| Rogerson Properties Limited | Wholesale & retail | 2001 |
| Rooscape Ltd | Arts & recreation | 2021 |
| Rotor Blade Technical Access Ltd | Construction | 2022 |
| Rourke Handbuilt Cycles Limited | Administrative & support | 2017 |
| Rudbrook Limited | Construction | 2019 |
| Rudyard Beauty Limited | Other services | 2014 |
| Rudyard Lake Railway Ltd | Transport & logistics | 2024 |
| Rushup Trading Ltd | Wholesale & retail | 2026 |
| Russell Street Dental Practice Limited | Health & social care | 2010 |
| Ryan Murrell Videography Limited | Information & communication | 2017 |
| Ryan Pantry Auto Services Limited | Wholesale & retail | 2017 |
| S & C Energy Limited | Energy | 2023 |
| Salmon Consultancy Limited | Professional & scientific | 2009 |
| Salt Leisure Ltd | Hospitality | 2026 |
| Saltire Court Management Company Limited | Households | 2020 |
| Sammons Architectural Limited | Professional & scientific | 2014 |
| Samuel Sigley & Sons Ltd | Other services | 2013 |
| Sanders Hill Ltd | Wholesale & retail | 2026 |
| Sanigin Limited | Manufacturing | 2015 |
| Sanjiao Holistics Limited | Education | 2015 |
| Sarga Takeaway Limited | Hospitality | 2026 |
| Savage Accountants Limited | Professional & scientific | 2014 |
| Saving Cross Global Care Limited | Administrative & support | 2022 |
| SB Medical Locums Limited | Health & social care | 2014 |
| Scabetti Limited | Manufacturing | 2012 |
| Scorpio Fast Food Ltd | Hospitality | 2020 |
| Scrumbles Ltd | Manufacturing | 2019 |
| SDB Plumbing & Heating Limited | Construction | 2019 |
| Searchwood Limited | Information & communication | 2009 |
| Sebanator Ltd | Transport & logistics | 2017 |
| Sell With Daryl Limited | Professional & scientific | 2025 |
| Sequre Limited | Real estate | 2018 |
| Shazlee Ltd | Hospitality | 2025 |
| Sherratt Finance Ltd | Financial services | 2024 |
| Shevolution Ltd | Administrative & support | 2023 |
| Silverdale Site Services Ltd | Mining & quarrying | 2015 |
| Simon Hoffman Limited | Administrative & support | 2014 |
| SIX Towns Design Ltd | Manufacturing | 2023 |
| Sleek Cars Ltd | Transport & logistics | 2022 |
| SM Rendering Contractors Ltd | Construction | 2019 |
| SM Sheldon Property Group Ltd | Real estate | 2025 |
| Smart GAP Property Solutions Ltd | Professional & scientific | 2020 |
| Smart PD & S Ltd | Construction | 2013 |
| Smartshoppersuk Limited | Wholesale & retail | 2022 |
| Smartshoppersukgb Limited | Wholesale & retail | 2024 |
| Smashit-It Ltd | Information & communication | 2026 |
| Snowdrop Property Limited | Real estate | 2018 |
| Snuggle Paws Ramble & Retreat Ltd | Other services | 2026 |
| SO Marketing (Agency) Ltd | Professional & scientific | 2019 |
| SO Marketing Limited | Information & communication | 2005 |
| Social Work 24 7 365 Ltd | Health & social care | 2018 |
| Soulandjazz.Com Limited | Information & communication | 2011 |
| South Cheshire DAB Ltd | Information & communication | 2021 |
| Space to Thrive Ltd. | Health & social care | 2015 |
| Spaceage Storage (UK) Ltd | Other services | 2008 |
| Sparrow Park Leek Ltd | Hospitality | 2020 |
| Spearons Ltd | Manufacturing | 2019 |
| Special Sauce International Ltd | Professional & scientific | 2024 |
| Special Sauce Ltd | Arts & recreation | 2014 |
| Spike Angel Ltd | Administrative & support | 2026 |
| Spikey Entertainment Ltd | Manufacturing | 2012 |
| Spout Brew House Limited | Hospitality | 2016 |
| Springbank Fencing Ltd | Construction | 2010 |
| Springwood Executive Ltd | Administrative & support | 2025 |
| Springwood Projects (Staffs) Ltd | Professional & scientific | 2023 |
| Sprinks Farm Shops Ltd | Wholesale & retail | 2022 |
| ST Edward Street Dental Practice Limited | Health & social care | 2020 |
| ST Edward's Church of England Academy Trust, Leek | Education | 2012 |
| Stafford Garage Doors Ltd | Construction | 2012 |
| Staffordshire Garden Designs Ltd | Construction | 2023 |
| Staffs & Moorlands Scaffolding Access Limited | Construction | 2024 |
| Stairwise Limited | Other services | 2020 |
| Stakeout Leek Limited | Hospitality | 2024 |
| Stele Direct Relief Care Ltd | Professional & scientific | 2023 |
| Stenbridge School of Ageing and Palliative Care Limited | Administrative & support | 2026 |
| Steph's Eco Cleaning Services Ltd | Administrative & support | 2025 |
| Stephen and Rebekka Clancy Ltd | Financial services | 2023 |
| Stoic Pilgrim Adventures C.I.C. | Other services | 2024 |
| Stoneoak Holdings Ltd | Construction | 2020 |
| Stonier Property Ltd | Real estate | 2021 |
| Stove Innovations Ltd | Manufacturing | 2021 |
| Stringers Recovery Limited | Transport & logistics | 2025 |
| Struthers & Co. Limited | Arts & recreation | 2024 |
| Struthers Watchmakers Limited | Manufacturing | 2014 |
| Stubblee Management Ltd | Households | 2019 |
| SUB-4 Limited | Health & social care | 2018 |
| SUB-4 Orthotics LAB Ltd | Manufacturing | 2026 |
| Sugden House Management Limited | Households | 2005 |
| Sugden Mews Freeholders Limited | Households | 2019 |
| Sunflowers Childcare Ltd | Health & social care | 2024 |
| Sunnyview Equine Ltd | Agriculture & fishing | 2015 |
| Sunspace Management Ltd | Professional & scientific | 2025 |
| Superficial Limited | Information & communication | 2018 |
| Supersport (Leisure Shirts) Limited | Manufacturing | 1986 |
| Systec Automation Ltd. | Manufacturing | 1995 |
| T M Lownds Limited | Transport & logistics | 2013 |
| T&C Birch Limited | Hospitality | 2020 |
| T. F. Travel Limited | Administrative & support | 1986 |
| Tailored Textiles Ltd | Other services | 2013 |
| Take Thyme Limited | Hospitality | 2016 |