| Dreamgenii Limited | Manufacturing | 2005 |
| Drinks Packaging Ltd | Wholesale & retail | 2015 |
| Dsqs Services Ltd | Professional & scientific | 2018 |
| DTP Painting Contractors Limited | Construction | 2025 |
| Duck Feathers Leek Ltd | Other services | 2026 |
| Dunmarb Limited | Financial services | 2021 |
| DY Cnuk Ltd | Wholesale & retail | 2023 |
| DZ Golf Ltd | Wholesale & retail | 2025 |
| Délice Ltd | Other services | 2018 |
| E D I Conveyors Limited | Professional & scientific | 2005 |
| E J S Property Limited | Real estate | 1999 |
| E.W. Lomas Limited | Transport & logistics | 1980 |
| Earley Mobility Solutions Ltd | Wholesale & retail | 2024 |
| Easyfix Emblems Holdings Limited | Financial services | 2017 |
| Easyfix Emblems Limited | Manufacturing | 1973 |
| Ecton Associates Ltd | Professional & scientific | 2023 |
| ED Brown Vehicle Repairs Limited | Wholesale & retail | 2025 |
| EDC Painters Ltd | Construction | 2019 |
| Edlu Holdings Limited | Financial services | 2020 |
| Edubolt Learning Ltd | Education | 2023 |
| Edward Whittaker Consultancy Ltd | Administrative & support | 2021 |
| EGG Well Farming Limited | Agriculture & fishing | 2021 |
| Elda Studios Limited | Arts & recreation | 2014 |
| Elif Bites Ltd | Hospitality | 2025 |
| Elms Electrical Contractors Limited | Construction | 2016 |
| Elnor Lane Artisan Produce Ltd | Manufacturing | 2020 |
| Elworth Plant Limited | Manufacturing | 2012 |
| Elytra Ltd | Professional & scientific | 2003 |
| Embrace Training & Recruitment Ltd | Education | 2024 |
| Emmi-Lou Dance Academy Ltd | Arts & recreation | 2014 |
| Emmie's Academy of Drama and Theatre Ltd | Arts & recreation | 2022 |
| Endeavour Catering Design and Project Management Ltd | Other services | 2020 |
| ENDURE52 Limited | Arts & recreation | 2025 |
| Eranja Limited | Financial services | 2021 |
| Eremos Ecotourism Ltd | Arts & recreation | 2025 |
| Esplanade Court Ltd | Real estate | 2017 |
| Essential Management Services Limited | Professional & scientific | 2007 |
| Esterchem (1) Limited | Manufacturing | 2022 |
| Esterchem (2) Limited | Manufacturing | 2026 |
| Esterchem (3) Limited | Manufacturing | 2026 |
| Esterchem Holdings Limited | Financial services | 2020 |
| Esterchem Limited | Manufacturing | 1998 |
| Eternis Fine Chemicals UK Limited | Manufacturing | 1960 |
| Etimos Ltd | Agriculture & fishing | 2020 |
| Euro Technic (UK) Limited | Administrative & support | 2005 |
| Evans & Wakerley Limited | Construction | 2021 |
| Eversafe Pest Solutions Ltd | Agriculture & fishing | 2024 |
| Excellis Europe Limited | Professional & scientific | 2014 |
| Exel Electrical Ltd | Construction | 2024 |
| Eyeriss Ltd | Information & communication | 2011 |
| F&L Home Interiors and Property Limited | Wholesale & retail | 2025 |
| Fairfield Property and Investments Ltd | Real estate | 2021 |
| Fast Food Leek Ltd | Hospitality | 2025 |
| Faulkner Powell Holdings Limited | Financial services | 2021 |
| Faulkner Powell Mortgages Limited | Financial services | 2021 |
| FBA Wizard Ltd | Information & communication | 2016 |
| Feeders R US Ltd | Agriculture & fishing | 2016 |
| Fell & Fire Sauna Co Ltd | Other services | 2026 |
| Filco Property Solutions Limited | Real estate | 2025 |
| Filtered Ltd | Administrative & support | 2024 |
| Finance Link Ltd | Financial services | 2017 |
| Finn TEC Commercials Limited | Wholesale & retail | 2014 |
| Firstplus Care Ltd | Administrative & support | 2019 |
| Fit Bodies Gym & Fitness Ltd | Arts & recreation | 2018 |
| Fixedfeeadvocacy Limited | Professional & scientific | 2012 |
| Flaming Leek (ST) Limited | Hospitality | 2024 |
| FLM Property Ltd | Real estate | 2026 |
| FLM Transport Ltd | Transport & logistics | 2026 |
| Flo's Diner Limited | Hospitality | 2023 |
| Flowsuite Limited | Information & communication | 2024 |
| Foker Grange Farm Limited | Agriculture & fishing | 2012 |
| Fortune Homes UK PVT Ltd | Construction | 2025 |
| Fortune UK PVT Ltd | Hospitality | 2023 |
| Four Fasteners Limited | Wholesale & retail | 2020 |
| Foxlowe Arts Centre Ltd | Arts & recreation | 2011 |
| Foxlowe Trading Company Limited | Hospitality | 2013 |
| Fozdan Ltd | Wholesale & retail | 2026 |
| Franchise Cleaning UK Ltd | Administrative & support | 2024 |
| Frandom Limited | Financial services | 2016 |
| Frodsham Vehicle Repair Ltd | Wholesale & retail | 2021 |
| G Gee's Cafe Ltd | Hospitality | 2026 |
| G S International Trucks Ltd | Wholesale & retail | 2009 |
| G&B Atvs Limited | Wholesale & retail | 2012 |
| G.E. Price Consultancy Ltd | Information & communication | 2019 |
| G.Stone Programming and Design Limited | Information & communication | 1985 |
| Gaddum & Gaddum Limited | Wholesale & retail | 2006 |
| Garden Plus Ltd | Administrative & support | 2022 |
| Gavel and Gown Limited | Information & communication | 1994 |
| Geekypixels Ltd | Information & communication | 2019 |
| GEN by Design Ltd | Construction | 2023 |
| Generalbond Limited | Real estate | 1998 |
| Genetix Fitness Limited | Arts & recreation | 2006 |
| George Frederick Cabinet Makers Ltd | Manufacturing | 2015 |
| George Gunia & SON Ltd | Wholesale & retail | 2012 |
| George's Tradition (Derby) Limited | Wholesale & retail | 2010 |
| GGP Marketing Limited | Professional & scientific | 2012 |
| Givans Naturals Ltd | Other services | 2024 |
| GJB Ltd | Professional & scientific | 2024 |
| Glademex Ltd | Construction | 1999 |
| Glencote Caravan Park Limited | Hospitality | 2025 |