| Station Garage (Halton) Limited | Professional & scientific | 2012 |
| Stefan Robinson Ltd | Transport & logistics | 2025 |
| Stella SWS Limited | Financial services | 2019 |
| Steph Hoggarth Ltd | Professional & scientific | 2018 |
| Stephen Honeybone Engineering & Construction Services Limited | Construction | 2016 |
| Stephen M Wright Limited | Information & communication | 2006 |
| Stephenson Financial Planning Limited | Financial services | 2017 |
| Stephensons Roofing NW Ltd | Construction | 2022 |
| Sterling Capital Markets Ltd | Financial services | 2014 |
| Steven Dyer & Sons Limited | Real estate | 2020 |
| Stoddard Morecambe Limited | Households | 2012 |
| Stodday Land Limited | Construction | 2006 |
| Stone Garden Landscapes Ltd | Administrative & support | 2005 |
| Stoneacre Services Limited | Health & social care | 2024 |
| Stonewell TAP Ltd | Hospitality | 2017 |
| Storey Hall RTM Company Limited | Households | 2020 |
| Strad Media Limited | Information & communication | 1999 |
| Strata Commodities Limited | Wholesale & retail | 2026 |
| Strategic Energy Consulting Ltd | Professional & scientific | 2011 |
| Strawberry Fields Training C.I.C. | Education | 2008 |
| Stream Computer Services Ltd | Information & communication | 2021 |
| Street Earners Ltd | Wholesale & retail | 2025 |
| Stricklandgate Property Ltd | Real estate | 2016 |
| Stride Holdings Limited | Hospitality | 2023 |
| Stride Services Limited | Construction | 2021 |
| Stride Solutions Limited | Professional & scientific | 2003 |
| Student Housing Lancaster Limited | Real estate | 2012 |
| Studio 208 Productions Ltd | Arts & recreation | 2021 |
| Studio IVY Ltd | Other services | 2021 |
| Studio Tempest Limited | Information & communication | 2024 |
| Suan Pruksa Ltd | Wholesale & retail | 2023 |
| Successedition Limited | Wholesale & retail | 2026 |
| SUE Bridges Lettings Ltd | Real estate | 2016 |
| SUE Capstick Ltd | Health & social care | 2017 |
| SUE Shields Health & Beauty Studio Limited | Other services | 1997 |
| Sulli Barber Ltd | Other services | 2026 |
| Sultan of Lancaster Experience Limited | Hospitality | 2018 |
| Sultan's Cuisine Ltd | Hospitality | 2016 |
| Sumeria Properties Ltd | Real estate | 2019 |
| Summerlands Hall Ltd | Wholesale & retail | 2024 |
| Summit Energy Ltd | Construction | 2023 |
| Sun Street Studios Ltd | Hospitality | 2017 |
| Sunata Limited | Hospitality | 2024 |
| Sunny’S Culinary Creations Ltd | Hospitality | 2026 |
| Sunset Leisure Resorts Limited | Hospitality | 2022 |
| Sunset Park Limited | Hospitality | 2018 |
| Sunwood Developments Ltd | Real estate | 2005 |
| Supa Scaffold Construction Limited | Construction | 2024 |
| Superior Filtration Europe Limited | Manufacturing | 2006 |
| Supreme DRY Cleaners Limited | Other services | 2020 |
| Suspicious Bush Ltd | Information & communication | 2025 |
| Sutcliffe Accountants Ltd | Professional & scientific | 2013 |
| SVA Fast Food Ltd | Hospitality | 2025 |
| SVR Fast Food Ltd | Hospitality | 2021 |
| SW Transport 2021 Limited | Transport & logistics | 2021 |
| Sweat Studio Ltd | Health & social care | 2022 |
| Sweetweb Limited | Information & communication | 2014 |
| SWF Property Investments Limited | Real estate | 2025 |
| Swift Electrical Solutions Ltd | Construction | 2018 |
| Swift Fabrications (SW) Ltd | Manufacturing | 2020 |
| Swing Gate Properties Ltd | Real estate | 2023 |
| Swirl&Co Limited | Hospitality | 2026 |
| SWP Fitness Ltd | Other services | 2025 |
| Sydney's Enterprises Limited | Hospitality | 2019 |
| Sylvester Street Management Company Limited | Households | 2019 |
| Syma Therapies Limited | Health & social care | 2012 |
| SYS Integrator Ltd | Information & communication | 2012 |
| System Loco Ltd | Information & communication | 2016 |
| Systemsforecastinguk Ltd | Information & communication | 2018 |
| T & K PYE Family Ltd | Construction | 2013 |
| T & M Tree Management Ltd | Agriculture & fishing | 2019 |
| T & O Zhang Limited | Hospitality | 2017 |
| T Chapman Limited | Construction | 2002 |
| T Entwisle Plumbing and Heating Ltd | Construction | 2017 |
| T Jackson (Plumbing & Heating Services) Limited | Construction | 2008 |
| T Lettings Limited | Real estate | 2019 |
| T Simpson Electrical Ltd | Construction | 2020 |
| T Smith FS Ltd | Professional & scientific | 2020 |
| T Warburton Joinery Limited | Construction | 2023 |
| T Woodburn Ltd | Arts & recreation | 2026 |
| T&D Leisure Parks Ltd | Hospitality | 2026 |
| T&M Home Improvement Lancaster Ltd | Construction | 2025 |
| T.M. Noble Motor Engineers Limited | Wholesale & retail | 2002 |
| T.Rodgers & SON Ltd | Construction | 2022 |
| T3TTY Ltd | Wholesale & retail | 2002 |
| Tactical Security Options Limited | Administrative & support | 2016 |
| Tagapp Ltd | Financial services | 2021 |
| Tall Trees Lancaster Management Company Limited | Households | 2001 |
| Tangentworks UK Ltd | Information & communication | 2019 |
| Tanning Studio Preston Ltd | Other services | 2011 |
| Tarragon Commercial Solutions Limited | Professional & scientific | 2019 |
| Tasty WOK Chinese Ltd | Hospitality | 2024 |
| Tax & VAT Accountants Ltd | Professional & scientific | 2025 |
| Taylor Wootton Limited | Information & communication | 2015 |
| TB Forestry Ltd | Agriculture & fishing | 2018 |
| TB Services Ltd | Administrative & support | 2022 |
| TBL Services Ltd | Professional & scientific | 2016 |
| TCL Developments Limited | Construction | 2002 |
| TCL Project Consultants Limited | Professional & scientific | 2010 |
| Tcrv (Agent) Limited | Households | 2016 |