| A & P Sharpe Limited | Professional & scientific | 2017 |
| A Park & Co Limited | Financial services | 2023 |
| Abcons Property Services Ltd | Construction | 2026 |
| Aiop Limited | Information & communication | 2016 |
| Akzfn Limited | Hospitality | 2020 |
| Alba Business Initiative Limited | Professional & scientific | 2016 |
| Alexander Park & SON | Agriculture & fishing | 2000 |
| Allen-Donaldson Ltd | Wholesale & retail | 2024 |
| Andrew KAY & Company (Curling Stones) Limited | Manufacturing | 1958 |
| Aqua Bathrooms (Ayrshire) Limited | Construction | 2018 |
| Aqua Rooms Ltd | Wholesale & retail | 2021 |
| AR Rural Consultancy Services Limited | Professional & scientific | 2017 |
| Archnet Real Estate Ltd | Real estate | 2026 |
| Aries Villa Limited | Other services | 2025 |
| Armour Veterinary Group Ltd | Professional & scientific | 2014 |
| ARRAN15 Limited | Construction | 2025 |
| Art Sales International Ltd | Wholesale & retail | 2024 |
| Atkinson Hislop & Associates Limited | Professional & scientific | 2013 |
| Auchenfail Estates Ltd | Real estate | 2026 |
| Augment Yourself Ltd | Other services | 2018 |
| AWM Plant Hire Ltd | Administrative & support | 2017 |
| Ayrshire Architectural Heritage Trust | Administrative & support | 1991 |
| Ayrshire Building Contracts Ltd | Construction | 2021 |
| Ayrshire Chip 'N' Logs Limited | Energy | 2016 |
| Ayrshire Driveline Services Limited | Wholesale & retail | 2024 |
| Ayrshire Independent Funeral Directors Ltd | Other services | 2026 |
| Ayrshirepropertyoption Ltd | Real estate | 2024 |
| Ballochfield Sporting Dogs Ltd | Agriculture & fishing | 2024 |
| Be.Smart Consulting Ltd | Information & communication | 2024 |
| Blackbull Tarbolton Bar Ltd | Hospitality | 2025 |
| Blackbull Tarbolton Ltd | Hospitality | 2025 |
| Blackstone Analytics Ltd | Professional & scientific | 2020 |
| BOB B Limited | Construction | 2019 |
| Bryson Accountancy Services Ltd | Professional & scientific | 2022 |
| Burnanne Distilling Limited | Manufacturing | 2023 |
| BY Merit Advisory Ltd | Professional & scientific | 2026 |
| C J Roofing Limited | Construction | 2009 |
| Caledonian Drams Ltd | Hospitality | 2025 |
| Cape Good Hope Limited | Extraterritorial | 2003 |
| Cape Horn Property Limited | Real estate | 2001 |
| Catrine Community Renewables Ltd | Energy | 2015 |
| Catrine Community Trust | Financial services | 1992 |
| Catrine PUB Ltd | Hospitality | 2025 |
| CC Roofing Ltd | Construction | 2023 |
| Certigo Ltd | Health & social care | 2026 |
| Chilli Refrigeration Limited | Manufacturing | 1975 |
| CL Commercial Support Ltd | Professional & scientific | 2018 |
| Coilltean ÙRA Forest Services Ltd | Agriculture & fishing | 2025 |
| Compliance LEV Ltd. | Professional & scientific | 2018 |
| Compulearn Business Services Ltd | Administrative & support | 2017 |
| Craighead Nurseries Ltd | Education | 2015 |
| Craigie Street Food Limited | Hospitality | 2025 |
| CW Contracts Ltd | Construction | 2019 |
| D Craig Stonemasonry Ltd | Construction | 2020 |
| D Wilson Lettings Ltd | Real estate | 2026 |
| D&K Haulage Ltd | Transport & logistics | 2025 |
| Dave Metal Ltd | Manufacturing | 2026 |
| Dean Frew Ltd | Wholesale & retail | 2026 |
| Deskline Traders Ltd | Wholesale & retail | 2025 |
| DF Electrical (Ayrshire) Limited | Construction | 2016 |
| Discount Pet Foods Limited | Manufacturing | 2014 |
| DR Rebecca White Aesthetics Ltd | Health & social care | 2020 |
| Ecoheat GAS Services Ltd | Construction | 2015 |
| El-Es Contracts Ltd | Construction | 2016 |
| Euterpia Ltd | Information & communication | 2024 |
| Expert Cleans Ayrshire Limited | Administrative & support | 2023 |
| Factor Cleaning Ltd | Administrative & support | 2018 |
| Faddes Welding & Fabrication Limited | Manufacturing | 2019 |
| Field View Technical Services Ltd | Mining & quarrying | 2013 |
| Fountain Farming Auchinleck | | 1979 |
| Fraoch Ltd | Professional & scientific | 2023 |
| FS Construction Ltd | Construction | 2018 |
| G Roofing Ltd | Construction | 2026 |
| G.F.J.C. Compliance Services Limited | Professional & scientific | 2020 |
| Geisel Consulting Limited | Professional & scientific | 2014 |
| Genesis Rail & Plant UK Ltd | Construction | 2025 |
| GL Property (AYR) Ltd | Real estate | 2026 |
| Gleniffer Holdings Ltd | Financial services | 2025 |
| Glenstang Investments Limited | Other services | 2022 |
| Gljh Property Ltd | Real estate | 2022 |
| Herradura Engineering Services Ltd | Manufacturing | 2018 |
| Hodge Dairies Ltd | Wholesale & retail | 2020 |
| Hodge Developments Ltd | Construction | 2023 |
| Hornbrook Ltd | Hospitality | 2021 |
| Howie Forestry Solutions Ltd | Agriculture & fishing | 2021 |
| Hugh Young Partnership | | 1992 |
| Hunter Sloan Clark Limited | Wholesale & retail | 2022 |
| I DO in Greece Ltd | Professional & scientific | 2026 |
| Imperial Sun Ltd | Other services | 2024 |
| Impraxia Ltd | Financial services | 2024 |
| Indivus (Scotland) Ltd | Water & waste | 2023 |
| Island Roofers Limited | Construction | 2024 |
| Jackall Holdings Limited | Real estate | 2024 |
| James Lawson Building Contractors Ltd | Construction | 2014 |
| JBM Solutions Ayrshire Ltd | Other services | 2025 |
| JD People Co Limited | Administrative & support | 2026 |
| Jennifer Mary Limited | Financial services | 2023 |
| JNG Capital Assets Ltd | Real estate | 2024 |
| JR Homes and Property Ltd | Real estate | 2023 |
| Kevin Clark Services Ltd | Construction | 2023 |