| A & J Property Group Ltd | Real estate | 2024 |
| A & R Fishbar Ltd | Hospitality | 2019 |
| A Mcquillan Inspection Ltd | Professional & scientific | 2012 |
| A. & J. Shedden Limited | Agriculture & fishing | 1963 |
| A.C. Gutter Services Ltd | Construction | 2023 |
| A.M. Howie Ltd. | Transport & logistics | 1998 |
| A.Whitehead Properties Ltd | Real estate | 2023 |
| AA & KP Properties Ltd | Real estate | 2025 |
| Abbotsford Bar Limited | Wholesale & retail | 2025 |
| Accountancy Business Services Ltd | Professional & scientific | 2026 |
| Ace Home Developments Ltd | Construction | 2025 |
| Advance Plumbing and Heating Services Limited | Construction | 2002 |
| Advance Properties (Scotland) Ltd. | Real estate | 2006 |
| Aegis Self Storage Limited | Transport & logistics | 2026 |
| Aeternum Fife Limited | Arts & recreation | 2020 |
| Aetherion AI Limited | Information & communication | 2025 |
| Agh@Global Limited | Wholesale & retail | 2023 |
| Agrifit Ltd | Manufacturing | 2013 |
| Airvent Ltd | Construction | 2016 |
| Aksl Ltd | Transport & logistics | 2026 |
| Aliyan Baba Ltd | Transport & logistics | 2025 |
| ALL Seasons Outdoor Living Ltd | Manufacturing | 2024 |
| Alpcan Beauty Limited | Other services | 2023 |
| Alry Cutting Technology Ltd | Professional & scientific | 2017 |
| ALX & RBK Trans Ltd | Transport & logistics | 2018 |
| AMF Property's Scotland Ltd | Other services | 2015 |
| AMG GAS Services Ltd | Construction | 2017 |
| Anco Build Ltd | Construction | 2013 |
| Angel Nails and Spa HT Ltd | Other services | 2023 |
| Animal Central Chip and Scan Ltd | Agriculture & fishing | 2022 |
| Anoush Aesthetics Limited | Other services | 2022 |
| Aotrom Limited | Financial services | 2019 |
| Archie's Taxis Ltd | Transport & logistics | 2017 |
| Arronta Limited | Professional & scientific | 2012 |
| Auchterderran Store Ltd | Wholesale & retail | 2024 |
| Auchtertool Holdings Limited | Real estate | 2026 |
| Auchtertool Trading Company Ltd | Hospitality | 2018 |
| AWS Renewables (Scotland) Ltd | Professional & scientific | 2025 |
| B Stobbs Joinery and Building Contractor Limited | Construction | 2019 |
| B.C.M. Property Management Limited | Real estate | 2015 |
| B.Duffy Groundworks Ltd | Administrative & support | 2021 |
| B.I.I.Home Decor Ltd | Construction | 2026 |
| B.M. Plumbing & Heating Services Ltd | Construction | 2019 |
| Ballingry Store 1 Limited | Wholesale & retail | 2022 |
| Ballingry Store 2 Limited | Wholesale & retail | 2022 |
| Basebuilders (Fife) Limited | Construction | 2012 |
| Bay Engineering Limited | Wholesale & retail | 2021 |
| Bay Training Services Limited | Transport & logistics | 2021 |
| Bay Travel Coaches Limited | Transport & logistics | 2014 |
| Beccasbinaries Ltd | Information & communication | 2018 |
| Bell Recycling Fife Ltd | Water & waste | 2022 |
| Bell Trading (Fife) Ltd | Wholesale & retail | 2018 |
| Belton Roofing Ltd | Construction | 2021 |
| Benarty Carpets and Flooring Ltd | Wholesale & retail | 2019 |
| Benarty Chippy Ltd | Hospitality | 2025 |
| Benarty Community Forum | Other services | 2017 |
| Benarty Improvement Group | Other services | 2023 |
| Billy Mclean Building and Landscaping Ltd | Construction | 2022 |
| BIM Connect Limited | Professional & scientific | 2024 |
| Blairmill Limited | Real estate | 2023 |
| Blake Group Holdings Ltd | Financial services | 2024 |
| Blcp Developments Ltd | Construction | 2019 |
| BOB Millen Services Limited | Transport & logistics | 2018 |
| Body GLO Limited | Other services | 2004 |
| Bonnie Jeanie Limited | Financial services | 2024 |
| Bowhouse Equestrian Limited | Arts & recreation | 2010 |
| Bowhouse Group Limited | Construction | 2019 |
| Bright Heating Ltd | Construction | 2019 |
| Broadway Carpets (Scotland) Ltd | Wholesale & retail | 2015 |
| Bruce NDT Limited | Professional & scientific | 2014 |
| Bspoke Therapy Practice Ltd | Health & social care | 2026 |
| Buchanan Investments Limited | Financial services | 2024 |
| Burns Nights Limited | Construction | 2025 |
| Bynario Limited | Other services | 2019 |
| C-Fencing Ltd | Construction | 2025 |
| Cadet Concrete Systems Ltd | Construction | 2019 |
| Cadet Enterprises Ltd | Construction | 2016 |
| Cairngrey Ltd | Professional & scientific | 2025 |
| Cardenden 69 Limited | Extraterritorial | 2013 |
| Carlucci Ltd | Hospitality | 2021 |
| Carman Online Content Publishing Limited | Information & communication | 2011 |
| Cartmore Building Supply Company Limited | Construction | 1986 |
| Cartmore Transport Limited | Professional & scientific | 1988 |
| Cavelstone Aggregates Limited | Mining & quarrying | 2023 |
| Cavelstone Farm Limited | Agriculture & fishing | 1996 |
| CC Bloomers Sandwich Bar Ltd | Hospitality | 2020 |
| CD Form Solutions Ltd | Administrative & support | 2023 |
| CDA Autotech Ltd | Professional & scientific | 2023 |
| Cdrnapparel Ltd | Wholesale & retail | 2024 |
| Central Admin Services Limited | Administrative & support | 1989 |
| Chainlogic Ltd | Professional & scientific | 2025 |
| Charles Buchanan (Roughcasters) Limited | Construction | 2002 |
| Check Property Ltd | Real estate | 2024 |
| Clas-Sic Wafer FAB Limited | Manufacturing | 2017 |
| Clean N Go Ltd | Manufacturing | 2026 |
| Clemison Property Group Ltd | Real estate | 2025 |
| Cmac Pipework and Welding Services Ltd | Construction | 2026 |
| CMC Logistic Ltd | Transport & logistics | 2024 |
| CMJ Joinery Ltd | Administrative & support | 2017 |
| CMP Contracts Ltd | Manufacturing | 2016 |