| 1ST and 22ND Enterprises Limited | Hospitality | 2011 |
| 1ST Choice Horse Supplies (Scotland) Limited | Wholesale & retail | 2024 |
| 1ST Time Wright Limited | Administrative & support | 2023 |
| 2G Solutions (Scotland) Ltd | Manufacturing | 2021 |
| 3R Softwash Limited | Administrative & support | 2018 |
| 4 Player Games Ltd | Information & communication | 2024 |
| 4TH Tyres (UK) Ltd | Wholesale & retail | 2023 |
| 5 Mcjar Limited | Hospitality | 2021 |
| 5 Star Car Wash (Scotland) Ltd | Other services | 2014 |
| 5TH Avenue Cleans Ltd | Administrative & support | 2026 |
| 5W Property Ltd | Real estate | 2022 |
| 911 Solutions Limited | Administrative & support | 2014 |
| A & B Agri Limited | Agriculture & fishing | 2020 |
| A & J Law (Mobility Matters) Ltd | Other services | 2012 |
| A & J Law Group Ltd | Other services | 2012 |
| A C Banks Limited | Construction | 2003 |
| A D Plans Limited | Professional & scientific | 2004 |
| A Forster Properties Limited | Real estate | 2015 |
| A J Murdoch Limited | Wholesale & retail | 2008 |
| A L Campbell Haulage Ltd | Transport & logistics | 2026 |
| A T Whitelaw Consulting Ltd | Information & communication | 2015 |
| A&A Storry Limited | Construction | 2024 |
| A&B Carpentry Ltd | Construction | 2020 |
| A. Lennox Homes Limited | Construction | 2005 |
| A. R. Jackson Consulting Ltd | Professional & scientific | 2023 |
| A.D.Taxis Limited | Transport & logistics | 2011 |
| A2 Exterior Cleaning Ltd | Administrative & support | 2025 |
| A22 Consulting Ltd | Professional & scientific | 2025 |
| AB Landscaping Ltd. | Administrative & support | 2006 |
| Abbeygreen Ministries Trust | Other services | 2019 |
| Abbotsford Holdings Limited | Financial services | 2024 |
| Abernethy Homes Limited | Construction | 2019 |
| ABG Transport Services Ltd | Transport & logistics | 2017 |
| Ablesweep Limited | Administrative & support | 2026 |
| ABR Properties Wishaw Limited | Real estate | 2004 |
| AC Groundworks and Landscapes Ltd | Construction | 2023 |
| Ace Inspection Services Ltd | Professional & scientific | 2018 |
| Aceie Ltd | Other services | 2019 |
| ACL Decorators Limited | Construction | 2005 |
| Acorn Tree & Garden Services Ltd | Professional & scientific | 2024 |
| Acregate Limited | Construction | 2007 |
| ACS Ayrshire Cleaning Services Ltd | Administrative & support | 2023 |
| Active Engineering Solutions Ltd | Wholesale & retail | 2021 |
| Active Water Services Ltd | Water & waste | 2017 |
| ACW Contracts Ltd | Construction | 2021 |
| AD LOC Services Ltd | Transport & logistics | 2024 |
| Adair Engineering Ltd | Manufacturing | 2016 |
| Addie Facility Management Ltd | Construction | 2025 |
| Addison Reed Properties Limited | Real estate | 2021 |
| Adele Baird Ltd | Professional & scientific | 2025 |
| Adelp Financial Solutions Ltd | Financial services | 2013 |
| Advanced TAR Stone Limited | Construction | 2014 |
| Advizo Ltd | Information & communication | 2026 |
| AE Ferguson Civil Engineering Limited | Other services | 2022 |
| AEM (Scotland) Ltd | Wholesale & retail | 2025 |
| Aerial Vision Limited | Professional & scientific | 2017 |
| Aesteem Aesthetics Ltd | Health & social care | 2023 |
| Aesteem Aesthetics Scotland Limited | Health & social care | 2025 |
| African Skies Collective Ltd | Health & social care | 2026 |
| AG Auto Repairs Limited | Wholesale & retail | 2023 |
| AG Barr Holdings Limited | Wholesale & retail | 2017 |
| AG Barr Vehicle Repair Services Limited | Wholesale & retail | 2013 |
| Agalle Limited | Manufacturing | 2021 |
| Aggregate Production Consulting Limited | Professional & scientific | 2024 |
| Agrirural Consulting Limited | Professional & scientific | 2025 |
| AHR Project Management Services Ltd | Professional & scientific | 2012 |
| Ahtw Holdings Limited | Administrative & support | 2023 |
| Airmed GDI Limited | Manufacturing | 2018 |
| Airspace Unlimited Scotland Ltd | Transport & logistics | 2019 |
| AK OLE Solutions Limited | Professional & scientific | 2014 |
| Akach Homes Ltd | Real estate | 2025 |
| AKM Facilities Management Limited | Construction | 2026 |
| AKM Retail Solutions Limited | Construction | 2026 |
| Alan Elliot Butchers Ltd | Wholesale & retail | 2011 |
| Alan Pairman Logistics Ltd | Transport & logistics | 2018 |
| Albc (SCO) Limited | Other services | 2022 |
| Aldersyde Properties Limited | Real estate | 2024 |
| Aldersyde Transport Management & Audit Services Limited | Transport & logistics | 2026 |
| Alex Mcmeekin Ltd | Wholesale & retail | 2010 |
| Alex Young Bakers (Scotland) Ltd | Wholesale & retail | 2026 |
| Alexander Brooks & SON Limited | Wholesale & retail | 1962 |
| ALJ Industrial Supplies Ltd | Wholesale & retail | 2026 |
| Allan Alexander Mcleod Craft Butchers Ltd | Wholesale & retail | 2024 |
| Allander Psychiatry Limited | Health & social care | 2010 |
| Allure (Lanark) Limited | Other services | 2022 |
| Allwoods Construction Limited | Construction | 2014 |
| Almitas Training and Consultancy Limited | Education | 2015 |
| Alpine EHS Consultancy Ltd. | Professional & scientific | 2011 |
| Amas IT Ltd | Information & communication | 2013 |
| Amberfield Projects Ltd | Construction | 2025 |
| Amberstar Trading Limited | Hospitality | 2025 |
| Amcg Farming Limited | Agriculture & fishing | 2016 |
| Aminvest Holdings Ltd | Financial services | 2025 |
| Aminvest Property Ltd | Real estate | 2022 |
| Anas Barbers Ltd | Other services | 2025 |
| Anderson Holdings (Scotland) Limited | Wholesale & retail | 2025 |
| Anderson Philips Ltd | Administrative & support | 2025 |
| Anderson Tool Manufacturing Limited | Professional & scientific | 1994 |
| Andrew Maclean Design and Construction Management Limited | Construction | 2013 |
| Angel Nail & Spa Ltd | Other services | 2024 |