| Eildon E-Bike Co. Ltd | Wholesale & retail | 2025 |
| Eildon Parke Ltd | Real estate | 2025 |
| Eildon Partners Ltd | Professional & scientific | 2025 |
| Emotive Click Ltd. | Professional & scientific | 2024 |
| Epic Eats Event Catering Ltd | Hospitality | 2025 |
| Eribe Knitwear Limited | Manufacturing | 2012 |
| Eribe Manufacturing Ltd | Manufacturing | 2019 |
| Essendine Scotland Limited | Other services | 2025 |
| ETH UN Limited | Information & communication | 2025 |
| Ettrickhaugh Development Company Limited | Other services | 2022 |
| F&F Prestige Limited | Wholesale & retail | 2025 |
| FCL Consultancy Ltd | Education | 2022 |
| Ferniehurst Gynaecology Services Limited | Health & social care | 2016 |
| Firm of a L Common Limited | Agriculture & fishing | 2019 |
| Firth Farm | | 1998 |
| Flavours UN Limited | Manufacturing | 2018 |
| Flux Choice Ltd | Wholesale & retail | 2020 |
| Fonn Creative Limited | Professional & scientific | 2025 |
| Fraser Securities Limited | Wholesale & retail | 2012 |
| FTC Kitchen Limited | Hospitality | 2021 |
| FTH Ltd | Real estate | 2026 |
| G D Limited | Wholesale & retail | 2015 |
| G Forbes Joinery Limited | Construction | 2019 |
| G. Marshall (Tractors) Limited | Wholesale & retail | 2003 |
| G.L.S. Limited | Construction | 1989 |
| Gainline Management Ltd | Information & communication | 2020 |
| Gattonside Mains Limited | Agriculture & fishing | 2001 |
| Geogray Ltd | Other services | 2026 |
| George Marshall (Ancrum) Limited | Agriculture & fishing | 1957 |
| GG26 Limited | Real estate | 2025 |
| Gileth Consulting Limited | Professional & scientific | 2020 |
| Glen DAN Limited | Agriculture & fishing | 2000 |
| Glen Nord Limited | Agriculture & fishing | 2000 |
| Go Wild Scotland Community Interest Company | Arts & recreation | 2020 |
| Good Days Club Ltd | Hospitality | 2024 |
| GV Events Borders Ltd | Hospitality | 2023 |
| H&N Olesen Limited | Agriculture & fishing | 2014 |
| Healthoutfit Limited | Professional & scientific | 2000 |
| Helmtec Consulting Limited | Professional & scientific | 2023 |
| Hoebridge Inn Ltd | Hospitality | 2014 |
| Hoebridge Stables Cottage Limited | Households | 2018 |
| Homes on Tweed Ltd | Real estate | 2022 |
| Hosta Holdings Limited | Financial services | 2018 |
| Hunter Stables Restaurant Limited | Hospitality | 2016 |
| Iain Smith & Partners Limited | Professional & scientific | 1986 |
| Iceflo Limited | Information & communication | 2019 |
| Infiniteaj Limited | Wholesale & retail | 2024 |
| IQX Limited | Information & communication | 1997 |
| Irvine Property Investment Services Limited | Real estate | 2018 |
| Istanbul Takeaway Ltd | Hospitality | 2020 |
| J & C Bell Developments Limited | Construction | 2004 |
| J & J Tait Ltd | Construction | 2012 |
| J Lyon Forestry & Groundworks Ltd | Agriculture & fishing | 2023 |
| J Norman Forestry Ltd | Agriculture & fishing | 2021 |
| J. a. Wilson Contractors Ltd | Construction | 2011 |
| J. Campbell & Sons | | 1989 |
| Jack Hunter Electricians Ltd | Construction | 2018 |
| Jackie Johnston Limited | Agriculture & fishing | 2013 |
| Jackson Distillers Limited | Manufacturing | 2017 |
| Jackson Howard LLP | | 2009 |
| Jaggy Nettle Ltd | Manufacturing | 2008 |
| James Agent (East) Ltd | Real estate | 2025 |
| James Agent Ltd | Real estate | 2020 |
| James Denne Limited | Real estate | 2024 |
| Jasleot Ltd | Professional & scientific | 2022 |
| JNB Property Limited | Real estate | 2022 |
| John Swan & Sons Limited | Financial services | 1911 |
| John Swan Limited | Wholesale & retail | 2003 |
| Johnston Residential Ltd | Real estate | 2025 |
| JSC Properties (Borders) Ltd | Real estate | 2014 |
| Juniper Jinn Limited | Manufacturing | 2022 |
| Kapb Limited | Wholesale & retail | 2011 |
| Kelly Powers Ltd | Information & communication | 2023 |
| Kerberos Computing Limited | Information & communication | 2018 |
| Khandallah Ltd | Financial services | 2025 |
| Kib-Build Ltd | Construction | 2022 |
| Kidar Ltd | Professional & scientific | 2018 |
| Kippikirk Limited | Construction | 2024 |
| Kithandwild Ltd | Other services | 2025 |
| Kitty's Travel and Adventures Ltd | Administrative & support | 2024 |
| Lachlan Munro Limited | Administrative & support | 2003 |
| Lagom Assets (UK) Ltd | Wholesale & retail | 2025 |
| Langlands Farrier Services Limited | Agriculture & fishing | 2004 |
| Langside Kennels Ltd | Agriculture & fishing | 2016 |
| LC Squared Ltd | Information & communication | 2023 |
| Leven Brown Adventure Ltd | Manufacturing | 2016 |
| Lilliesleaf Community Development Ltd | Other services | 2018 |
| Live Borders Limited | Arts & recreation | 2003 |
| LMI Consulting Ltd | Professional & scientific | 2024 |
| Lmjb Properties Ltd | Real estate | 2018 |
| LOW Carbon Studio Limited | Professional & scientific | 2010 |
| Lowood Tweedbank Limited | Real estate | 2018 |
| MAC Equestrian Melrose Ltd | Agriculture & fishing | 2026 |
| MAC Horse Transport Ltd | Transport & logistics | 2017 |
| Macvicars of Melrose Ltd | Wholesale & retail | 2003 |
| Malcolm M. Stewart | | 1991 |
| Marmions (Scotland) Company Limited | Hospitality | 2006 |
| Mccran Contractors Limited | Construction | 2016 |
| MCD Financial Limited | Professional & scientific | 2024 |
| Mcgregor Whillans Limited | Real estate | 2002 |