| ANN Penrice Limited | Agriculture & fishing | 2009 |
| Annbank Services Turfholm Garage Limited | Wholesale & retail | 2016 |
| Anserv Limited | Professional & scientific | 2008 |
| Anthony Gallo Acoustics Ltd | Wholesale & retail | 2016 |
| Antony Jolley Plumbing & Heating Engineer Ltd | Construction | 2015 |
| AR Haulage Limited | Transport & logistics | 2001 |
| Ardlevale Property Ltd | Real estate | 2026 |
| Ardmae Services Ltd | Wholesale & retail | 2024 |
| Ardmore Financial Planning Limited | Financial services | 2009 |
| Ardoch Design and Construction Ltd | Professional & scientific | 2016 |
| Arka Design Studio Ltd | Professional & scientific | 2007 |
| Arosm Ltd | Wholesale & retail | 2025 |
| Artisan Wishaw Limited | Hospitality | 2008 |
| ASA - Management Facilities Limited | Real estate | 2017 |
| Asac Care Ltd | Health & social care | 2016 |
| ASD Civils Limited | Construction | 2019 |
| ASD Group Scotland Ltd | Construction | 2025 |
| Astal Property Limited | Real estate | 2013 |
| Atlas Overseas Services L.P. | | 2014 |
| Auchinlea (Holdings) Limited | Wholesale & retail | 2017 |
| Audi Lovers Limited | Wholesale & retail | 2018 |
| Audio Hearing Services Ltd. | Professional & scientific | 2009 |
| Audrey's Coffees Limited | Hospitality | 2004 |
| Autotech Lanark Ltd | Wholesale & retail | 2019 |
| AV Transport Limited | Transport & logistics | 2008 |
| Avalanche DX Consulting Ltd | Professional & scientific | 2026 |
| Avalanche DX Ltd | Professional & scientific | 2025 |
| Aveerenz Limited | Construction | 2022 |
| Avenue Aesthetics & Beauty Ltd | Health & social care | 2024 |
| Avoca Flooring Limited | Wholesale & retail | 2020 |
| Avoca Properties and Lettings Ltd | Real estate | 2022 |
| AW Marinetech Ltd | Professional & scientific | 2025 |
| Aykhan Ltd | Health & social care | 2025 |
| B & B Contracts Ltd | Administrative & support | 2011 |
| Baby Scantastic Ltd | Health & social care | 2024 |
| Bailie Property Holdings Limited | Real estate | 2026 |
| Bains Fleet Services Ltd | Manufacturing | 2023 |
| Baleneq Limited | Professional & scientific | 2022 |
| Balgornie Enterprise and Employment Park Limited | Administrative & support | 2023 |
| Bamflatt BED and Breakfast Limited | Hospitality | 2014 |
| Bananas Bars (Lanark) Limited | Hospitality | 2025 |
| Bannatyne Holdings Ltd | Professional & scientific | 2023 |
| Barr Renewables Limited | Professional & scientific | 2011 |
| Baxter Bathrooms Limited | Construction | 2025 |
| BCD Services Limited | Professional & scientific | 2015 |
| Beeches Aesthetics Limited | Health & social care | 2024 |
| Belgrave Motor Engineers Limited | Wholesale & retail | 2015 |
| Belhaven Engineering Ltd. | Manufacturing | 2011 |
| Bella Footwear Limited | Wholesale & retail | 2025 |
| Beltay Limited | Real estate | 2025 |
| Belwood Development Limited | Construction | 2023 |
| Benson's Hair Limited | Other services | 2011 |
| Bentley Motors (1931) Limited | Wholesale & retail | 2006 |
| Big Time Operator Ltd | Information & communication | 2024 |
| Biggar Construction Limited | Construction | 2016 |
| Biggar Joiners Limited | Construction | 2022 |
| Biggar RFC Ltd | Hospitality | 2017 |
| Biggar Rugby Football Club | Arts & recreation | 2016 |
| Billscott Limited | Hospitality | 2022 |
| Birkhill MOT and Repair Centre Limited | Wholesale & retail | 2023 |
| Birkhill Storage & Commercial Units Ltd | Transport & logistics | 2025 |
| Black & Macdonald Ltd | Real estate | 2024 |
| Black Antler Woodworking Ltd | Manufacturing | 2024 |
| Black Tongue Developments Ltd. | Construction | 2005 |
| Blackout & Belladonna Ltd | Other services | 2025 |
| Blacks Heating Services Limited | Construction | 2022 |
| Blackwater Plant Training CIC | Education | 2026 |
| Blackwood Electrical Services Ltd | Wholesale & retail | 2026 |
| Blastpol UK Ltd | Construction | 2019 |
| Bloomgatebarbers Ltd | Other services | 2025 |
| Blue Audio Ltd. | Other services | 2016 |
| Bodhi Holistic Therapies Limited | Information & communication | 2014 |
| Bonningtons the Jeweller Limited | Wholesale & retail | 2014 |
| Border Biscuits Limited | Manufacturing | 1984 |
| Border Foods Limited | Extraterritorial | 1996 |
| Bowmuir Farm Holidays Ltd | Hospitality | 2022 |
| Braehead Property & Maintenance Limited | Construction | 2009 |
| Braidwood Developments Limited | Real estate | 2015 |
| Brand New Cake Limited | Manufacturing | 2010 |
| Braxfield Financial Services Ltd | Financial services | 2014 |
| BRC Plumbing & Heating Ltd | Construction | 2026 |
| Breakthrough Management Ltd | Professional & scientific | 2013 |
| Brennan and Co (Scotland) Ltd | Administrative & support | 2022 |
| Brian Alexander Limited | Agriculture & fishing | 2010 |
| Brian RAE Assessments Ltd | Professional & scientific | 2022 |
| Brian RAE Rail Services Limited | Manufacturing | 2018 |
| Brian Steele Joiners & Builders Limited | Construction | 2013 |
| Britbreed Limited | Professional & scientific | 2024 |
| British Texel Sheep Society Limited (the) | Other services | 1974 |
| Brocklinn Limited | Wholesale & retail | 2022 |
| Bronzilla Limited | Other services | 2025 |
| Brookside Innovations Ltd | Wholesale & retail | 2022 |
| Brookview Engineering Ltd | Professional & scientific | 2025 |
| Brotek Ltd | Wholesale & retail | 2013 |
| Brow Burn Limited | Professional & scientific | 2010 |
| Brow Burn Property Limited | Real estate | 2020 |
| Bruce Site Services Limited | Construction | 2023 |
| BS Autos MOT Centre Ltd | Wholesale & retail | 2023 |
| Budget Carglass Ltd | Wholesale & retail | 2011 |
| Buildingo Limited | Construction | 2023 |