| Flavour Cube Limited | Wholesale & retail | 2025 |
| Flexpod Living Ltd | Construction | 2025 |
| Flexx Creative Ltd | Professional & scientific | 2016 |
| Flint & Lime Limited | Construction | 2013 |
| Flipflop Design Limited | Information & communication | 2005 |
| Floating Islands Productions Limited | Information & communication | 2004 |
| Flora and Rose Ltd | Manufacturing | 2020 |
| Flourishing Leaders Ltd | Professional & scientific | 2020 |
| Flowceo Limited | Administrative & support | 2020 |
| FLY Sussex Limited | Extraterritorial | 2013 |
| Flybubble Ltd | Wholesale & retail | 2013 |
| Foley-Oates Ltd | Professional & scientific | 2014 |
| FOR Peat's Sake! Ltd | Arts & recreation | 2009 |
| FOR YOU Ltd | Administrative & support | 2015 |
| Forbes & Lomax Holdings Ltd | Manufacturing | 2023 |
| Forbes & Lomax Limited | Manufacturing | 1988 |
| Forbes Education Limited | Education | 2008 |
| Foster Landscapes Limited | Construction | 2016 |
| Foundry Events Ltd | Professional & scientific | 2017 |
| Four Hundred Communications Limited | Professional & scientific | 1998 |
| Fourpack Films Ltd | Information & communication | 2023 |
| FOX and Lion Limited | Wholesale & retail | 2022 |
| FOX Rockett Studio Ltd | Wholesale & retail | 2026 |
| Foxden Homes Limited | Real estate | 2015 |
| Francis Tate Marbleworks Limited | Construction | 2000 |
| Francisco Studio Limited | Arts & recreation | 2022 |
| Frazerhalsey Limited | Administrative & support | 2014 |
| FREEHOLD82 Limited | Households | 2020 |
| Freight Shop Ltd | Wholesale & retail | 2014 |
| Fresh Ambition Limited | Professional & scientific | 2008 |
| Fresh Design Studios Limited | Professional & scientific | 2012 |
| Fresh Tracks Engraving Ltd | Information & communication | 2018 |
| Freya Hughes Ltd | Professional & scientific | 2024 |
| Friday Street Holdings Limited | Construction | 2024 |
| Fringe Studio Ltd | Other services | 2023 |
| Frith Consultancy Services Limited | Professional & scientific | 2024 |
| Frontier Consultants Limited | Other services | 2015 |
| Frontier Oak Limited | Construction | 2023 |
| Frost Architectural Ltd | Professional & scientific | 2024 |
| Fulham Acupuncture and Nutrition Ltd | Health & social care | 2019 |
| Fuller's Equine Services Ltd | Agriculture & fishing | 2019 |
| Fumbalinas Limited | Wholesale & retail | 2023 |
| Fundraising Mosaics Ltd | Administrative & support | 2019 |
| Funky Boho Ltd | Wholesale & retail | 2012 |
| Furnace Wood Retreat Ltd | Hospitality | 2025 |
| Furness Publishing Ltd | Information & communication | 2013 |
| Future Fire Protection Ltd | Public administration | 2013 |
| Future Insight Analytics Ltd | Information & communication | 2023 |
| Future Proofing Solutions Limited | Construction | 2017 |
| Future Sunset Limited | Administrative & support | 2023 |
| Futurenow Digital Limited | Information & communication | 2016 |
| FX Glass Limited | Construction | 2023 |
| FX LAB Limited | Information & communication | 2014 |
| G & a Advisory Ltd | Professional & scientific | 2019 |
| G Cudlipp Ltd | Health & social care | 2012 |
| G D Milner Builders Ltd | Construction | 2025 |
| G M Taxis Lewes Ltd | Transport & logistics | 2012 |
| G.E. White & Sons Limited | Construction | 2008 |
| G.F. Sinclair Limited | Wholesale & retail | 1973 |
| Galaxy Technical Solutions Ltd | Construction | 2016 |
| Gallatrim Limited | Real estate | 1994 |
| Gallybird Ltd | Other services | 2017 |
| Gardeners Arms Lewes Limited | Hospitality | 2008 |
| Gardens & More Limited | Wholesale & retail | 2005 |
| Gaskell Consulting Ltd | Professional & scientific | 2023 |
| Gather Therapy CIC | Health & social care | 2019 |
| Gearey Ltd | Education | 2003 |
| Gemini Hairdressing Ltd | Other services | 2022 |
| Generation Distillers Ltd | Manufacturing | 2020 |
| Generator House Ltd | Financial services | 2020 |
| Genius Within C.I.C. | Professional & scientific | 2011 |
| GET Bikery CIC | Transport & logistics | 2024 |
| Getcrisp Limited | Professional & scientific | 2019 |
| GFJ Consulting Ltd | Information & communication | 2017 |
| Gfort Ventures Limited | Information & communication | 2021 |
| GFZ Holdings Limited | Extraterritorial | 2015 |
| GFZ Investments Limited | Construction | 2008 |
| Ghans Group Ltd | Professional & scientific | 2026 |
| Gifted Turtles Ltd | Wholesale & retail | 2017 |
| Gimungus Media Limited | Information & communication | 2018 |
| Glenvale Investments Ltd | Real estate | 2019 |
| Global EYE Care Limited | Professional & scientific | 2018 |
| Global Piping Solutions Ltd | Mining & quarrying | 2018 |
| Globe Intelligent Systems Limited | Manufacturing | 2025 |
| Gloucester Yard Management Limited | Households | 2001 |
| Glowplexion Ltd | Health & social care | 2011 |
| GLP Creations Ltd | Wholesale & retail | 2018 |
| Glynde and Beddingham Swimming Club CIC | Arts & recreation | 2023 |
| Glynde Forge Limited | Manufacturing | 2023 |
| Glynde, Beddingham & Firle Memorial Parade Limited | Other services | 2019 |
| Glyndebourne Enterprises Limited | Wholesale & retail | 2000 |
| Go Fitness Sussex Ltd | Arts & recreation | 2019 |
| Go Local Community Interest Company | Administrative & support | 2008 |
| Go Pest Limited | Construction | 2006 |
| Go Plastic Pallets Ltd | Administrative & support | 1998 |
| Goat and Monkey Productions Ltd | Arts & recreation | 2022 |
| Gold Fleck Limited | Administrative & support | 2019 |
| Goldfinch's DRY Cleaners Limited | Other services | 2010 |
| Goldleaf Commercial Ltd | Real estate | 2024 |
| Goldstone Laine Limited | Administrative & support | 2014 |