| 1019 Limited | Arts & recreation | 2012 |
| 108 Rental Ltd | Real estate | 2019 |
| 1ST for Fencing (Erectors and Consultants) Limited | Construction | 2010 |
| 1ST for Fencing Limited | Extraterritorial | 2017 |
| 4CAPACITIES Ltd. | Education | 2013 |
| A & L Northern Properties Ltd | Real estate | 2026 |
| A S Walls Limited | Professional & scientific | 2007 |
| A. B. Wight Ltd | Manufacturing | 2018 |
| A.W.M.D. Limited | Wholesale & retail | 2004 |
| Absolute Assistants Limited | Health & social care | 2020 |
| Ace Carpet Cleaning Ltd | Administrative & support | 2020 |
| Adam Family Assets LLP | | 2013 |
| ADN Promotions Limited | Information & communication | 2000 |
| Aitchison Roofing & Joinery Ltd | Construction | 2026 |
| AJ Business Services Ltd | Professional & scientific | 2019 |
| ALE Water Valley Limited | Other services | 2015 |
| ALH Aesthetics Limited | Health & social care | 2022 |
| Allied Truck & Trailer Components Limited | Wholesale & retail | 2024 |
| Andy Purves Limited | Professional & scientific | 2003 |
| ARK Renovations Ltd | Construction | 2021 |
| Atelier Twenty SIX Ltd | Other services | 2025 |
| Athena Safety Limited | Professional & scientific | 2008 |
| B.Thom Joinery Ltd | Construction | 2013 |
| B68 Digital Ltd | Information & communication | 2026 |
| Bake and Inflate Ltd | Hospitality | 2024 |
| Balcrie Medical Ltd | Health & social care | 2016 |
| Bcca Accountants Limited | Professional & scientific | 2019 |
| Beaverhall Properties Limited | Real estate | 1983 |
| Becoming Rewired Ltd | Professional & scientific | 2026 |
| Begley Holdings Limited | Hospitality | 2020 |
| Bell Financial Planning Limited | Financial services | 2010 |
| Best Bar None Borders Limited | Hospitality | 2015 |
| Blackstar Advisory Limited | Real estate | 2020 |
| Bludgeon Inc Limited | Arts & recreation | 2010 |
| BLV Property Limited | Real estate | 2017 |
| Border Arborists Ltd | Agriculture & fishing | 2026 |
| Border Mowbots Ltd | Wholesale & retail | 2025 |
| Border Pool and Gaming Ltd | Arts & recreation | 2025 |
| Border Potatoes Limited | Wholesale & retail | 2005 |
| Borders Book Festival | Arts & recreation | 2006 |
| Borders Engineering Services Ltd | Wholesale & retail | 2015 |
| Borders GUN Room Limited | Wholesale & retail | 2013 |
| Borders Hotels Limited | Hospitality | 1990 |
| Borders Occupational Therapy Ltd | Health & social care | 2023 |
| Borders Pet Crematorium Limited | Other services | 2018 |
| Borders Sport and Leisure Enterprises Limited | Arts & recreation | 2003 |
| Borders Third Sector Interface Ltd | Education | 2023 |
| BRA Holdings Ltd | Real estate | 2025 |
| Braeburn Melrose Ltd | Real estate | 2026 |
| Braveheart Industries | Other services | 2014 |
| Bridge Homes LLP | | 2014 |
| Bruce Mallen Limited | Construction | 2019 |
| Bunyan & Bell Joiners Limited | Manufacturing | 2006 |
| Burgh Fencing Limited | Agriculture & fishing | 2012 |
| Butterfly Melrose Ltd | Wholesale & retail | 2020 |
| Caledonia Timber Ltd | Extraterritorial | 2021 |
| Callaghan’S Transport Ltd | Transport & logistics | 2018 |
| Camieston Renewables Limited | Energy | 2016 |
| Canongate Consultants Ltd. | Administrative & support | 2015 |
| Capercaillie Cruisers Limited | Administrative & support | 2003 |
| Carlton Scott (Digital) Limited | Professional & scientific | 2026 |
| Carlton Scott Ltd | Professional & scientific | 2023 |
| Carpe Diem Services Ltd | Administrative & support | 2025 |
| Charlesfield Farms Limited | Agriculture & fishing | 1966 |
| Citidogs Lead & Learn Ltd | Education | 2025 |
| Citidogs Ltd | Other services | 2010 |
| Coffee Constructs Ltd | Wholesale & retail | 2022 |
| Confluence Consulting Limited | Professional & scientific | 2006 |
| Connected Farms Ltd | Information & communication | 2022 |
| Cooks Van Hire Limited | Administrative & support | 2014 |
| Cotfield Farmers | | 1988 |
| Cotfield Farmers | | 1999 |
| Creative Hair Studio Ltd | Other services | 2019 |
| Cross Country Theatre Company Limited | Arts & recreation | 2000 |
| Crossroads (Dunfermline) Care Attendant Scheme | Other services | 1998 |
| Csbell Developments Limited | Construction | 2021 |
| Csfinelineaesthetics Ltd | Health & social care | 2021 |
| CT Executive Ltd | Administrative & support | 2019 |
| D a Maxwell & Company | | 2001 |
| D.E. Fencing Ltd. | Construction | 1993 |
| Darnick Care Ltd | Health & social care | 2024 |
| Darnick Management Ltd | Real estate | 2024 |
| Dean Park Investments Limited | Real estate | 2018 |
| Deans Group Awnings Ltd | Construction | 2023 |
| Defreeze Ltd | Professional & scientific | 2023 |
| Dimpleknowe Golf Club Limited | Construction | 2002 |
| Dingleton Road Development Limited | Other services | 2022 |
| Distrikt Ltd | Wholesale & retail | 2025 |
| DJ Crawford Holdings Ltd | Professional & scientific | 2024 |
| DJM Motor Services Ltd | Wholesale & retail | 2025 |
| DLX Performance Ltd | Education | 2026 |
| DM Engineering Solutions Ltd | Professional & scientific | 2017 |
| Dodds Property & Construction Limited | Construction | 2023 |
| Drem Limited | Professional & scientific | 2005 |
| Dryburgh Abbey Hotel Limited | Hospitality | 2007 |
| Ecosse Communications Ltd. | Information & communication | 2018 |
| Edin Cabs Ltd | Transport & logistics | 2025 |
| Edinburgh Sports Festival Ltd | Arts & recreation | 2024 |
| Eildon Catering Ltd | Hospitality | 2026 |
| Eildon Consulting Ltd | Information & communication | 2010 |