| Enduracore Health Limited | Health & social care | 2025 |
| Enduracore X Limited | Wholesale & retail | 2025 |
| English R Limited | Wholesale & retail | 2025 |
| Enlan Limited | Administrative & support | 2006 |
| Enterprise IT Solutions Ltd. | Information & communication | 2023 |
| Escape Goat Racing Limited | Information & communication | 2023 |
| Eskimarket (Coleford) Limited | Real estate | 1978 |
| Esmanur Ltd | Hospitality | 2016 |
| Estimate & QS Solutions Limited | Professional & scientific | 2014 |
| Ethical Property Management Limited | Real estate | 2013 |
| Etloe Escapes Limited | Real estate | 2025 |
| European Plasma Technology Ltd | Manufacturing | 2003 |
| Eventiously Ltd | Arts & recreation | 2025 |
| Evergreen Social Enterprise C.I.C. | Construction | 1993 |
| Evetts Robinson Atelier Ltd | Professional & scientific | 2025 |
| Evhiz Travel Limited | Administrative & support | 2024 |
| Excellence in Quality Limited | Construction | 2019 |
| Expand the Brand Ltd | Professional & scientific | 2021 |
| Expresser Serviced Facilities Ltd | Real estate | 2020 |
| F.C. Larkham & SON Limited | Construction | 1986 |
| F.W. Johnson Limited | Construction | 1964 |
| Fable & Verse Ltd | Information & communication | 2017 |
| Farthings Estate Agents Limited | Real estate | 2012 |
| FDH Ltd | Professional & scientific | 1992 |
| Ferrino and Partners Limited | Real estate | 2012 |
| Ferrino Properties Limited | Real estate | 2017 |
| Festi Ltd | Information & communication | 2026 |
| Finacraft Limited | Administrative & support | 1995 |
| Financial International Gateway Limited | Information & communication | 2024 |
| Finprosol Limited | Administrative & support | 2025 |
| Finula Ltd | Real estate | 2024 |
| Firestyles (SW) Ltd | Construction | 2021 |
| First Finance (Bristol) Limited | Financial services | 1987 |
| First Stop Stationers Limited | Wholesale & retail | 2009 |
| FIX Auto Bath Limited | Wholesale & retail | 2026 |
| Fleetfix Engineering Ltd | Wholesale & retail | 2025 |
| Food Allergy Friends Limited | Hospitality | 2021 |
| Food, Events and Things (Feat) Limited | Other services | 2009 |
| Footprint Media Limited | Information & communication | 2023 |
| Forecourt Maintenance Services Ltd | Professional & scientific | 2004 |
| Forest Community Transport Limited | Transport & logistics | 2011 |
| Forest Custom and Classics Ltd | Wholesale & retail | 2016 |
| Forest Hotels Limited | Hospitality | 1997 |
| Forest Oak Farm Ltd. | Hospitality | 2009 |
| Forest of Dean Caravans Limited | Transport & logistics | 1984 |
| Forest of Dean Developments Ltd | Hospitality | 2019 |
| Forest of Dean Motor Club Limited | Arts & recreation | 1970 |
| Forest of Dean Plumbing & Heating Ltd | Construction | 2019 |
| Forest of Dean Railway Limited | Transport & logistics | 1983 |
| Forest of Dean Serviced Offices Limited | Real estate | 2019 |
| Forest of Dean Serviced Rooms Ltd | Real estate | 2020 |
| Forest of Dean Valeting Services Limited | Wholesale & retail | 2019 |
| Forest Press Hydraulics Limited | Manufacturing | 1984 |
| Forest Retrofit Ltd | Construction | 2022 |
| Forest Stoneyard Limited | Mining & quarrying | 2020 |
| Forever Foods Limited | Wholesale & retail | 1999 |
| Forever Golf Ltd | Arts & recreation | 2005 |
| Forever Hotel Ltd | Arts & recreation | 2005 |
| Forevergreen Ltd | Agriculture & fishing | 2002 |
| Forge Security Services Limited | Administrative & support | 2002 |
| Fortitude Health and Performance Limited | Arts & recreation | 2016 |
| Fosforos Ltd | Wholesale & retail | 2021 |
| Foxwell Contractors Limited | Construction | 2018 |
| Frenzinet Limited | Information & communication | 2004 |
| Freya Parkhouse Ltd | Professional & scientific | 2024 |
| Future Skin Limited | Other services | 2015 |
| Fynn Aston Ltd | Wholesale & retail | 2025 |
| G L J Gomery Limited | Wholesale & retail | 2014 |
| G8 (UK) Limited | Professional & scientific | 2013 |
| Galleons LAP Films Limited | Information & communication | 2015 |
| Galogjisten Limited | Wholesale & retail | 2024 |
| Gamber Holdings Limited | Real estate | 2018 |
| Gamber Limited | Wholesale & retail | 2023 |
| Gamber Poultry Litter Limited | Wholesale & retail | 2023 |
| Garrison Holdings Limited | Other services | 2018 |
| Gellygen Limited | Real estate | 2000 |
| Geoled Ltd | Construction | 2015 |
| Geoson Limited | Professional & scientific | 2022 |
| Gerbera Management Company Limited | Households | 2006 |
| GFS George Flooring Services Ltd | Construction | 2026 |
| Giois Barbers Ltd | Other services | 2025 |
| Glamorgan Star Ltd | Information & communication | 2021 |
| Gleed Services Limited | Construction | 2000 |
| Glengarry Joinery Limited | Construction | 2022 |
| Global Partnership Assets Ltd | Construction | 2012 |
| Gloucester Mini MIX Construction Limited | Construction | 2004 |
| Gloucestershire Maternity Action Group CIC | Other services | 2026 |
| Glyn Van Poetsch Limited | Transport & logistics | 2019 |
| Goodfoodsource Ltd | Hospitality | 2023 |
| Gordon Gambling Building Consultancy Ltd | Professional & scientific | 2012 |
| Graham Bryce Landscape Architects Ltd | Professional & scientific | 2024 |
| Grazing Management Ltd | Agriculture & fishing | 2021 |
| Great Scott's Fruit and VEG Limited | Wholesale & retail | 2025 |
| Greenacres Parkhill Ltd | Hospitality | 2020 |
| Greenkey Garden & Home Ltd | Wholesale & retail | 2002 |
| Greenman Maintenance Limited | Administrative & support | 2017 |
| Greenside Landscaping Limited | Administrative & support | 2014 |
| Groupfusion Ltd | Real estate | 2025 |
| GWG Mortgage and Finance Limited | Financial services | 2023 |
| H&H Events Limited | Arts & recreation | 2009 |