| E-VALUE8 (UK) Ltd | Professional & scientific | 2007 |
| E. Ward Limited | Financial services | 1954 |
| East GB Group Limited | Professional & scientific | 2017 |
| East GB Holding Limited | Education | 2022 |
| East GB Limited | Information & communication | 2005 |
| East Midlands Audit Services Ltd | Professional & scientific | 2022 |
| Eastlea Project Management Ltd | Professional & scientific | 2022 |
| Ecclesbourne Valley Railway Association | Administrative & support | 2004 |
| Eco Power Engineering Limited | Manufacturing | 2022 |
| Eco Power Star Design Interiors Limited | Construction | 2022 |
| Eco Shifters Limited | Transport & logistics | 2018 |
| Eco-Bat B.V. | | 2002 |
| Ecosmart Farming Ltd | Agriculture & fishing | 2022 |
| Ecosmart Forestry Ltd | Agriculture & fishing | 2022 |
| Ecosmart Fuels Limited | Wholesale & retail | 2016 |
| Ecosmart Mechanical and Electrical Services Ltd | Extraterritorial | 2017 |
| Eddowes Aviation Safety Limited | Professional & scientific | 2012 |
| Eden Park Investments Limited | Real estate | 2020 |
| Edgelow Events Services Ltd | Water & waste | 2025 |
| Education Personnel Ltd | Administrative & support | 2023 |
| Edward Caudwell & Company Limited | Real estate | 2004 |
| EG Photography Limited | Professional & scientific | 2014 |
| Ego-Design Ltd | Administrative & support | 2010 |
| EJ Interiors (Derby) Ltd | Professional & scientific | 2019 |
| EL Case Management Ltd | Health & social care | 2013 |
| EL Magico Ltd | Construction | 2025 |
| Elate Staffing Solutions Ltd | Administrative & support | 2014 |
| Electrical Systems Limited | Manufacturing | 2001 |
| Electritec Ltd | Construction | 2018 |
| Elelem Artificial Intelligence Limited | Information & communication | 2023 |
| Elevor Limited | Wholesale & retail | 2025 |
| Elfin Treehouse Limited | Arts & recreation | 2025 |
| Elite Fitout U.K. Ltd | Construction | 2017 |
| Elizabeth Malcolm Limited | Extraterritorial | 2017 |
| Elizabeth Norman Ultrasound Ltd | Health & social care | 2026 |
| Elldell Trading Ltd | Wholesale & retail | 2024 |
| Elna Group Limited | Other services | 2020 |
| Elsa Foods Limited | Hospitality | 2026 |
| Elveston Limited | Construction | 2005 |
| Elysium Landscapes Limited | Administrative & support | 2018 |
| Ember Management Accounting Services Ltd | Professional & scientific | 2018 |
| Emery Landscapes Limited | Construction | 1987 |
| EMM Sales and Lettings Limited | Real estate | 2019 |
| Emporium Media Limited | Professional & scientific | 2021 |
| Enfield Sports Ltd | Administrative & support | 2018 |
| Engineering Solutions Today Limited | Professional & scientific | 2024 |
| English Land and Estates Developments Limited | Construction | 2002 |
| Enigma Education Ltd | Administrative & support | 2023 |
| Entire Facilities Management Limited | Administrative & support | 2015 |
| Entswood Limited | Administrative & support | 2011 |
| Enviroclean Derbyshire Ltd | Administrative & support | 2021 |
| EOS Business Consultants Limited | Professional & scientific | 2004 |
| Epic Bars (Andover) Ltd | Hospitality | 2023 |
| Epic Bars (Camberley) Ltd | Hospitality | 2023 |
| EPR Investments Ltd | Real estate | 2021 |
| Eraih Limited | Real estate | 2014 |
| Erskine Hurt Consultants Limited | Professional & scientific | 2001 |
| ESC49E Ltd | Administrative & support | 2025 |
| Escape to Britton Limited | Real estate | 2026 |
| Eseldi Ltd | Professional & scientific | 2020 |
| Eternal Holdings Limited | Real estate | 2023 |
| Evans Edwards Jewellers Ltd | Wholesale & retail | 2023 |
| Evans Vettori Architects Limited | Professional & scientific | 2004 |
| Everad John Ltd | Professional & scientific | 2019 |
| Evre Bike Limited | Wholesale & retail | 2011 |
| Evre Services Limited | Administrative & support | 2022 |
| Exhaust Products Limited | Manufacturing | 2012 |
| Exile Music Festival Ltd | Arts & recreation | 2019 |
| Eyeview Properties Limited | Real estate | 2024 |
| Fantastic Foodies Ltd | Hospitality | 2022 |
| Farmhouse Brands Ltd | Professional & scientific | 2013 |
| Fast Fuelled Ltd | Health & social care | 2023 |
| Faulkner Media Solutions Limited | Manufacturing | 2013 |
| FED by NED Ltd | Hospitality | 2024 |
| Feel Good DO Good Limited | Administrative & support | 2014 |
| Felix BUS Services Limited | Real estate | 1975 |
| Festival Safeguarding Ltd | Public administration | 2024 |
| FEW4 Limited | Real estate | 2016 |
| Fewkes Huff Management Limited | Other services | 2024 |
| Fewkes Sport Management Limited | Other services | 2017 |
| Fidler Taylor Ltd | Real estate | 2013 |
| Film Wifi Limited | Information & communication | 2017 |
| Filmwifi International Ltd | Administrative & support | 2025 |
| Fiona Filby Wellbeing Ltd | Other services | 2026 |
| Fire Integrity North Limited | Construction | 2024 |
| Fire Integrity Special Projects Limited | Construction | 2023 |
| Fire Safe Professionals Limited | Information & communication | 2018 |
| Firm Property Management (Derbyshire) Ltd | Households | 2025 |
| Fitting Gifts Limited | Wholesale & retail | 2011 |
| Fitzpatrick Consultancy Services Limited | Professional & scientific | 2024 |
| FKE Group Ltd | Construction | 2018 |
| Flare Design and Consultancy Ltd | Information & communication | 2001 |
| Fletcher Property Projects Limited | Real estate | 2019 |
| FLOGIT4U Ltd | Wholesale & retail | 2017 |
| Florence Nightingale Suites Ltd | Hospitality | 2020 |
| Florrie Farmers Limited | Professional & scientific | 2015 |
| Flux Capacitor Technologies Ltd | Information & communication | 2019 |
| Flydar Ltd | Professional & scientific | 2024 |
| FML Education Consultancy Limited | Education | 2012 |
| Food to Fit Limited | Professional & scientific | 2014 |