| DPH Logistics and Supply Chain Ltd | Professional & scientific | 2023 |
| DR a B Weerasekara Ltd | Health & social care | 2014 |
| DR Keiron Lord Aesthetics Ltd | Health & social care | 2019 |
| DR S Weerasekara Ltd | Health & social care | 2014 |
| Dragon Convenience Store Ltd | Wholesale & retail | 2025 |
| Dragon Gym Leigh Ltd | Arts & recreation | 2025 |
| Dragonstone INK Ltd | Arts & recreation | 2026 |
| Dreams 2 Reality Competitions Ltd | Other services | 2020 |
| Drillco Europe Ltd | Mining & quarrying | 2016 |
| DRL Plumbing & Maintenance Limited | Construction | 2015 |
| Drmpainting Ltd | Extraterritorial | 2024 |
| DRP Consultation Ltd | Professional & scientific | 2021 |
| DS Luxe Limited | Wholesale & retail | 2026 |
| DSB Digital Ltd | Information & communication | 2025 |
| DSB Tech Consultancy Ltd | Information & communication | 2025 |
| Dspsuk Ltd | Construction | 2025 |
| DT Regional Limited | Administrative & support | 2025 |
| DTM Plumbing Ltd | Construction | 2026 |
| DU Passos Films Ltd | Information & communication | 2024 |
| Duet Food Plus Ltd | Wholesale & retail | 2025 |
| DUN RAF Limited | Transport & logistics | 2020 |
| Dunya Holding UK Ltd Ltd | Wholesale & retail | 2025 |
| Dustless Blasting UK Ltd | Administrative & support | 2015 |
| DW Steel Services Ltd | Construction | 2018 |
| DWS Health & Fitness Limited | Health & social care | 2022 |
| DWT Solutions (UK) Ltd | Water & waste | 2014 |
| Dynasty P Cleaners Ltd | Administrative & support | 2025 |
| East Bond Street Tyres Ltd | Transport & logistics | 2023 |
| East in the West Ltd | Hospitality | 2022 |
| East Lancs Self Storage Ltd | Real estate | 2017 |
| East Leigh AFC Limited | Arts & recreation | 2024 |
| Easy Save PVT Limited | Wholesale & retail | 2024 |
| Eaze Leisure Limited | Hospitality | 2020 |
| EBS Homecare Ltd | Construction | 2025 |
| EBS Homeserve Ltd | Construction | 2023 |
| Eccleston Engineering LLP | | 2013 |
| Eciiv Ltd | Wholesale & retail | 2019 |
| ECO2U Ltd | Construction | 2024 |
| Econova Development Limited | Construction | 2023 |
| Ecotox Laboratories Limited | Professional & scientific | 2022 |
| ED Trans Ltd | Transport & logistics | 2025 |
| Eddie Trans Logistic Ltd | Transport & logistics | 2017 |
| Edge Capital Group Recruitment Ltd | Administrative & support | 2025 |
| Edge Mind Limited | Professional & scientific | 2023 |
| Edusport Coaching Limited | Arts & recreation | 2015 |
| Edward & Father Transport Ltd | Transport & logistics | 2017 |
| Edwards Elevated Events Limited | Wholesale & retail | 2025 |
| Effective Hygiene Ltd | Administrative & support | 2020 |
| EFS Installations Ltd | Construction | 2017 |
| Egan Services Limited | Transport & logistics | 2025 |
| EGM Readycare Limited | Health & social care | 2022 |
| Ehibie Ltd | Administrative & support | 2024 |
| EHL Homes Limited | Real estate | 2025 |
| EKO Foods Global Limited | Wholesale & retail | 2025 |
| EKO Restaurant & Bar Ltd | Hospitality | 2024 |
| EL Loco Services Ltd | Health & social care | 2025 |
| EL Spaniardo Limited | Extraterritorial | 2023 |
| Elan Europe Limited | Manufacturing | 2023 |
| Elan Telecom Limited | Information & communication | 2011 |
| ELB Property Services Limited | Real estate | 2023 |
| Elektronks Ltd | Wholesale & retail | 2025 |
| Elevate Youth Homes Limited | Health & social care | 2025 |
| Elevate Youth Mindset Ltd | Education | 2025 |
| Elevra Corporation Ltd | Wholesale & retail | 2025 |
| Elite Access Solutions (EAS) Limited | Administrative & support | 2024 |
| Elite Bespoke Joinery Ltd | Construction | 2025 |
| Elite Detailershq Ltd | Construction | 2025 |
| Elite Fire Equipment Services Ltd | Administrative & support | 2024 |
| Elite Joinery (NW) Limited | Construction | 2024 |
| Ellickal Ltd | Wholesale & retail | 2023 |
| Elmae Consultancy Ltd | Education | 2024 |
| Elnur UK Limited | Wholesale & retail | 2000 |
| Elshaima B Mohammed Ltd | Wholesale & retail | 2024 |
| Elshaima Mohamed Limited | Professional & scientific | 2025 |
| Elvian Incorporation Limited | Wholesale & retail | 2026 |
| Elvis Eckardt Recruitment & Sales Solutions Limited | Professional & scientific | 2019 |
| Elysium Hair Replacement Clinic Limited | Other services | 2021 |
| Emerald Homecare Ltd | Public administration | 2026 |
| Empower Young Care Ltd | Health & social care | 2024 |
| Energise Contracting Ltd | Real estate | 2019 |
| Energy Utility Consultancy (UK) Limited | Administrative & support | 2008 |
| Engineered Floors & Interiors Ltd | Wholesale & retail | 2024 |
| Enlight Solutions Ltd | Professional & scientific | 2023 |
| Ensis Ltd | Professional & scientific | 2007 |
| Enterprise UK Precision Sheetmetal Ltd | Manufacturing | 2009 |
| Enviroparts Limited | Water & waste | 2016 |
| Envisiongrowth Ltd | Wholesale & retail | 2023 |
| EPD Environmental Limited | Professional & scientific | 2017 |
| Epworth Education Trust | Education | 2013 |
| ER Certification Ltd | Professional & scientific | 2009 |
| ERC Testing Ltd | Professional & scientific | 2015 |
| ERS Logistics Ltd | Administrative & support | 2022 |
| Esg-Un Ltd | Professional & scientific | 2024 |
| Eshop MN Ltd | Wholesale & retail | 2025 |
| Esparon's Property Development Limited | Construction | 2006 |
| Essien Ltd | Health & social care | 2025 |
| ETA Waste Management Limited | Water & waste | 2023 |
| Etofizzy Hair & Beauty Ltd | Other services | 2025 |
| European Market Leigh Ltd | Wholesale & retail | 2026 |
| European Mini Market 2 Ltd | Wholesale & retail | 2024 |