| Emma BTL Limited | Real estate | 2023 |
| Emma Projects Limited | Construction | 2022 |
| Emmet Press Ltd | Information & communication | 2025 |
| Enak Ltd | Hospitality | 2025 |
| Energy Technology and Control Limited | Manufacturing | 1988 |
| Engaging Interiors Limited | Professional & scientific | 2007 |
| Enganche 10 Limited | Professional & scientific | 2009 |
| Enkisoftware Limited | Information & communication | 2000 |
| Enlots Limited | Wholesale & retail | 2005 |
| Enteles Holdings Ltd | Financial services | 2023 |
| Enteles Search Limited | Administrative & support | 2019 |
| Enthum Coaching Ltd. | Professional & scientific | 2008 |
| Entire Electrical Services Limited | Construction | 2015 |
| Equestrian Wellbeing Collective CIC | Health & social care | 2026 |
| Equine Planet Ltd | Wholesale & retail | 2022 |
| Equisportif Ltd | Professional & scientific | 2022 |
| Erawan Limited | Hospitality | 2017 |
| Eriksson Building Services Limited | Construction | 2006 |
| Escher House Limited | Administrative & support | 2005 |
| Estate Planning Direct LLP | | 2023 |
| Esteamed DRY Cleaners (Lewes) Ltd | Other services | 2021 |
| Etch Limited | Arts & recreation | 2003 |
| Ether and Ember Ltd | Wholesale & retail | 2025 |
| Ethical-I Limited | Professional & scientific | 2020 |
| EU Eco Hub CIC | Health & social care | 2007 |
| Eurogolf Limited | Professional & scientific | 2001 |
| European Food and Farming Partnerships LLP | | 2010 |
| Evans Dodd LLP | | 2011 |
| Everett Medical Limited | Health & social care | 2019 |
| Everland, LLC | | 2019 |
| Everybodysmile Limited | Information & communication | 1998 |
| Everyday Projects Limited | Information & communication | 2007 |
| Evolutionary Love Ltd | Health & social care | 2023 |
| Evolved Acoustics Limited | Professional & scientific | 2022 |
| Evolving Events & Design Ltd | Professional & scientific | 2025 |
| Evprs Ltd | Professional & scientific | 2018 |
| EXO7 Ltd | Wholesale & retail | 2024 |
| Expat Network Limited | Information & communication | 2012 |
| Experience Wild LLP | | 2025 |
| Expertage Limited | Extraterritorial | 2004 |
| Expertview Limited | Administrative & support | 2006 |
| Explore Cabins Limited | Hospitality | 2025 |
| Explore Sussex Limited | Hospitality | 2025 |
| Expo Display Ltd | Manufacturing | 2025 |
| F N R Plant Hire Limited | Administrative & support | 2000 |
| F8 Story Ltd | Information & communication | 2024 |
| F9 Properties Ltd | Real estate | 2016 |
| Facebox Systems Limited | Extraterritorial | 2020 |
| Facilities and Building Management Limited | Administrative & support | 2010 |
| Fadacai Limited | Hospitality | 2026 |
| Fairfield House Partner Limited | Professional & scientific | 2024 |
| Faith Aviation Limited | Wholesale & retail | 2023 |
| Falcon Coffees Limited | Wholesale & retail | 2014 |
| Falcon Pension Trustees Limited | Financial services | 2024 |
| Falcon Trustees LLP | | 2025 |
| Faraday Resources Limited | Professional & scientific | 1999 |
| Farley Arts Trust | Arts & recreation | 2002 |
| Farleys House & Gallery Ltd | Arts & recreation | 1977 |
| Farmyard Rentals Limited | Administrative & support | 2025 |
| Farthings Garden Properties Ltd | Real estate | 2021 |
| Faster Horses Agency Ltd. | Professional & scientific | 2018 |
| FB Building and Property Services Limited | Construction | 2019 |
| FB Paul-Robert-Ies Limited | Real estate | 2023 |
| FCX Investments Limited | Financial services | 2022 |
| FE Anchors Ltd | Manufacturing | 2026 |
| Feature Plastering Ltd | Construction | 2026 |
| Feet Space Limited | Wholesale & retail | 2011 |
| Felix Homes Ltd | Real estate | 2022 |
| Ferrings Wood Limited | Agriculture & fishing | 2025 |
| FFS Connect Limited | Financial services | 2018 |
| Field and Frame Ltd | Professional & scientific | 2026 |
| Field Architecture Limited | Professional & scientific | 2004 |
| Fieldgroup Financial Services Ltd | Financial services | 2025 |
| Fieldtone Ltd | Manufacturing | 2024 |
| FIG and Olive Brighton Ltd | Hospitality | 2026 |
| Filtro Systems Ltd | Professional & scientific | 2025 |
| Financial Freedom Sooner Limited | Financial services | 2017 |
| Finch Coffee Roasters Limited | Manufacturing | 2023 |
| Find a Performer Ltd | Arts & recreation | 2003 |
| Finds App Ltd | Information & communication | 2026 |
| Finest Finishes Sussex Ltd | Construction | 2023 |
| Finnan Loveday Tailoring Limited | Manufacturing | 2022 |
| Finsdale Construction Company Limited | Construction | 1985 |
| Fionnlocha Wild Nature Sanctuary Ltd | Arts & recreation | 2025 |
| Fire in the Hole Productions Limited | Information & communication | 2019 |
| Firle Events Limited | Hospitality | 2010 |
| Firle Farms Limited | Agriculture & fishing | 1948 |
| Firle Health LLP | | 2024 |
| Firle Management Limited | Real estate | 1998 |
| Firle Place Limited | Administrative & support | 1997 |
| Firle Place Preservation Trust Limited | Arts & recreation | 1982 |
| Firle Trustees Limited | Financial services | 2025 |
| Firmwide Technology Limited | Information & communication | 2025 |
| Fisher Media Limited | Administrative & support | 2018 |
| Fishtail UK Ltd | Wholesale & retail | 2009 |
| Fisk Consultancy Lewes Limited | Professional & scientific | 2013 |
| Five Porches Consulting Limited | Education | 2017 |
| Five Porches Limited | Real estate | 2025 |
| Five Porches Properties Limited | Real estate | 2018 |
| Flagstaff Engineering Ltd | Construction | 2011 |