| Bruce Sutherland & Co. | Professional & scientific | 1985 |
| Bruce Sutherland Inc. | Financial services | 1978 |
| Buckingham Research and Consultancy Limited | Financial services | 2025 |
| Built Form Resource Ltd | Professional & scientific | 2011 |
| Business Village Limited | Real estate | 2002 |
| BY Day DOT Limited | Wholesale & retail | 2024 |
| C.A.B&Partners Limited | Hospitality | 2020 |
| C.A.Stanley Ltd | Hospitality | 2018 |
| Calarel Joinery Limited | Construction | 2016 |
| Campbell Enterprises Ltd | Financial services | 2025 |
| Campbell Gibbons Consulting Ltd | Professional & scientific | 2021 |
| Campden Consultancy Ltd | Professional & scientific | 2016 |
| Cassiopeia Holdings Ltd | Professional & scientific | 2015 |
| Castaway Kitchen Limited | Hospitality | 2017 |
| Cathedral Property Co. Limited(The) | Construction | 1979 |
| CD Groundworks & Landscaping Ltd | Administrative & support | 2018 |
| Centinix Limited | Information & communication | 2009 |
| CGB Developments Limited | Financial services | 2012 |
| Chapel Street Garages Ltd | Construction | 2013 |
| Chill Technology Ltd | Professional & scientific | 2022 |
| Chris Leach DRY Stone Walling & Landscaping Ltd | Construction | 2013 |
| Christopher Tombs Limited | Wholesale & retail | 2009 |
| Churchtown Cottages Residents Association Limited | Households | 1997 |
| Ckaiser and Associates Limited | Information & communication | 2015 |
| Claire Bailey Gynaecology Limited | Health & social care | 2017 |
| Classic Bindings Limited | Arts & recreation | 1996 |
| Cleaning Industries Partnership LLP | | 2017 |
| Clifton Cinema (Fallings Park) Limited | Real estate | 1936 |
| Clive Wadey Paxford Garage Limited | Wholesale & retail | 2009 |
| Coating Technologies EOT Trustee Limited | Professional & scientific | 2025 |
| Coating Technologies Limited | Manufacturing | 1979 |
| Coffee Establishment Limited | Professional & scientific | 2012 |
| Colin Baker Installations Limited | Construction | 2019 |
| Colwell Holdings Limited | Financial services | 2025 |
| Commercial Consultants - Europe Limited | Education | 1986 |
| Conalliance UK Limited | Professional & scientific | 2015 |
| Confidence IS Glue Limited | Health & social care | 2008 |
| Contented Dementia Trust | Administrative & support | 2001 |
| Corporate Business Ltd | Administrative & support | 2004 |
| Cotswold Bodyshop Supplies Limited | Administrative & support | 2016 |
| Cotswold Calendar Company Limited | Information & communication | 2010 |
| Cotswold Falconry Limited | Arts & recreation | 2008 |
| Cotswold Farm Escapes Ltd | Hospitality | 2025 |
| Cotswold Friends | Health & social care | 2004 |
| Cotswold Garden Tours Ltd | Administrative & support | 2020 |
| Cotswold Green Energy Limited | Construction | 2011 |
| Cotswold Laser Cleaning Ltd | Administrative & support | 2025 |
| Cotswold Markets Limited | Wholesale & retail | 2013 |
| Cotswold NUT Company Limited | Agriculture & fishing | 2023 |
| Cotswold Organic Dairy Limited | Manufacturing | 2022 |
| Cotswold Partnership Ltd | Wholesale & retail | 2025 |
| Cotswold Peer Support Limited | Other services | 2025 |
| Cotswold Petrol Limited | Wholesale & retail | 2017 |
| Cotswold Powerflush Ltd | Construction | 2022 |
| Cotswold Property Services & Management Ltd | Real estate | 2021 |
| Cotswold Property Solutions Ltd | Construction | 2024 |
| Cotswold Renovations by Stephen Arkell Limited | Construction | 2019 |
| Cotswold Shutter Co Ltd | Manufacturing | 2020 |
| Cotswold Vehicle Solutions Ltd | Wholesale & retail | 2019 |
| Cotswolds & Exceed Properties Ltd | Real estate | 2020 |
| Cotswolds Aerial Photography Ltd | Information & communication | 2020 |
| Cotswolds Consortium Ltd | Administrative & support | 2024 |
| Cotswolds Guided Tours Ltd | Administrative & support | 2018 |
| Cotswolds Hideaways Limited | Hospitality | 2007 |
| Cotswolds Kitchens Limited | Construction | 2024 |
| Country Curtains Limited | Manufacturing | 2016 |
| Coxwell Chase Limited | Real estate | 2025 |
| Crisis Services Ltd | Information & communication | 2026 |
| Cross Street. CH. Properties Limited | Hospitality | 2000 |
| Crossgrain Ltd | Manufacturing | 2025 |
| Curfew Hair Ltd | Other services | 2021 |
| Cvas Garage Limited | Wholesale & retail | 2024 |
| CY Safety Limited | Professional & scientific | 2017 |
| Cyber Hutt Ltd | Information & communication | 2025 |
| Cyberdawn Limited | Information & communication | 2022 |
| Cyberstart Limited | Information & communication | 2022 |
| Cynara UK 2 Limited | Administrative & support | 2020 |
| D & M Forestry Services Ltd | Agriculture & fishing | 2017 |
| D J G T Services Limited | Professional & scientific | 2024 |
| D T Newbould Road Contractor and Groundworks Ltd | Construction | 2011 |
| Dandelion Investments Limited | Real estate | 2012 |
| Daniel Blyth Limited | Health & social care | 2008 |
| David Fenton Consultancy Ltd | Professional & scientific | 2019 |
| Daylesford Organic Limited | Wholesale & retail | 1999 |
| Daylesford Stays LLP | | 2020 |
| DBI Certification-Uk Limited | Professional & scientific | 2020 |
| DC Decorations and Renovations Ltd | Construction | 2021 |
| DDT Transport Ltd | Transport & logistics | 2026 |
| Deco Interiors Ltd | Wholesale & retail | 2007 |
| Deeley Farms Limited | Agriculture & fishing | 1996 |
| Dekomte DE Temple Kompensator-Technik (UK) Ltd. | Manufacturing | 1996 |
| DEL Maestro Projects Limited | Construction | 2016 |
| Destination India Limited | Administrative & support | 2025 |
| Detail Films Ltd | Arts & recreation | 2013 |
| Development Technical Solutions Limited | Information & communication | 2023 |
| Different Kind Limited | Health & social care | 2021 |
| Digbeth Property Services Ltd | Real estate | 2007 |
| Direct Dash Deliveries Ltd | Transport & logistics | 2026 |
| Ditchford Hill Management Company Limited | Households | 1993 |
| Divergent Minds Directory Ltd | Information & communication | 2026 |