| Structure and Thermal Ltd | Professional & scientific | 2017 |
| Studio 55 Limited | Transport & logistics | 1991 |
| Sturdybydesign Limited | Wholesale & retail | 2017 |
| STW Maintenance Ltd | Wholesale & retail | 2019 |
| Suffolk 74 Limited | Professional & scientific | 2025 |
| Sunset Barn Interiors Ltd. | Wholesale & retail | 2016 |
| Supercar Group Ltd | Wholesale & retail | 2024 |
| Supply Lines Limited | Wholesale & retail | 2001 |
| Surf's UP Holidays Ltd | Hospitality | 2025 |
| Swanbarton Limited | Professional & scientific | 2003 |
| Sweetland Construction and Management Ltd | Professional & scientific | 2005 |
| Sweetnam & Bradley Limited | Manufacturing | 2008 |
| Syntegrate Ltd. | Manufacturing | 2017 |
| Systemfender Consulting Limited | Construction | 2008 |
| T.R Auto Servicing & Repairs Ltd | Wholesale & retail | 2020 |
| T.R Performance Ltd | Wholesale & retail | 2025 |
| TA Food Ltd | Hospitality | 2026 |
| Tails of Success Ltd | Education | 2019 |
| Talis Legal Recruitment (Regions) LLP | | 2024 |
| Tara the Celebrant Ltd | Other services | 2024 |
| Teague and Capital Limited | Professional & scientific | 1998 |
| Tealmark Ltd | Professional & scientific | 2025 |
| Tech DD Ltd | Professional & scientific | 2014 |
| Tellings Orchard Management Company Limited | Real estate | 1999 |
| Tent to BE Limited | Wholesale & retail | 2024 |
| TF Management Ltd | Real estate | 2018 |
| Tgka Brands Ltd | Wholesale & retail | 2020 |
| THE Active Strategist Limited | Professional & scientific | 2014 |
| THE Beeches Care Training Solutions Limited | Education | 2018 |
| THE Beeches Homecare Services Limited | Health & social care | 2010 |
| THE Bridge Indian Ltd | Hospitality | 2020 |
| THE Butterfly Gardener Limited | Professional & scientific | 2012 |
| THE Calm Clinic Limited | Other services | 2025 |
| THE Cartmell Community Minibus Limited | Transport & logistics | 1999 |
| THE Chimney Sweeper Ltd | Administrative & support | 2017 |
| THE Cotswold Collection 4X4 Ltd | Wholesale & retail | 2026 |
| THE Cotswold Events Company Ltd | Administrative & support | 2016 |
| THE Cotswold Kids Shoe Company Limited | Wholesale & retail | 2017 |
| THE Cotswolds MUM Ltd | Professional & scientific | 2026 |
| THE Daring DO Limited | Professional & scientific | 2000 |
| THE Dispute Clinic Limited | Professional & scientific | 2021 |
| THE Douche BAG Limited | Manufacturing | 2025 |
| THE Dragon Snake Company Limited | Administrative & support | 2009 |
| THE George Group Limited | Professional & scientific | 2015 |
| THE Human Diver Ltd | Professional & scientific | 2019 |
| THE Humaneers Ltd | Professional & scientific | 2020 |
| THE Iron Works and Gate Company Limited | Manufacturing | 2012 |
| THE KA Coaching Inn Limited | Hospitality | 2019 |
| THE Last Baguette Theatre Company CIC | Arts & recreation | 2020 |
| THE Light (Malmesbury) Management Co. Ltd | Households | 2012 |
| THE Luxury Safari Company Ltd | Administrative & support | 2010 |
| THE Malmesbury Cider Company Ltd | Manufacturing | 2016 |
| THE Malmesbury Clinic Ltd | Health & social care | 2018 |
| THE Malmesbury Wine Company Ltd | Wholesale & retail | 2025 |
| THE Moscow State Circus Limited | Arts & recreation | 1991 |
| THE Natural Face Aesthetics Group Ltd | Administrative & support | 2017 |
| THE Old Coach Yard Limited | Hospitality | 2015 |
| THE Original Book Works Limited | Manufacturing | 1992 |
| THE Presentation Coach Ltd | Professional & scientific | 2017 |
| THE Purposeful Coach Limited | Extraterritorial | 2020 |
| THE Ramona Flowers LLP | | 2016 |
| THE Royal Flush Limited | Water & waste | 2003 |
| THE Show Will Go on Ltd | Arts & recreation | 2020 |
| THE Space at 2A Ltd | Health & social care | 2025 |
| THE Spinney (Malmesbury) Management Company Limited | Households | 2020 |
| THE Stay Safe Initiative CIC | Education | 2019 |
| THE Timber Extension Company Ltd | Construction | 2023 |
| THE Tomato LAB Ltd | Agriculture & fishing | 2025 |
| THE Tracklement Company Limited | Manufacturing | 1979 |
| THE Wiltshire Sausage Company Ltd | Manufacturing | 2025 |
| Themalmesburyclinic Ltd | Health & social care | 2026 |
| Thirty SIX Consulting Limited | Professional & scientific | 2022 |
| Thomas Knights Studio Ltd | Arts & recreation | 2003 |
| Thomas of Malmesbury Limited | Hospitality | 2006 |
| Thrive Ability Ltd | Health & social care | 2025 |
| Thunderbird Natural Skincare Limited | Wholesale & retail | 2024 |
| Tietoa Limited | Health & social care | 2015 |
| Tigerplay Airworks Limited | Manufacturing | 2009 |
| Tigerplay Cafe Limited | Hospitality | 2019 |
| Till WE Meet Again Ltd | Professional & scientific | 2025 |
| Tinplate Products Limited | Wholesale & retail | 2002 |
| Titan Incubators Limited | Extraterritorial | 2014 |
| TLC Property Limited | Real estate | 2008 |
| TNC Surfacing Ltd | Construction | 2023 |
| Toff Tails Ltd | Wholesale & retail | 2025 |
| TOM Grant Eventing Ltd | Other services | 2017 |
| Tony Bolwell & SON Limited | Other services | 1990 |
| Top Notch Rooms Limited | Real estate | 2006 |
| Town & Country Property Services Ltd | Real estate | 2017 |
| Trecelyn Limited | Professional & scientific | 2003 |
| TRM Process Solutions Ltd | Professional & scientific | 2025 |
| Twentyfirst Properties Ltd | Real estate | 2020 |
| Ujamaflip Limited | Agriculture & fishing | 2017 |
| UMF Storage Limited | Transport & logistics | 2015 |
| Unbarrier Education Ltd | Information & communication | 2025 |
| Unitec Design & Construction (Engineering) Limited | Construction | 1988 |
| V Pike Builders Limited | Construction | 2011 |
| V&E Baths Ltd | Wholesale & retail | 2024 |
| Vector Support Solutions Limited | Public administration | 2025 |
| Verdigris Consulting Limited | Information & communication | 2017 |