| DJC Software Design Limited | Information & communication | 2020 |
| Djpealing Limited | Professional & scientific | 2016 |
| DM Jones Properties Limited | Real estate | 2020 |
| DMS Security Limited | Administrative & support | 2022 |
| Dolhendre Hydro Limited | Energy | 2012 |
| Downing Apartment Management Company Limited | Professional & scientific | 2015 |
| DPK Commercials Ltd | Wholesale & retail | 2018 |
| DPK Self Storage Ltd | Real estate | 2024 |
| Dragon Locks Ltd | Other services | 2025 |
| Dragon Motor Services Ltd | Wholesale & retail | 2016 |
| Dragon Teamwear Ltd | Wholesale & retail | 2020 |
| Drains Today Ltd | Water & waste | 2009 |
| Drive Way Development Limited | Manufacturing | 2025 |
| Drivecon Ltd | Transport & logistics | 2023 |
| Driven Marketing Services Ltd | Information & communication | 2015 |
| DUB Alchemy Limited | Manufacturing | 2026 |
| Dutton and Peters Limited | Construction | 1958 |
| E-Motive Classic Cars Ltd | Wholesale & retail | 2020 |
| E5 Group Limited | Financial services | 2021 |
| Earth & Lime Limited | Construction | 2003 |
| Eastern Mold Limited | Hospitality | 2016 |
| EB Taverns Limited | Hospitality | 2025 |
| ECC Systems Ltd. | Information & communication | 2011 |
| Eco Heat NW Ltd | Construction | 2025 |
| Eco Heating & Insulation Ltd | Professional & scientific | 2020 |
| Eco Recycling Projects Ltd | Manufacturing | 2025 |
| Ecoprocess Engineering Limited | Manufacturing | 2015 |
| Ecoture Ltd | Education | 2020 |
| Eddys Haircut Ltd | Other services | 2024 |
| Edenshine Delights Limited | Hospitality | 2003 |
| Edge Naturescape CIC | Professional & scientific | 2025 |
| Edzacly Distributions Ltd | Wholesale & retail | 2026 |
| Egmp Limited | Real estate | 2013 |
| Elan Mortgage Solutions Ltd. | Financial services | 2019 |
| Elate Construction Limited | Construction | 2008 |
| Elin Wynt CYF | Energy | 2012 |
| Elite Equestrian Coaching & Events Ltd | Arts & recreation | 2015 |
| Elite PC Systems Limited | Other services | 2004 |
| Elitehelp Limited | Information & communication | 2000 |
| Elizabeth Robertson Services Limited | Health & social care | 2022 |
| Elliott Consulting Ltd | Professional & scientific | 2023 |
| Elliott James Properties Ltd | Real estate | 2020 |
| Ellis's Property Holdings Ltd | Real estate | 2025 |
| Ellwood Contractors Limited | Agriculture & fishing | 2014 |
| ELM Avionics Engineering Limited | Manufacturing | 1997 |
| ELM Kebab Mold Ltd | Hospitality | 2023 |
| ELM Works Group Limited | Professional & scientific | 2023 |
| Emergency Response Limited | Professional & scientific | 2005 |
| EMPORIUMPRINTS28 Ltd | Manufacturing | 2024 |
| Endur Performance Ltd | Arts & recreation | 2023 |
| Endura PEN Limited | Hospitality | 2025 |
| Engineeco Limited | Education | 2025 |
| Enlightenment Group Holdings Ltd | Financial services | 2024 |
| Enlightenment Productions Limited | Information & communication | 2001 |
| Enlightenment Properties Ltd | Real estate | 2010 |
| Equiscope Ltd | Professional & scientific | 2021 |
| Erosa Studios Ltd | Manufacturing | 2025 |
| ERW Goed Homes Ltd | Construction | 2020 |
| Erw-Olchfa Smallholding Ltd | Financial services | 2026 |
| ESA Accountancy Ltd | Professional & scientific | 2018 |
| Essentials Direct Limited | Wholesale & retail | 2010 |
| EUD Limited | Information & communication | 2012 |
| Euro Enterprise Parks Ltd | Professional & scientific | 2007 |
| Euroliters Limited | Manufacturing | 1991 |
| Euroroute Truck Stops Limited | Professional & scientific | 2008 |
| Evarene Limited | Extraterritorial | 2011 |
| Even Keel Counselling Ltd | Health & social care | 2020 |
| Evolve Learning Design Ltd | Professional & scientific | 2019 |
| Evolving Careers Limited | Professional & scientific | 2020 |
| EWM Advisory Ltd | Professional & scientific | 2024 |
| EX Learning Ltd | Professional & scientific | 2025 |
| Excell Supply Ltd. | Administrative & support | 2011 |
| Exceptional Days Limited | Administrative & support | 2024 |
| Exile Brewing Company Ltd | Manufacturing | 2014 |
| Expotron Limited | Manufacturing | 1991 |
| Extension Specialists Warrington Ltd | Construction | 2024 |
| Exuvia Ltd | Other services | 2025 |
| F Van Dijk Ltd | Information & communication | 2025 |
| F&B Mold Ltd | Manufacturing | 2025 |
| F&T Sporting Limited | Arts & recreation | 2025 |
| F.G.Whitley and Sons Co.Limited | Construction | 1961 |
| Facial Aesthetics & Cosmetic Enhancements Ltd | Other services | 2020 |
| Fagan Properties Limited | Real estate | 2009 |
| Fairbank Building Services Limited | Construction | 2015 |
| Famau View Farm Ltd | Agriculture & fishing | 2023 |
| Family Tree Financial Planning Limited | Financial services | 2020 |
| Family Tree Wealth Management Ltd | Financial services | 2020 |
| Farahbluck Ltd | Arts & recreation | 2025 |
| Fareham Associates Limited | Administrative & support | 2014 |
| Farm Fit Club (Mold) Limited | Arts & recreation | 2026 |
| Farm Fit Club Limited | Other services | 2024 |
| Faro 1 Ltd | Hospitality | 2025 |
| Farwa Enterprises Ltd | Hospitality | 2026 |
| FAY Elizabeth Counselling Services Ltd | Health & social care | 2026 |
| FEJ Consultancy Ltd | Administrative & support | 2024 |
| Ferndale Rental Properties Limited | Real estate | 2025 |
| Fernmount Properties Ltd | Real estate | 2021 |
| Ffrith Famau CIC | Education | 2022 |
| Ffynnon-Y-Cyff Holdings Limited | Agriculture & fishing | 2024 |
| FG2 Ltd | Other services | 2015 |