| Employal Ltd | Administrative & support | 2023 |
| Employee Zero Ltd | Information & communication | 2014 |
| Employment 4 ME Ltd | Administrative & support | 2023 |
| Employment Relations Services Limited | Administrative & support | 2001 |
| Emporia Accessories Ltd | Wholesale & retail | 2025 |
| Emporia Beds Limited | Wholesale & retail | 2012 |
| Emporio Fashion Ltd | Manufacturing | 2013 |
| Empower Therapy Ltd | Other services | 2025 |
| Empowered Homes Limited | Construction | 2020 |
| Empowering Hope Ltd | Hospitality | 2024 |
| Empowering Ladies Ltd | Other services | 2026 |
| Empress Pubs Limited | Hospitality | 2019 |
| EMR Consultancy Ltd | Information & communication | 2021 |
| Emre Direct Ltd | Manufacturing | 2024 |
| Emre Restaurant Ltd | Hospitality | 2020 |
| Emrite Ltd | Information & communication | 2017 |
| EMS Carz Limited | Wholesale & retail | 2016 |
| EMS Commerce Ltd | Wholesale & retail | 2025 |
| EMS Operations (UK) Ltd | Information & communication | 2016 |
| EMS Spares Online Limited | Wholesale & retail | 2023 |
| Ems-Fp&S Limited | Wholesale & retail | 1998 |
| Emsk Limited | Professional & scientific | 2018 |
| Emson Prop Limited | Real estate | 2016 |
| Emstair Homes Limited | Real estate | 1999 |
| Emsthetics Ltd | Other services | 2024 |
| Emtech Global Services Ltd | Transport & logistics | 2026 |
| EMY Studios Ltd | Manufacturing | 2020 |
| Emyfran Enterprise Ltd | Real estate | 2020 |
| Emzel Ltd | Information & communication | 2023 |
| EN6IN33R Ltd | Manufacturing | 2015 |
| ENA Consulting and Investment Ltd | Information & communication | 2006 |
| ENA Property Management Ltd | Construction | 2022 |
| Enable Inclusive Support Ltd | Health & social care | 2018 |
| Enable Manufacturing Ltd | Manufacturing | 2019 |
| Enable Social Care Services Ltd | Professional & scientific | 2024 |
| Enargia Consulting Limited | Information & communication | 2011 |
| Enato Logistics Limited | Transport & logistics | 2025 |
| Enay International Limited | | 1977 |
| Enbana Connect Ltd | Wholesale & retail | 2024 |
| Encap Solutions Ltd | Other services | 2014 |
| Encea Trans Ltd | Transport & logistics | 2016 |
| Enchanted Bell Tents Limited | Administrative & support | 2023 |
| Enchanted Dragon Limited | Hospitality | 2025 |
| Enchanted Vault Limited | Wholesale & retail | 2025 |
| Enchanting Healthcare Services Ltd | Health & social care | 2014 |
| Encourage Mental Health CIC | Education | 2022 |
| Encro Cars Ltd | Wholesale & retail | 2020 |
| END 2 END Support Solutions Limited | Professional & scientific | 2021 |
| END of Life Cars Limited | Water & waste | 2017 |
| END2END Consultant Limited | Information & communication | 2025 |
| Endale Projects Ltd | Hospitality | 2016 |
| Enderby Haulage Ltd | Transport & logistics | 2016 |
| Enderby Kebab House Ltd | Hospitality | 2024 |
| Enderby Scaffolding Solutions Ltd | Construction | 2025 |
| Endless Gaming Ltd | Manufacturing | 2022 |
| Endocure Ltd | Real estate | 2025 |
| Endosim Ltd | Wholesale & retail | 2025 |
| Endosoft Limited | Information & communication | 2006 |
| Enduvelop Limited | Professional & scientific | 2016 |
| Endzone Services Limited | Professional & scientific | 2020 |
| ENE Couriers Limited | Transport & logistics | 2019 |
| Enercomm Limited | Information & communication | 2017 |
| Energy 4 Businesses Limited | Administrative & support | 2016 |
| Energy Buzz Limited | Energy | 2015 |
| Energy Buzz Utilities Limited | Professional & scientific | 2020 |
| Energy Concerns Ltd | Construction | 2022 |
| Energy Efficiency Experts Limited | Other services | 2016 |
| Energy Efficiency Group Ltd | Construction | 2021 |
| Energy Key Trading (1993) Limited | | 1992 |
| Energy Performance Insights Ltd | Professional & scientific | 2024 |
| Energy Saving Assessors Limited | Professional & scientific | 2023 |
| Energy Space Systems Limited | Information & communication | 2023 |
| Energy Switch Ltd | Professional & scientific | 2024 |
| Energy Team Online Ltd | Professional & scientific | 2019 |
| Energy Unwrapped Limited | Professional & scientific | 2018 |
| Energy Wrap Ltd | Construction | 2025 |
| Energy ZOO Ltd | Extraterritorial | 2014 |
| Energysaversuk Ltd | Administrative & support | 2025 |
| Energywin UK Limited | Energy | 2014 |
| ENERGYWORX2 Limited | Construction | 2007 |
| Enerlytics AI Limited | Information & communication | 2025 |
| Enesse Ltd | Real estate | 2020 |
| ENF Properties Limited | Real estate | 2024 |
| ENFIELD10 Ltd | Real estate | 2026 |
| Engin Investments Ltd | Financial services | 2021 |
| Engine Dynamics Ltd | Professional & scientific | 2020 |
| Engine Experts Company Ltd | Wholesale & retail | 2026 |
| Engineered Power Installations Ltd | Construction | 2019 |
| Engineered Precision Ltd | Construction | 2020 |
| Engineering and Design Consultancy and Services UK Ltd | Professional & scientific | 2021 |
| Engineering Experience Ltd | Professional & scientific | 2025 |
| Engineering Servicess Ltd | Professional & scientific | 2023 |
| Engineroom Productions Limited | Administrative & support | 2000 |
| Enhance Holdings Ltd | Financial services | 2025 |
| Enhanced Joinery & Construction Ltd | Construction | 2019 |
| Enigma Industries Group Limited | Professional & scientific | 2023 |
| Enigma Information Solutions Limited | Information & communication | 2019 |
| Enigma Technical Solutions Limited | Professional & scientific | 2023 |
| Enigmex Machines Limited | Administrative & support | 2011 |
| Enipeter Limited | Public administration | 2020 |