| DKL Design Limited | Professional & scientific | 2015 |
| Dog Days Films Ltd | Information & communication | 2012 |
| Domestic Tank Services Limited | Construction | 2016 |
| Donlane Limited | Water & waste | 1997 |
| Dorn Hill Machine Services Ltd | Manufacturing | 2015 |
| DP Flow Ltd | Manufacturing | 2013 |
| DPN Services Ltd | Administrative & support | 2016 |
| DR Emily Cary Medical Limited | Administrative & support | 2023 |
| DR Hannah Healy Medical Limited | Administrative & support | 2023 |
| DR Susanne Ahlund Medical Limited | Administrative & support | 2023 |
| DR Treasury Ltd | Professional & scientific | 2015 |
| Dragon Commodities Ltd | Wholesale & retail | 2020 |
| Dudfield Farm Buildings Ltd | Construction | 2011 |
| DXF Design Limited | Professional & scientific | 2012 |
| Dynamic Performance Hub Ltd | Arts & recreation | 2018 |
| E7 Limited | Administrative & support | 2020 |
| Earnshaw Escapes Ltd | Real estate | 2026 |
| Easy Charge EV Limited | Construction | 2019 |
| Eaton International Limited | Professional & scientific | 2023 |
| Eco VAC Limited | Construction | 2012 |
| ED Dove Sports Media Limited | Information & communication | 2016 |
| Eden Hall Day Spa Limited | Health & social care | 2001 |
| EDS Property Ltd | Real estate | 2016 |
| EJR Cotswold Ltd | Real estate | 2018 |
| Elizabeth FRY Road Limited | Construction | 2004 |
| Emmela Limited | Wholesale & retail | 2020 |
| Enchanted Software Limited | Information & communication | 2017 |
| Engage and Improve Ltd | Professional & scientific | 2023 |
| Engineering Partners (Poole) LLP | | 2008 |
| Entrigue Ltd | Professional & scientific | 2024 |
| Epsp Ltd | Professional & scientific | 2019 |
| EQ Bands Ltd | Arts & recreation | 2022 |
| Equine Medicine on the Move Limited | Professional & scientific | 2004 |
| Everything Organised Ltd | Other services | 2017 |
| Farmlane Limited | Real estate | 1999 |
| Farmstead Moreton Ltd | Financial services | 2024 |
| FG4 UK Limited | Wholesale & retail | 2011 |
| Finiken Ltd | Hospitality | 2020 |
| Fintellect Limited | Professional & scientific | 1991 |
| Fintellect Publishing Limited | Information & communication | 2000 |
| First Things Ltd | Professional & scientific | 2020 |
| Firstlight Digital Ltd | Information & communication | 2026 |
| Fishers Lock Limited | Real estate | 2012 |
| Florent Enterprises Ltd | Real estate | 1944 |
| Fluid Process Instruments Limited | Extraterritorial | 2017 |
| Food & Beverage Solutions (UK) Ltd | Manufacturing | 2022 |
| Forbes Investments Limited | Financial services | 2024 |
| Forge Conducting Limited | Education | 2025 |
| Fosseway Estate Residents Association Limited | Households | 1972 |
| Fosseway Fields Ltd | Arts & recreation | 2023 |
| Fosseway Investments Limited | Real estate | 2022 |
| Fosseway Performance Ltd | Manufacturing | 2013 |
| Four Shires Fencing Ltd | Construction | 2026 |
| Frovi Limited | Manufacturing | 2015 |
| Fully Moto Ltd | Wholesale & retail | 2024 |
| Futureview Consulting Ltd | Professional & scientific | 2021 |
| G W Interiors Holdings Limited | Real estate | 2008 |
| Gembaworks Consultancy Ltd | Professional & scientific | 2026 |
| Gemma Pimm Photography Ltd | Professional & scientific | 2024 |
| Genesis Bidco Limited | Financial services | 2025 |
| Genesis Devco 1 Limited | Financial services | 2025 |
| Genesis Devco 2 Limited | Financial services | 2025 |
| Genesis Devco 3 Limited | Financial services | 2025 |
| Genesis Devco 4 Limited | Financial services | 2025 |
| Genesis Midco 1 Limited | Financial services | 2025 |
| Genesis Midco 2 Limited | Financial services | 2025 |
| George W. Davies Ltd | Wholesale & retail | 2015 |
| Geowisdom Ltd | Mining & quarrying | 2025 |
| GGB Investments Ltd | Real estate | 2017 |
| GHF Advisory Services Ltd | Professional & scientific | 2020 |
| GHF Property Holdings Ltd | Real estate | 2021 |
| GI Electrical Solutions Ltd | Construction | 2023 |
| Gig-Arts | Arts & recreation | 2013 |
| Glen Wind Farm Company Limited | Professional & scientific | 2014 |
| Go Barefoot Limited | Manufacturing | 2011 |
| Godson Construction Limited | Construction | 2022 |
| Godson Dillon Construction Limited | Construction | 2022 |
| Gold ARC Solutions Ltd | Wholesale & retail | 2025 |
| Gold Star Tours Ltd | Transport & logistics | 2020 |
| Goose and Varley Limited | Wholesale & retail | 2026 |
| GP1X Ltd | Administrative & support | 2019 |
| Grand CRU Creative Limited | Manufacturing | 2023 |
| Grasett Services Limited | Professional & scientific | 2018 |
| Grasshopper Investments (Holding) Ltd | Financial services | 2019 |
| Grasshopper Investments Ltd | Financial services | 2019 |
| Grasshopper Trading (Holding) Ltd | Financial services | 2019 |
| Grasshopper Trading Ltd | Financial services | 2019 |
| Gravity Automotive Ltd | Wholesale & retail | 2025 |
| Great British Rare Breed PIG Company Ltd | Wholesale & retail | 2018 |
| Great Western Blockley Ltd | Hospitality | 2018 |
| Green Gills Ltd | Professional & scientific | 2021 |
| Green Impact Investors Limited | Financial services | 2018 |
| Green&Blue Nature Ltd | Manufacturing | 2023 |
| Greengrass Gardening Ltd | Administrative & support | 2012 |
| Griffin Carpentry & Construction Ltd | Construction | 2020 |
| Grosvenor House Accounting Services Limited | Administrative & support | 2007 |
| Grove House Developments (Cotswolds) Ltd | Construction | 2019 |
| Growth Property Limited | Real estate | 2012 |
| GSK Connect Ltd | Information & communication | 2005 |
| Guiding Light Architecture Ltd | Professional & scientific | 2020 |