| THE Moot Oak Brewing Company Limited | Manufacturing | 2017 |
| THE Moot Oak Ltd | Hospitality | 2017 |
| THE Musical ME Ltd | Education | 2020 |
| THE Nelson Arms Middleton Limited | Hospitality | 2012 |
| THE Old Blacksmiths Ltd | Real estate | 2019 |
| THE Old Lock UP Gallery Ltd | Arts & recreation | 2017 |
| THE Orangery Cromford Ltd | Hospitality | 2025 |
| THE OWL Shed Limited | Professional & scientific | 2012 |
| THE Peak Building Brigade Limited | Construction | 2017 |
| THE Penny Brick Company Limited | Manufacturing | 2025 |
| THE Playmill Limited | Arts & recreation | 2016 |
| THE Qlinic Ltd | Health & social care | 2022 |
| THE Quarryman 2025 Ltd | Hospitality | 2025 |
| THE Red Lion Hotel (Wirksworth) Ltd | Wholesale & retail | 2019 |
| THE Red Tent - Studio of the Sacred Ltd | Arts & recreation | 2025 |
| THE Remarkable Hare Limited | Hospitality | 2019 |
| THE Ringsfield Hall Trust | Education | 2001 |
| THE Salon Wirksworth Limited | Wholesale & retail | 2014 |
| THE Self Guided Mountain Bike Company Ltd | Administrative & support | 2023 |
| THE Shalimar (Matlock) Limited | Hospitality | 2003 |
| THE SIR William Derbyshire Ltd | Hospitality | 2025 |
| THE Social Media Group Limited | Professional & scientific | 2026 |
| THE Standardbred and Trotting Horse Association of Great Britain and Ireland | Agriculture & fishing | 1998 |
| THE Stone Soup Project Community Interest Company | Hospitality | 2007 |
| THE Typeface (GRP) Ltd | Other services | 2012 |
| THE Walrus and the Carpenter Limited | Real estate | 2023 |
| THE Welcome Team Limited | Administrative & support | 2020 |
| THE Whitworth Centre | Arts & recreation | 2007 |
| THE Wirksworth Pottery Company Ltd | Manufacturing | 2021 |
| THE Woodlands Fitness Centre Limited | Arts & recreation | 1999 |
| Thea Systems Ltd | Information & communication | 2025 |
| Thinkingitbetter Limited | Professional & scientific | 2011 |
| Thompson Hospitality Limited | Hospitality | 2021 |
| Thompson Tree Services (Midlands) Ltd | Extraterritorial | 2023 |
| Thompson Tree Services (UK) Ltd | Agriculture & fishing | 2014 |
| Thompsons Group Ltd | Administrative & support | 2020 |
| Thought Studio Limited | Information & communication | 2010 |
| Three Peaks Roofing Limited | Construction | 2026 |
| Three Pillar Homes Limited | Construction | 2025 |
| Three Stags Darley Bridge Ltd | Hospitality | 2023 |
| Tickgood Ltd | Hospitality | 2024 |
| Tieg Creations Ltd | Professional & scientific | 2024 |
| Tinker's Place Management Company Limited | Real estate | 2008 |
| Tisbury Business Services Limited | Administrative & support | 2005 |
| Tissington Court Management Company Limited | Households | 2008 |
| TJS67 Ltd | Construction | 2024 |
| Toll Bar Cottage Limited | Hospitality | 2018 |
| Tonetix Limited | Administrative & support | 2020 |
| Top Cutz Barbers 1 Ltd | Other services | 2023 |
| TOR Electrical (Matlock) Limited | Construction | 2001 |
| TOR Studio Limited | Information & communication | 2006 |
| Tostca's Limited | Hospitality | 2013 |
| Town Hall Events Wirksworth CIC | Arts & recreation | 2025 |
| Trackback Solutions Limited | Professional & scientific | 2025 |
| Trackhunter Limited | Information & communication | 2013 |
| Trades Box Ltd | Financial services | 2023 |
| Treacle Properties Ltd | Construction | 2015 |
| Tredici Limited | Hospitality | 2021 |
| Trendical Holdings Ltd | Information & communication | 2016 |
| Trevor Page Consultancy Ltd | Professional & scientific | 2024 |
| Trick Bits Limited | Manufacturing | 2007 |
| Tsjp Ltd | Professional & scientific | 2013 |
| Turner Building Contractors Limited | Construction | 2005 |
| Turners Graphic Art and Drawing Office Supplies Ltd. | Information & communication | 2005 |
| TWM Development Limited | Construction | 2021 |
| Two Dales News Limited | Wholesale & retail | 2010 |
| Twyford James Ltd | Wholesale & retail | 2023 |
| U.K. Slipform Limited | Construction | 2007 |
| UDS Holdco Limited | Financial services | 2023 |
| Ugly Duckling Designs Limited | Professional & scientific | 2025 |
| UK Bike Fit Ltd | Wholesale & retail | 2021 |
| UKS Holdings Limited | Construction | 2021 |
| Uminet Limited | Hospitality | 2023 |
| Uncommon Aspect LLP | | 2023 |
| Underhall Respite and Resource Centre Ltd | Health & social care | 2017 |
| Upperwood Limited | Other services | 2006 |
| UPS Holdings Limited | Financial services | 2024 |
| UPS Power Services Ltd | Administrative & support | 2017 |
| Urban Construct Limited | Construction | 2003 |
| Utilitec Limited | Professional & scientific | 1982 |
| Utilitec Services Limited | Professional & scientific | 1997 |
| Utility Data Services Limited | Information & communication | 1998 |
| UX1 Ltd | Information & communication | 2014 |
| V W R Builders Limited | Construction | 2005 |
| Valley Carpets Chesterfield Limited | Wholesale & retail | 2022 |
| Vapourtrail Systems Ltd | Information & communication | 2013 |
| Vaults Property Company Limited | Households | 2015 |
| Ve-Bakes Ltd | Extraterritorial | 2019 |
| Vecor Ltd | Wholesale & retail | 2023 |
| VEE Electrical Services Ltd | Construction | 2024 |
| Venture LEA Design Ltd | Administrative & support | 2025 |
| Veromatt Ltd | Hospitality | 2025 |
| Vetech Product Design and Development Ltd | Professional & scientific | 1994 |
| VIA Gellia Investment Company (the) | Extraterritorial | 1970 |
| Vibo Trading 2 Limited | Hospitality | 2015 |
| Vibo Trading Ltd | Hospitality | 2015 |
| Videc Lamb Estates Ltd | Real estate | 2025 |
| Videc Properties Ltd | Real estate | 2023 |
| Vita Design Ltd | Professional & scientific | 2018 |
| Vitalforce Limited | Households | 1983 |