| Rb-Mtec Limited | Manufacturing | 2024 |
| REA Landscaping Ltd | Administrative & support | 2026 |
| Real Power Consulting Ltd | Professional & scientific | 2024 |
| Red Duster Limited | Administrative & support | 2013 |
| Red Hot 100 Ltd | Arts & recreation | 2011 |
| Red Kite Helicopters Ltd | Manufacturing | 2011 |
| Redhouse Analytics (Wiltshire) Ltd | Professional & scientific | 2017 |
| Ree-Invent Beauty Limited | Other services | 2023 |
| Reflex Medical Limited | Wholesale & retail | 2019 |
| Regency Apartments Management Company Limited | Households | 2015 |
| Reiki Your Future Ltd | Education | 2025 |
| Relative Agency Limited | Professional & scientific | 2011 |
| Relatr Ltd | Professional & scientific | 2018 |
| Renosity Limited | Manufacturing | 2025 |
| RENOV8 EPM Ltd | Real estate | 2019 |
| Reset Physiotherapy and Wellness Limited | Other services | 2024 |
| Return to Sender Limited | Wholesale & retail | 2005 |
| Rgsv Limited | Other services | 2022 |
| Rheign Limited | Wholesale & retail | 2012 |
| Richard Knight Bloodstock Agent Ltd | Agriculture & fishing | 2014 |
| Riches Interiors Ltd | Professional & scientific | 2022 |
| Right Choice Group Limited | Wholesale & retail | 2023 |
| Rippleworks Limited | Information & communication | 2025 |
| RJI Projects Ltd | Administrative & support | 2007 |
| RK Surveyors Limited | Professional & scientific | 2013 |
| RL Propco Limited | Real estate | 2024 |
| Rocking BEE Limited | Administrative & support | 2017 |
| Rocking Zebra Limited | Administrative & support | 2009 |
| Roller Bird Ltd | Professional & scientific | 2012 |
| Roost Restaurant & Bar Limited | Hospitality | 2023 |
| Root & Branch Global LLP | | 2016 |
| Rosemond Associates Ltd | Professional & scientific | 2018 |
| RS Woodworks Ltd | Manufacturing | 2013 |
| Rumbawly Limited | Health & social care | 2009 |
| Rushcroft (LEA) Management Limited | Households | 1999 |
| Rushmoore Ltd | Administrative & support | 2017 |
| Russell House Construction Limited | Construction | 2024 |
| Russell House Holdings Limited | Construction | 2026 |
| RWD Concepts Ltd | Administrative & support | 2021 |
| S & K Digital Limited | Manufacturing | 2001 |
| S & R Fruits Limited | Wholesale & retail | 2003 |
| S&P Event Medical and Training Ltd | Education | 2024 |
| Salient Enterprises Limited | Information & communication | 2011 |
| Sapporo Services Ltd | Construction | 2019 |
| Sarah Grace Mortgages Limited | Financial services | 2015 |
| Saxbyvirtualadministrators Ltd | Administrative & support | 2026 |
| Saxon House (Malmesbury) Management Limited | Administrative & support | 1988 |
| SBA Cedar Limited | Real estate | 2026 |
| Scaling the Heights LLP | | 2014 |
| Schramm Creative Ltd | Construction | 2016 |
| Scooter Works Ltd | Wholesale & retail | 2020 |
| Scorribanda Holdings Limited | Professional & scientific | 2002 |
| Seager Welding Ltd. | Manufacturing | 2005 |
| Service Company One Limited | Agriculture & fishing | 2019 |
| Servizio Workshop Limited | Manufacturing | 2024 |
| Settlementagreementsuk Limited | Professional & scientific | 2026 |
| SG South West Ltd | Construction | 2018 |
| Sharpes Windows & Doors Ltd | Construction | 2015 |
| Shaw About Wine Ltd | Wholesale & retail | 2024 |
| Shaw Advisory Partners Ltd | Information & communication | 2026 |
| Shelina Gofur Consulting Limited | Professional & scientific | 2025 |
| Shelton Plant Limited | Wholesale & retail | 2007 |
| Sherston Boutique Limited | Other services | 2023 |
| Sherston Doggy Daycare Ltd | Agriculture & fishing | 2021 |
| SHIP2SHORE Engineering Limited | Administrative & support | 2018 |
| Sillars Management Consulting Ltd | Professional & scientific | 2018 |
| Silverchart Systems Limited | Information & communication | 1996 |
| Simon Bristow Associates Limited | Construction | 2018 |
| Simon Gower Services Limited | Professional & scientific | 2008 |
| Simon Locke Designs Limited | Manufacturing | 2019 |
| Simply Bespoke South West Limited | Manufacturing | 2007 |
| Simply Motor Events Limited | Administrative & support | 2025 |
| Simpson & Partners (EV) Ltd | Manufacturing | 2024 |
| Smart My Car Ltd | Wholesale & retail | 2021 |
| Smith & Statton Limited | Transport & logistics | 2024 |
| SN Windscreen Repair Ltd | Wholesale & retail | 2025 |
| Snipper Nixon Limited | Information & communication | 2005 |
| Snug Living Limited | Wholesale & retail | 2000 |
| Solar GEO Limited | Construction | 2009 |
| Soulagent Ltd | Extraterritorial | 2015 |
| South Cotswold Properties Ltd | Real estate | 2024 |
| South West Solar Parks Limited | Professional & scientific | 2011 |
| Southern Airframe Services Ltd | Professional & scientific | 2011 |
| Specialist Medical Sourcing Group Limited | Wholesale & retail | 2024 |
| Springs of Grace Limited | Public administration | 2025 |
| Squire of Sherston Limited | Real estate | 2006 |
| Stamp Duty Filing Limited | Professional & scientific | 2026 |
| Starke Creative Limited | Professional & scientific | 2014 |
| Steel Design Solutions Limited | Professional & scientific | 2000 |
| Stencil Shed Ltd | Wholesale & retail | 2003 |
| Stenson Swimming LLP | | 2013 |
| Stepcraft Service Hub (UK) Limited | Manufacturing | 2025 |
| Steve Osborne Carpentry and Construction Ltd | Construction | 2026 |
| Steve Osborne Construction Ltd | Construction | 2021 |
| Steven Dunn | | 1997 |
| Steves Films Ltd | Information & communication | 2025 |
| Stocks Electrical Limited | Construction | 2024 |
| Storey Mews (Malmesbury) Management Company Limited | Households | 2014 |
| Strawberry Lane Consulting Limited | Professional & scientific | 2023 |
| Strawberry Lane Limited | Professional & scientific | 2023 |