
| RGS Braidwood Limited | Professional & scientific | 2024 |
| Rhyz Ltd | Other services | 2020 |
| Ride & Grind Ltd | Manufacturing | 2024 |
| Right Hand Person Ltd | Professional & scientific | 2024 |
| Ritchie Denholm Ltd | Construction | 2026 |
| Riverside Medical Practice LLP | | 2017 |
| Riverside MP Limited | Real estate | 2012 |
| RJ Building Surveying Ltd | Professional & scientific | 2018 |
| RM Drilling Ltd | Mining & quarrying | 2018 |
| RMC Innovation Consulting Ltd | Professional & scientific | 2025 |
| Robbie Vault Ltd | Wholesale & retail | 2026 |
| Robert Kilgour Ltd | Professional & scientific | 2015 |
| Robert P. Slight & Sons (Holdings) Limited | Financial services | 2022 |
| Robert P. Slight & Sons Limited | Manufacturing | 1958 |
| Roofing Plus + Ltd | Construction | 2026 |
| Rose and Revelry Ltd | Extraterritorial | 2024 |
| Roseisle Estates Limited | Wholesale & retail | 1984 |
| Roslin Building & Paving Services Ltd | Construction | 2025 |
| Ross Plumbing and Heating Ltd | Construction | 2024 |
| Roxie and Ollie Ltd | Wholesale & retail | 2025 |
| RP Slight & Sons EOT Trustee Limited | Financial services | 2024 |
| RS Barbers Musselburgh Ltd | Other services | 2025 |
| Ruby Private Hire Ltd | Extraterritorial | 2015 |
| Russell Hansen Limited | Real estate | 2019 |
| Rustic Roots Limited | Administrative & support | 2017 |
| Ryzn Ltd | Other services | 2024 |
| S & K Property Holdings Ltd | Administrative & support | 2017 |
| S M Mitchell Limited | Administrative & support | 2010 |
| S&N Taxis Limited | Transport & logistics | 2000 |
| S5 Enterprises Ltd | Real estate | 2026 |
| Sahara (Scotland) Ltd | Wholesale & retail | 2023 |
| Sale Sense Limited | Wholesale & retail | 2025 |
| Saleemco Limited | Hospitality | 2025 |
| Saltire Network Services Limited | Construction | 2019 |
| Samaz Consultancy Ltd | Professional & scientific | 2025 |
| Sandroaly Ltd | Hospitality | 2022 |
| SB Supports the Union Ltd | Other services | 2017 |
| Scallyco Ltd | Hospitality | 2024 |
| Schi Ltd | Construction | 2018 |
| Scot Tile Contracts Limited | Construction | 1994 |
| Scotglen Limited | Transport & logistics | 2001 |
| Scotia Property Maintenance Limited | Construction | 2016 |
| Scotia Property Projects Limited | Construction | 2022 |
| Scots International Ltd | Wholesale & retail | 2020 |
| Scotspray Bodyshop Ltd | Wholesale & retail | 2015 |
| Scott Test Solutions Ltd | Information & communication | 2025 |
| Scott-Eco Builders Ltd | Construction | 2016 |
| Scottish Gardens Network Ltd | Arts & recreation | 2014 |
| Scottish Mountain Gear Limited | Manufacturing | 2003 |
| Sdsl Holdings Ltd | Financial services | 2026 |
| Sdsl Investments Ltd | Real estate | 2026 |
| Selling Knowhow Limited | Real estate | 2016 |
| Sentient-Things Limited | Professional & scientific | 2014 |
| Sharkey (Group) Limited | Administrative & support | 2014 |
| Sharples Engineering Services Ltd | Professional & scientific | 2019 |
| Sharples Swim Services Limited | Education | 2016 |
| Sheetloom Limited | Information & communication | 2024 |
| Shish Grill Musselburgh Ltd | Hospitality | 2026 |
| Shish Mahal Musselburgh Limited | Hospitality | 2024 |
| Si.Blair Ltd | Real estate | 2025 |