| Science IS People Ltd | Professional & scientific | 2017 |
| Scotia Welding & Fabrication Limited | Manufacturing | 2001 |
| SFL Properties Ltd | Real estate | 2016 |
| Siamsoir Academy of Performance Irish Dance Ltd | Arts & recreation | 2010 |
| Simlauren Ltd. | Information & communication | 2013 |
| SLK HR Consulting Ltd | Other services | 2023 |
| Solar Future Scotland Limited | Energy | 2012 |
| South Queensferry Executive Travel Ltd. | Transport & logistics | 2014 |
| Stephen Runciman Limited | Construction | 2018 |
| Stockbridge Developments Ltd. | Real estate | 2005 |
| Symington Mackell Surveyors Limited | Real estate | 2003 |
| THE Black Bull Hotel (Borders) Limited | Hospitality | 2015 |
| THE Haggis Box Limited | Hospitality | 2020 |
| THE Haggis Cafe Limited | Hospitality | 2020 |
| THE High Street Nursery - Lauder Ltd | Health & social care | 2023 |
| THE Lauderdale Hotel Ltd | Hospitality | 2017 |
| THE Spotty Dog (Lauder) Limited | Wholesale & retail | 2010 |
| Thos a Shaw Holdings Limited | Real estate | 2025 |
| Tindalos Corporation Ltd | Manufacturing | 2025 |
| Town Hub Limited | Real estate | 2020 |
| Utilities Material Supplies Limited | Wholesale & retail | 2024 |
| Utilities Plant Hire Limited | Administrative & support | 2024 |
| Warriston Place Advisors Ltd. | Other services | 2016 |
| Weigh Greener CIC | Other services | 2019 |
| Wild Haggis Limited | Hospitality | 2018 |
| Wiselawmill Farm (Developments) Limited | Real estate | 2006 |
| Woodheads PIG Management Limited | Agriculture & fishing | 2022 |
| Wynd Group Limited | Wholesale & retail | 2018 |
| Yoga Mindful Movement Ltd | Extraterritorial | 2025 |