| Kilt & Kaboodle Limited | Wholesale & retail | 2025 |
| Kleboe Contracts Ltd | Manufacturing | 2023 |
| KP Welding Services LLP | | 2011 |
| Ladeside Cottage Ltd | Hospitality | 2022 |
| LF Aero Ltd | Manufacturing | 2025 |
| Liam Martin Ltd | Transport & logistics | 2026 |
| Living Environments Ltd | Construction | 2023 |
| Lochmist Limited | Agriculture & fishing | 2002 |
| Loire Holdings Limited | Real estate | 2024 |
| Lucky House KIM Ltd | Hospitality | 2025 |
| Mani 786 Ltd | Wholesale & retail | 2024 |
| Mansfield House Luxury Lets Ltd | Hospitality | 2025 |
| Mauchline Barbers Ltd | Other services | 2025 |
| Mauchline Burns Bairns Under 5'S Group | Education | 2009 |
| Mauchline Curry House Ltd | Hospitality | 2023 |
| Mauchline Supermarket Ltd | Wholesale & retail | 2024 |
| Maverick Prime Limited | Wholesale & retail | 2024 |
| MBL Property Limited | Real estate | 2019 |
| Medicaid Training Services Ltd | Education | 2024 |
| Messrs James Kennedy Ltd | Agriculture & fishing | 2013 |
| Millar's Groundworks Ltd | Construction | 2020 |
| Miller Steels 2000 Limited | Manufacturing | 2000 |
| Miss Aesthetics Ltd | Other services | 2021 |
| MJ Technology Ltd | Information & communication | 2025 |
| Morgan & Sons Ayrshire Ltd | Administrative & support | 2025 |
| Mossgiel Garage Ltd | Wholesale & retail | 2017 |
| Mossgiel Limited | Manufacturing | 2023 |
| Mossgiel Retail Limited | Wholesale & retail | 2023 |
| MS Plastering Ltd | Construction | 2018 |
| Murdoch Surfacing Ltd | Administrative & support | 2021 |
| Murphy Bathrooms Ltd | Other services | 2022 |
| New Pattern Upskilling Limited | Manufacturing | 2022 |
| Nicolson Property Limited | Real estate | 2019 |
| Nxtgen Academy Scotland Ltd | Education | 2025 |
| Oak Arms Ltd | Wholesale & retail | 2025 |
| Oakburn Property Ltd | Real estate | 2025 |
| Pakee Limited | Wholesale & retail | 2023 |
| Partners in Property Mansfield Ltd | Hospitality | 2025 |
| Partners in Property MANSFIELD2 Ltd | Construction | 2025 |
| Partners in Property Pennyf Ltd | Hospitality | 2025 |
| Pennyfadzeoch Farm Ltd | Hospitality | 2025 |
| Phatanz Ltd | Wholesale & retail | 2024 |
| Plantala Ltd. | Manufacturing | 2021 |
| Precision (Scotland) Limited | Wholesale & retail | 2019 |
| Premier Polymer Solutions Ltd | Manufacturing | 2025 |
| PWM 2023 Ltd | Financial services | 2023 |
| Quality First Construction Ltd | Construction | 2010 |
| Rachel Latchford Dipwcf Equine Services Limited | Agriculture & fishing | 2025 |
| RC Elite Contracts Ltd | Construction | 2021 |
| Renfrewshire Farmers Events Limited | Wholesale & retail | 2025 |
| Reynolds Roofing & Roughcasting Ltd | Construction | 2023 |
| Richwood Home Extensions Ltd | Construction | 2023 |
| Ride on Motorcycles Limited | Wholesale & retail | 1977 |
| River AYR Retreats Ltd | Hospitality | 2025 |
| Riverside Property Investors Ltd | Real estate | 2021 |
| Robert W Howie Ltd | Construction | 2019 |
| Roddenloft Brewery Ltd | Manufacturing | 2014 |
| Roughdyke Farm Ltd | Agriculture & fishing | 2023 |
| RS Leisure Scot Ltd | Hospitality | 2020 |
| RS91 Construction Limited | Construction | 2020 |
| Saltire Wind Solutions Ltd | Manufacturing | 2024 |
| Sandgate Trading Co. Ltd. | Wholesale & retail | 2014 |
| Sanitologic Ltd | Water & waste | 2026 |
| Sasquatch Technologies Limited | Information & communication | 2021 |
| Seaman Properties Limited | Real estate | 2023 |
| SG Access Ltd | Construction | 2026 |
| SGR (AYR) Ltd | Construction | 2025 |
| Sharmaleespirituality Ltd | Other services | 2023 |
| Sharpes Coachworks Ltd | Wholesale & retail | 2024 |
| Shear Subsea Limited | Extraterritorial | 2010 |
| Shearer Tree Services Ltd | Professional & scientific | 2014 |
| Shire Pest and Property Care Ltd | Administrative & support | 2025 |
| Smithfield House Ltd | Hospitality | 2019 |
| SMW Contracts Ltd | Construction | 2016 |
| Sohal Mini Market Ltd | Wholesale & retail | 2024 |
| Solar and Wind Applications Limited | Construction | 2002 |
| Sorn Energy Ltd | Administrative & support | 2011 |
| Sorn Milk Limited | Manufacturing | 1994 |
| Sorn Milk Transport Limited | Administrative & support | 1994 |
| ST Elmo Holdings Ltd | Financial services | 2023 |
| Sultan Street Food Limited | Hospitality | 2025 |
| Sutcliffe Haulage Ltd | Transport & logistics | 2010 |
| Swpca | Other services | 2006 |
| Syke Farm Ltd | Real estate | 2020 |
| Syrians Barbers Ltd | Other services | 2022 |
| Tanner’S Carpets and Flooring Ltd | Wholesale & retail | 2026 |
| Tarbolton Timber Services Ltd. | Construction | 2006 |
| Teal Engineering Services Limited | Manufacturing | 2000 |
| THE Jaggy Nettle Bistro Limited | Hospitality | 2020 |
| Thistlebank Developments Ltd | Real estate | 2020 |
| Timber Technics (Scotland) Ltd. | Construction | 2005 |
| Timber Technics Developments Ltd. | Real estate | 2005 |
| TJS Workx Limited | Wholesale & retail | 2026 |
| Tompkins Property Limited | Real estate | 2025 |
| Top Mark Enterprises Ltd | Hospitality | 2024 |
| Torridon Construction Ltd | Construction | 2025 |
| Totally Natural Limited | Wholesale & retail | 2019 |
| Unlimited Landscapes Ltd | Administrative & support | 2016 |
| Usigorso Limited | Information & communication | 2025 |
| Victoria Rosettes Limited | Manufacturing | 1980 |