| Employment and Regeneration Partnership Limited | Administrative & support | 2000 |
| Emworth Property Limited | Households | 1976 |
| Encompass Information Services Ltd | Information & communication | 2006 |
| Engineered Capabilities Limited | Professional & scientific | 2006 |
| England Darts Organisation Limited | Arts & recreation | 2005 |
| Enlighhten Trading Limited | Wholesale & retail | 2026 |
| Enstone Newlyn Ltd | Wholesale & retail | 2025 |
| Enthios Training & Development Limited | Administrative & support | 2007 |
| Entwistle & Joynt Limited | Wholesale & retail | 1971 |
| Envirocare Midlands Limited | Administrative & support | 2001 |
| Environmental Renaissance Ltd | Other services | 1997 |
| Enzio Designs Limited | Wholesale & retail | 2007 |
| Enzo's Pizza Takeaway Ltd | Hospitality | 2011 |
| Epsilon Contracts Limited | Other services | 2002 |
| Eqeria Limited | Professional & scientific | 2008 |
| Equality and Diversity UK Limited | Professional & scientific | 2006 |
| Equatorial Consulting Limited | Information & communication | 2000 |
| Equinax Limited | Wholesale & retail | 2024 |
| Ericam Limited | Financial services | 2005 |
| Escort Taxis Limited | Information & communication | 2002 |
| ESS PEE Fabrications Limited | Manufacturing | 1964 |
| Essential GAS Services Ltd | Construction | 2016 |
| Established Legal Ltd | Administrative & support | 2006 |
| ETA Group Ltd. | Other services | 2006 |
| Euroflam Limited | Construction | 1992 |
| European Fragile Freight Limited | Professional & scientific | 1991 |
| Evapo Ltd | Wholesale & retail | 2014 |
| Evergreen Nebulisers Ltd | Health & social care | 2006 |
| Ewood Park Bakery Ltd. | Manufacturing | 2002 |
| Excell Hair & Beauty Limited | Other services | 2024 |
| Exchange Coffee Company Limited | Administrative & support | 1996 |
| Exel Properties Limited | Construction | 2003 |
| Express Currency Limited | Financial services | 2007 |
| Expressions of Lancaster Limited | Wholesale & retail | 1999 |
| Extralite Limited | Real estate | 1998 |
| Exyte UK Limited | Construction | 2024 |
| EYE Carers Limited | Health & social care | 1997 |
| EYE Designs Limited | Health & social care | 2001 |
| EYE2EYE Optical Birkenhead Ltd | Wholesale & retail | 2018 |
| EYE2EYE Optical Heswall Ltd | Wholesale & retail | 2018 |
| EYE2EYE Optical Upton Ltd | Wholesale & retail | 2018 |
| Eyekonic Images Limited | Information & communication | 2005 |
| Eysis Limited | Real estate | 2002 |
| F & F Motor Services Limited | Wholesale & retail | 1982 |
| F B Eminson & SON Limited | Agriculture & fishing | 2005 |
| F Bash Services Ltd | Real estate | 2020 |
| F I Construction Limited | Construction | 2018 |
| F I Focus Limited | Financial services | 2019 |
| F. & S. LEE & SON Limited | Wholesale & retail | 1972 |
| F.Crossley (Chemists) Limited | Wholesale & retail | 1955 |
| F.Oldfield Limited | Real estate | 1938 |
| Faber Plastering Contractors Limited | Construction | 2005 |
| Face 2 Face Estate Agents Ltd | Real estate | 2004 |
| Face 2 Face Mortgages Limited | Real estate | 2002 |
| Fairhaven Golf Club,Limited(The) | Arts & recreation | 1922 |
| Fairhurst Flats Limited | Households | 1972 |
| Fairhurst Limited | Financial services | 2001 |
| Fairhurst Ventures Limited | Real estate | 2007 |
| Fairplant Limited | | 1992 |
| Fairport Containers Limited | Manufacturing | 1993 |
| Fairport Holdings Limited | Professional & scientific | 1990 |
| Fairport Properties Limited | Real estate | 1982 |
| Fairport Storage Limited | Transport & logistics | 2006 |
| Fairway (Golf Shops) Limited | Wholesale & retail | 1997 |
| Faith & Hope Records Limited | Manufacturing | 1997 |
| Faith Based Delivery Ltd | Transport & logistics | 2008 |
| Fanshoop Ltd | Information & communication | 2025 |
| Farnel Milner Limited | Professional & scientific | 1999 |
| Fashionbreak Limited | Wholesale & retail | 1995 |
| Fassfern Forestry Limited | Agriculture & fishing | 2008 |
| Fast-Fix Workshop Limited | Wholesale & retail | 2002 |
| Fastline Superbikes Ltd | Wholesale & retail | 2008 |
| FAZ Enterprise Ltd | Health & social care | 2018 |
| Fenimore Limited | Wholesale & retail | 1996 |
| Fernhill Kirby Ltd | Wholesale & retail | 2025 |
| Fernside Construction Limited | Wholesale & retail | 1986 |
| FI Agricultural Limited | Construction | 2020 |
| Fieldfare Management Limited | Households | 1998 |
| Fielding Accountants Limited | Professional & scientific | 2002 |
| Fifthsgroup Ltd | Wholesale & retail | 2026 |
| File, Throw, DO Limited | Wholesale & retail | 2017 |
| Filhol Dental Manufacturing Limited | | 1982 |
| FINANCE4PHARMACY Ltd | Professional & scientific | 2008 |
| Financial & Professional Services Ltd | Professional & scientific | 2007 |
| Fine and Country CMB Limited | Professional & scientific | 2025 |
| Fine and Country Manchester Limited | Real estate | 2025 |
| Fine Stitch Fashion Ltd | Manufacturing | 2006 |
| Fire Equipment Services Limited | Manufacturing | 1996 |
| Fireacre Limited | Financial services | 1980 |
| Fireproof Fire Engineering Limited | Construction | 1982 |
| Firmcraft Limited | Professional & scientific | 2000 |
| First Choice Aluminium H & P Limited | Administrative & support | 2003 |
| First Steps Private Day Nursery Limited | Other services | 1998 |
| Five Star (Development) Homes Limited | Construction | 2002 |
| Five Star Blinds Ltd | Wholesale & retail | 2006 |
| Fleetwood Bowling Club Limited(The) | Arts & recreation | 1921 |
| Fleetwood Glass Limited | Wholesale & retail | 2002 |
| Fleetwood Laboratory Ltd | Administrative & support | 2002 |
| Fleetwood Service Centre Ltd | Wholesale & retail | 2002 |
| Floor-Form (NW) Ltd | Construction | 2003 |