| CJP (Chris Jenkins Property) Limited | Construction | 2023 |
| Clanna Country Homes Limited | Hospitality | 2021 |
| Classic Cotswold Homes Limited | Construction | 2010 |
| Clavelshay Construction Ltd | Construction | 2019 |
| Clays Wood Reclamation Limited | Water & waste | 1980 |
| Clear & Complete Accountancy Solutions Ltd | Professional & scientific | 2023 |
| Clearway Conservatories Ltd | Construction | 2008 |
| CMF Heating Ltd | Construction | 2026 |
| Coach Nikos Ltd | Real estate | 2021 |
| Coats of Perfection Limited | Construction | 2021 |
| Coinros Park Nurseries Limited | Agriculture & fishing | 2001 |
| Cole Construction Management Ltd | Construction | 2020 |
| Cole Consultancy & Maintenance Ltd | Professional & scientific | 2023 |
| Coleford Electrical Services Limited | Construction | 2012 |
| Coleford MOT Centre Limited | Wholesale & retail | 2025 |
| Coleyco Limited | Information & communication | 2018 |
| Colosseum Fitness Limited | Arts & recreation | 2022 |
| Come & Play Day Nursery Ltd | Education | 2020 |
| Conkers Corner Ltd | Health & social care | 2025 |
| Contec Ltd | Professional & scientific | 2022 |
| Coombs Developments Limited | Construction | 2005 |
| Coombs Property Services Limited | Construction | 2007 |
| Cork Matters Limited | Information & communication | 2013 |
| Cornerways Management Consultancy Limited | Professional & scientific | 2016 |
| Cotswold Energy Consultants Limited | Other services | 2007 |
| Cotswolds Design and Build Ltd | Construction | 2026 |
| Court House Estates | Real estate | 1935 |
| Craft Cider Limited | Manufacturing | 2019 |
| Crestpoint Wellness Ltd | Wholesale & retail | 2026 |
| CRS Training Services Limited | Transport & logistics | 2006 |
| CTT Lettings Ltd | Real estate | 2020 |
| Custard Designs Limited | Manufacturing | 2004 |
| Custom Moulded Polyurethane Limited | Manufacturing | 2003 |
| D Annetts Digital Consulting Ltd | Professional & scientific | 2023 |
| D M Powell Contracting Limited | Construction | 2011 |
| D&G Smart Repairs Limited | Wholesale & retail | 2024 |
| D. J. Voyce Building Contractors Limited | Construction | 2014 |
| D.& G. Bodyshops Limited | Extraterritorial | 2000 |
| D.F.L.Properties Limited | Real estate | 1966 |
| D.M.D.Solutions Limited | Construction | 2003 |
| D.R. Ivins Limited | Wholesale & retail | 2015 |
| DAJ Properties Limited | Real estate | 2000 |
| Damsell Associates Limited | Real estate | 2020 |
| DAN Garland Buildaholic Limited | Construction | 2021 |
| Dane Futures Limited | Administrative & support | 2011 |
| Dane Investments Limited | Administrative & support | 2005 |
| Dane Trading Group Limited | Administrative & support | 2011 |
| Danter's Car Parks Ltd | Other services | 2014 |
| Darren Rowles Building & Maintenance Ltd | Construction | 2010 |
| Data Exploration Limited | Information & communication | 2023 |
| DBC Solutions Ltd | Administrative & support | 2011 |
| Dean Cottages and Enterprises Ltd | Hospitality | 2014 |
| Dean Forest Railway Company Limited | Transport & logistics | 1981 |
| Dean Golding Limited | Construction | 2015 |
| Dean Turley Transport Ltd | Transport & logistics | 2022 |
| Deanbuild Properties Limited | Real estate | 1998 |
| Deangate Inns Ltd | Hospitality | 2022 |
| Deanscapes Limited | Administrative & support | 2024 |
| Dene Systems Limited | Information & communication | 2023 |
| Dental Engineering Solution Limited | Professional & scientific | 2013 |
| Dezign & Shine Ltd | Professional & scientific | 2022 |
| DGC Consulting and Management Limited | Administrative & support | 2010 |
| Dhruva Homes Ltd | Real estate | 2025 |
| Digital Galaxy Agency-Finesse Ltd | Information & communication | 2021 |
| Disruptive Labs Ltd | Wholesale & retail | 2025 |
| Divine Kidz Ltd | Education | 2018 |
| Djrsi Ltd | Information & communication | 2024 |
| DMB Locksmiths Ltd | Administrative & support | 2025 |
| Dobunni Finance Ltd | Financial services | 2020 |
| Doodle Delights Ltd | Wholesale & retail | 2023 |
| Double Barn Limited | Other services | 2022 |
| Douglas Bevan Consulting Ltd. | Professional & scientific | 2017 |
| Drakes Mead Management (NO 1) Limited | Households | 1991 |
| Dream Home Solutions (SW) Ltd | Construction | 2021 |
| Dreamsjd Consultancy Ltd | Administrative & support | 2026 |
| Drybrook Garage Limited | Wholesale & retail | 2003 |
| Dumco Limited | Wholesale & retail | 2010 |
| Duncastle Farm Estate Management Limited | Households | 2014 |
| Dwell Marketing Ltd | Professional & scientific | 2026 |
| Dynamic Process Simulation Limited | Mining & quarrying | 2000 |
| Dynamic Property Maintenance Limited | Construction | 2017 |
| Dynamic Unity Ltd | Health & social care | 2020 |
| Dynasty Media Limited | Information & communication | 2024 |
| E & a Barbershop Ltd | Other services | 2025 |
| E Goodwin & Sons (Funeral Directors) Limited | Professional & scientific | 1996 |
| E11EVN Limited | Hospitality | 2011 |
| East MON Developments Limited | Real estate | 1967 |
| East MON Holdings Limited | Real estate | 1971 |
| Eat@Theship Ltd | Hospitality | 2019 |
| Eclipse Body Management Ltd | Arts & recreation | 2010 |
| Ecoversityuk CIC | Agriculture & fishing | 2022 |
| Edentone Limited | Administrative & support | 1991 |
| Edesy Homecare Ltd | Administrative & support | 2013 |
| Edge Eletrical and Driver Hire Ltd. | Construction | 2014 |
| Edward Nicholas Fitness Ltd | Education | 2025 |
| EHP Holdings (2024) Limited | Professional & scientific | 2015 |
| Elite Defenders Limited | Wholesale & retail | 2021 |
| Emmray Limited | Real estate | 2026 |
| Empire Brands UK Sales Limited | Wholesale & retail | 2018 |
| Endgrain CNC Limited | Professional & scientific | 2025 |