| Adam Purves & SON, Limited | Wholesale & retail | 1939 |
| AK Medical Imaging Ltd | Health & social care | 2026 |
| Allanbank Estates Limited | Real estate | 2020 |
| Arcvis Ltd | Administrative & support | 2023 |
| Arrowsmith, Bowman & Fletcher Limited | Information & communication | 2007 |
| AT Lifting Solutions Scotland Ltd. | Other services | 2017 |
| Balloch Nominees Limited | Extraterritorial | 2020 |
| Believe in Jesus Limited | Other services | 2019 |
| BEN Glanton Consulting Ltd | Information & communication | 2026 |
| Berwickshire Travel Limited | Administrative & support | 2024 |
| Bird Gardens Scotland CIC | Education | 2017 |
| Blue Shannon Consulting Ltd | Professional & scientific | 2015 |
| Bora Apps Limited | Professional & scientific | 2009 |
| Border Coatings (Scotland) Limited | Construction | 2004 |
| Borders Body & Mind Ltd. | Other services | 2004 |
| Bosco AI Ltd | Information & communication | 2024 |
| Bosco Dental Studio Ltd | Health & social care | 2025 |
| Bosco Group Holdings Ltd | Financial services | 2025 |
| Briardene Services Ltd | Professional & scientific | 2023 |
| Brightwire Electrical Solutions Ltd | Construction | 2026 |
| C & G Sharp Fabricators Limited | Manufacturing | 2011 |
| Caledonia Education Ltd | Education | 2020 |
| Cambara Properties Ltd | Real estate | 2010 |
| Campbells (Oxton) Limited | Transport & logistics | 2001 |
| Carfrae Farming Company | | 1989 |
| Carfrae Farming Company | | 1995 |
| Casey Divine Consulting Limited | Information & communication | 2015 |
| Castle Wynd Enterprises Ltd | Hospitality | 2025 |
| Castleview Holiday Lodges Limited | Hospitality | 2021 |
| CC Management Borders Ltd | Professional & scientific | 2023 |
| Chespond Limited | Professional & scientific | 2008 |
| Coast & Country Borders Limited | Hospitality | 2024 |
| Collegial Project Management Ltd. | Other services | 2024 |
| Corff Ltd | Real estate | 2020 |
| Corriegarth Ltd | Hospitality | 2016 |
| Dalply Limited | Construction | 2009 |
| Dana Campbell Consultants Limited | Professional & scientific | 2021 |
| DE Bhal Edwards Limited | Administrative & support | 2007 |
| Directly Personalised Limited | Manufacturing | 2016 |
| Dryburgh Consulting Limited | Information & communication | 2015 |
| E L Road Sweepers Ltd | Construction | 2012 |
| East Coast Cladding Ltd. | Construction | 2013 |
| Eden Medical Aesthetics Ltd | Health & social care | 2020 |
| Elliot & Associates Limited | Health & social care | 2015 |
| Engineering Evaluation Ltd | Professional & scientific | 2023 |
| Enterprise.Design.Works Ltd | Professional & scientific | 2026 |
| Fair Trade Electives Ltd | Administrative & support | 2024 |
| Faster Stronger Ltd | Arts & recreation | 2025 |
| Gerard Construction Ltd | Construction | 2011 |
| Gym Smart Ltd. | Administrative & support | 2008 |
| Horizon Residential Investments Ltd | Real estate | 2019 |
| ID Consultancy Solutions Limited | Professional & scientific | 2015 |
| Ifht Limited | Real estate | 2024 |
| Inntuitive Property Limited | Hospitality | 2018 |
| Itmg Ltd. | Information & communication | 2013 |
| J S Hardie (Electrical Contractors) Limited | Construction | 2010 |
| J. Connell Farms | | 2001 |
| J.J. Patterson | | 1988 |
| Jess Ltd | Professional & scientific | 2016 |
| King’S Road Dog Leisure Ltd | Other services | 2025 |
| Kitchen & Bathrooms Ltd. | Construction | 2016 |
| LA Change Solutions Ltd | Information & communication | 2025 |
| Lammermuir Gundogs Training Limited | Education | 2025 |
| Lauder Convenience Ltd | Wholesale & retail | 2020 |
| Lauderdale Coaches Ltd. | Transport & logistics | 2011 |
| Lauderdale Community Development Trust Ltd | Financial services | 2026 |
| Lauderdale Firewood Company Limited | Administrative & support | 2017 |
| Lauderdale Hire Limited | Administrative & support | 2018 |
| Lawson & Mcritchie Limited | Professional & scientific | 2022 |
| Leader Solutions Ltd. | Information & communication | 2010 |
| MARMARIS1 Ltd | Hospitality | 2017 |
| Mcbeth Photography Ltd | Professional & scientific | 2015 |
| Meishu Hairdressing Ltd | Other services | 2026 |
| Mole EX Ltd | Extraterritorial | 2024 |
| NG Contracting Ltd. | Construction | 2001 |
| Nmck Consulting Ltd | Professional & scientific | 2024 |
| NW Cladding Ltd. | Other services | 2020 |
| PAW Place Limited | Other services | 2026 |
| PDS Online Ltd. | Other services | 2020 |
| Pheonix Accountancy Ltd | Professional & scientific | 2010 |
| Phoenix Accountancy Plus Ltd | Administrative & support | 2014 |
| Premier Plus Holidays Ltd | Hospitality | 2025 |
| Purple Plum Lauder Ltd | Wholesale & retail | 2016 |
| Q Protect Ltd. | Other services | 2022 |
| Q Train Care Ltd | Education | 2019 |
| Q.Plus Ltd. | Professional & scientific | 2016 |
| Qtrain Ltd. | Education | 2011 |
| Quadcare Limited | Wholesale & retail | 2014 |
| R J Wilson Limited | Construction | 2014 |
| R Thomson Builders Ltd | Construction | 2022 |
| R Watson & SON (Plumbing & Heating) Limited | Construction | 2010 |
| Red Collar Consulting Limited | Information & communication | 2014 |
| Rockall Films Ltd | Information & communication | 2021 |
| Ronnie Anderson Ltd | Agriculture & fishing | 2020 |
| Roxburgh Financial Modelling Ltd | Professional & scientific | 2023 |
| Runciman Engineering Limited | Professional & scientific | 2018 |
| Safe Hands Scotland Ltd | Health & social care | 2024 |
| Safety Control Services Ltd | Mining & quarrying | 2024 |
| Saltire Roofing & Building Ltd. | Construction | 2007 |
| Saltire Solar Ltd | Energy | 2026 |