| Ellis Payments Ltd | Other services | 2022 |
| Elms Marketing Limited | Wholesale & retail | 2000 |
| Elov Community C.I.C. | Manufacturing | 2024 |
| Elucitec UK Limited | Professional & scientific | 2020 |
| Elvaé Limited | Professional & scientific | 2025 |
| Elysian Consult Ltd | Professional & scientific | 2024 |
| Elysian Hair Studio Ltd | Other services | 2024 |
| Elysium Beauty Consultants Limited | Other services | 2007 |
| EM Analytical Ltd | Professional & scientific | 2018 |
| EM Resist Limited | Manufacturing | 2011 |
| EM Scientific Limited | Professional & scientific | 2017 |
| EM Systems Support Limited | Manufacturing | 2002 |
| Email Made Easy Limited | Administrative & support | 2025 |
| Emar Bars Ltd | Hospitality | 2023 |
| Emar Pack Ltd | Hospitality | 2024 |
| EMB Advanced Technologies Limited | Manufacturing | 2013 |
| Emblazed Ltd | Professional & scientific | 2012 |
| Emchiro Limited | Health & social care | 2024 |
| Emerson Industries Limited | Information & communication | 2025 |
| Emery Planning Group Limited | Financial services | 2021 |
| Emery Planning Holdings Limited | Financial services | 2016 |
| Emery Planning Partnership Limited | Professional & scientific | 2002 |
| EMF Shelter Ltd | Professional & scientific | 2015 |
| Emma Cheetham Consulting Limited | Professional & scientific | 2024 |
| Emma Lucy Ltd | Real estate | 2018 |
| Emmo Holdings Ltd | Financial services | 2020 |
| Emoneydirect Ltd | Financial services | 2008 |
| Emoneyhub Limited | Information & communication | 2012 |
| Empirical Ventures BBI Nominees Limited | Other services | 2023 |
| Empower Psychology Services Limited | Education | 2022 |
| Empress Court Property Management Limited | Households | 2026 |
| Emre Food 1 Ltd | Hospitality | 2026 |
| EMS Emea Ltd | Administrative & support | 2012 |
| EMS Group Solutions Ltd | Professional & scientific | 2021 |
| EMS Healthcare Limited | Administrative & support | 2013 |
| EMS Production Ltd | Manufacturing | 2017 |
| Emsee Limited | Administrative & support | 2022 |
| Emwrite Ltd | Professional & scientific | 2017 |
| Enatex Limited | Health & social care | 2009 |
| Enchanted Homes Ltd | Real estate | 2018 |
| Encore Recruitment Limited | Administrative & support | 2020 |
| Endee Limited | Manufacturing | 2002 |
| Endon Quarry Limited | Real estate | 2026 |
| Endopulse Medical Ltd | Health & social care | 2026 |
| Energea Holdings Ltd | Construction | 2013 |
| Energicity Limited | Manufacturing | 2013 |
| Energies Development Solutions Ltd | Administrative & support | 2024 |
| Energy Efficiency Consultancy Group Limited | Financial services | 2013 |
| Energy Efficiency Consultancy Ltd | Construction | 2006 |
| Enfield Court RTM Company Limited | Households | 2018 |
| Engage Your People Limited | Information & communication | 2018 |
| Engineered Communications Solutions (ECS) Ltd | Wholesale & retail | 1998 |
| Enhance Lives Ltd | Health & social care | 2022 |
| ENIGMA2059 Limited | Information & communication | 2021 |
| Enlabels Ltd | Manufacturing | 2018 |
| ENN Chem Pharma Consultancy Limited | Professional & scientific | 2025 |
| Enoss Limited | Professional & scientific | 2012 |
| Enpeople Limited | Administrative & support | 2006 |
| Enquantum Ltd | Information & communication | 2024 |
| Ensemble Social Work Ltd | Health & social care | 2024 |
| Enser Management Consultancy Ltd | Professional & scientific | 2023 |
| Enterprise Property Investment Group Limited | Construction | 2024 |
| Enterprising People Ltd | Administrative & support | 2025 |
| Entertaining Minds Ltd | Arts & recreation | 2019 |
| Entwisle Project Management Ltd. | Professional & scientific | 2014 |
| Enviro Estates Global Limited | Professional & scientific | 2015 |
| Environment Journal Limited | Professional & scientific | 2015 |
| Environmental Biotech Limited | Extraterritorial | 2006 |
| Envirosafe PPE Ltd | Manufacturing | 2020 |
| Enzyme Communications Ltd | Professional & scientific | 2017 |
| EOS Health and Safety Consultancy Ltd | Professional & scientific | 2023 |
| Epic Draws Ltd | Administrative & support | 2021 |
| Epicerie Ludo Limited | Wholesale & retail | 2011 |
| Epona BIO Limited | Professional & scientific | 2025 |
| Equestyle Limited | Wholesale & retail | 2016 |
| Equilibrium Digital Limited | Professional & scientific | 2022 |
| Equilibrium Healthcare Holdings Ltd | Health & social care | 2024 |
| Equipment Rentals and Finance Limited | Financial services | 2013 |
| Equitivo Limited | Extraterritorial | 2018 |
| ER & BE Ltd | Hospitality | 2025 |
| ERE Consulting Ltd | Professional & scientific | 2025 |
| Eriana Ltd | Wholesale & retail | 2023 |
| Eric Bowyer & SON Ltd | Professional & scientific | 2019 |
| Erica Jane Roebuck Limited | Wholesale & retail | 2022 |
| ERT Developments Ltd | Real estate | 2020 |
| ES Housing Limited | Construction | 2020 |
| Escape Garden Design Ltd | Administrative & support | 2023 |
| Escape Spa Ltd | Arts & recreation | 2017 |
| ESM Fire Ltd | Professional & scientific | 2025 |
| Espressions of Love Limited | Wholesale & retail | 2025 |
| Essential Commerce Solutions Ltd | Wholesale & retail | 2026 |
| Essential Solutions Ltd | Construction | 2026 |
| Essential Trade Solutions Ltd | Wholesale & retail | 2025 |
| Essentials Consulting Limited | Administrative & support | 2021 |
| Establish Property Maintenance Ltd | Construction | 2024 |
| Estuary Residential Care Ltd | Health & social care | 2024 |
| Estuary Residential Holdings Ltd | Financial services | 2024 |
| ETA Digital Ltd | Professional & scientific | 2022 |
| ETA Freedom Ltd | Real estate | 2021 |
| ETA Homes Ltd | Real estate | 2023 |