| Embryonex Communications Limited | Professional & scientific | 2001 |
| Emergency Plumbing Group 247 Limited | Construction | 2022 |
| Emicam Limited | Professional & scientific | 2022 |
| Emil Dale School of Performing Arts Limited | Education | 2011 |
| Emma Holcombe Ltd | Professional & scientific | 2018 |
| Emma Jeans Props Ltd | Arts & recreation | 2022 |
| EMP Logistic Ltd | Transport & logistics | 2019 |
| Emtl Properties Ltd | Real estate | 2024 |
| Encompass Property Solutions Limited | Construction | 2024 |
| Encore Search (Newburgh Street) Limited | Administrative & support | 2014 |
| Endangered Encounters Ltd | Professional & scientific | 2025 |
| Energi Coffee Limited | Manufacturing | 2021 |
| Enrez Interiors Ltd | Manufacturing | 2023 |
| Entertainment Go Ltd | Wholesale & retail | 2011 |
| Enyspace Ltd | Transport & logistics | 2019 |
| Equipment Rentals & Sales Limited | Administrative & support | 2012 |
| ERP Controls Consulting Ltd | Information & communication | 2018 |
| ESA Brands Ltd | Wholesale & retail | 2023 |
| Esmeralda Holding Ltd | Financial services | 2024 |
| Essential Safety Wear Limited | Wholesale & retail | 2006 |
| Essential Talents Recruitment Ltd | Education | 2023 |
| Essential Water Services (UK) Ltd | Construction | 2009 |
| Esthetically Pleasing Ltd | Other services | 2022 |
| Etiqe Ltd | Wholesale & retail | 2015 |
| Eunice Care and Recruitment Limited | Administrative & support | 2021 |
| Euroex Logistics Limited | Transport & logistics | 2007 |
| Eurolight Limited | Construction | 2016 |
| European Alternatives Limited | Education | 2007 |
| European Tyre Enterprise Limited | Professional & scientific | 2011 |
| EV Park Ltd | Construction | 2020 |
| EV Park Management Ltd | Construction | 2021 |
| EV Park RP Ltd | Construction | 2021 |
| Ev-S Hair & Beauty Limited | Other services | 2012 |
| Evenlina Limited | Manufacturing | 2021 |
| Event Life Limited | Administrative & support | 2022 |
| Eversince Limited | Wholesale & retail | 2006 |
| Eversley Estates Ltd | Construction | 2018 |
| Everyday Active Ltd | Arts & recreation | 2025 |
| Evestia Clinical Limited | Professional & scientific | 1996 |
| Evolution Health and Fitness Limited | Arts & recreation | 2006 |
| Evowc Ltd | Financial services | 2024 |
| EW Interiors Ltd | Wholesale & retail | 2024 |
| Exclusive Interiors by Roberto Occhipinti Ltd | Manufacturing | 2019 |
| Executive Valeting and Detailing Ltd | Wholesale & retail | 2025 |
| EXPD8 Solutions Ltd | Information & communication | 2001 |
| Express Direct Ltd | Transport & logistics | 2025 |
| Express Solutions Couriers Ltd Ltd | Transport & logistics | 2025 |
| Ezyvee Limited | Information & communication | 2018 |
| Ezza Rigging Ltd | Information & communication | 2021 |
| F X Properties Limited | Real estate | 2003 |
| Factor Three Ltd | Professional & scientific | 2017 |
| Factory Playhouse Productions Limited | Arts & recreation | 2019 |
| Fagan and Collins Properties Limited | Real estate | 2024 |
| Fairhaven Holdings Limited | Financial services | 2008 |
| Fairhill (Hemel Hempstead) Management Company Limited | Households | 1990 |
| Fairmeadows Limited | Construction | 2021 |
| Fairmile Health Limited | Administrative & support | 2015 |
| Fairmont Inspection and Certification Limited | Professional & scientific | 2021 |
| Fairmont International Training Services Limited | Education | 2022 |
| Falcon Intelligence Ltd | Administrative & support | 2025 |
| Falcons Lodge (Cardington) Managing Company Limited | Households | 2020 |
| Falkland Press Limited | Extraterritorial | 2009 |
| Falkland Press Property Limited | Extraterritorial | 2013 |
| Fansurge Limited | Information & communication | 2026 |
| Faramore Limited | Professional & scientific | 2024 |
| Faris MED Services Limited | Health & social care | 2011 |
| Farriers Mews Management Company Limited | Households | 2000 |
| Fatdrop Ltd | Information & communication | 2007 |
| Fatjack Lechworth Limited | Hospitality | 2019 |
| FB Chain Limited | Manufacturing | 1985 |
| FCM Markets Limited | Other services | 2010 |
| Fettle Bike Repair Ltd | Manufacturing | 2019 |
| FGH Controls Limited | Manufacturing | 1977 |
| FI Technology Holdings Ltd | Manufacturing | 2022 |
| FI. Technology Limited | Manufacturing | 1995 |
| Fidenda Group Limited | Professional & scientific | 2022 |
| Fidenda Limited | Information & communication | 2017 |
| Fidock Property & Design Ltd | Real estate | 2026 |
| Figures 4U Limited | Professional & scientific | 2003 |
| Financial Analytic Valley Ltd | Information & communication | 2013 |
| Findeventhire Ltd | Other services | 2018 |
| Finn House Limited | Real estate | 2024 |
| Finremit Global Solutions Ltd | Professional & scientific | 2020 |
| Finsbury Park Investments Limited | Real estate | 2024 |
| First Choice Scaffolding (GB) Ltd | Construction | 2019 |
| First Place Operations Limited | Administrative & support | 2025 |
| Fisan Holding UK Ltd | Financial services | 2024 |
| Fitness Aspirations Limited | Arts & recreation | 2009 |
| Five Rivers Office Limited | Real estate | 2025 |
| Five Star 62 Ltd | Other services | 2024 |
| Fivezerofive Limited | Information & communication | 2015 |
| Flaming Phoenix Limited | Information & communication | 2011 |
| Flat Four Consulting Ltd | Professional & scientific | 2018 |
| Flat Four Property Ltd | Real estate | 2019 |
| Flatpack Building Solutions Limited | Administrative & support | 2018 |
| Flawless Aesthetics UK Ltd | Extraterritorial | 2012 |
| Flawless Beauty by Sabina Ltd | Other services | 2023 |
| Flawless Slimming & Beauty Clinic Limited | Other services | 2021 |
| Fleet Source Limited | Professional & scientific | 2012 |
| Flex Shop Ltd | Wholesale & retail | 2025 |