| Heatmasters Fife Ltd | Construction | 2023 |
| Heaven Calling Ltd | Wholesale & retail | 2022 |
| Hellion Pictures Ltd | Information & communication | 2022 |
| Henry Sheach Kingdom Mowers Limited | Wholesale & retail | 2022 |
| Hestia Fire Safety Ltd | Public administration | 2025 |
| HEX Aesthetics Clinic Ltd | Health & social care | 2026 |
| HEX Media Ltd | Information & communication | 2013 |
| High Street Holdings Group Ltd | Information & communication | 2025 |
| High Street Kirkcaldy Limited | Wholesale & retail | 2025 |
| Highland Fashion & Workwear Ltd | Wholesale & retail | 2023 |
| Highwire Properties Ltd | Hospitality | 2015 |
| HIP Management Ltd | Real estate | 2018 |
| HIP Rentals Ltd | Real estate | 2016 |
| HMK Business Solutions Ltd | Hospitality | 2019 |
| HOLDING68 Ltd | Financial services | 2026 |
| Hollywood Roofing Contractors Ltd | Construction | 2015 |
| Holmes Cullen Developments Limited | Construction | 2025 |
| Home Improvement Digital Marketing Ltd | Professional & scientific | 2025 |
| Home of Hopcroft Limited | Wholesale & retail | 2020 |
| Homeish Limited | Real estate | 2020 |
| Hop-It Limited | Health & social care | 2025 |
| Horizon M&E Services Design Ltd | Professional & scientific | 2012 |
| House Clearance Collective Ltd | Water & waste | 2021 |
| House Windows & Doors Ltd | Construction | 2017 |
| Howe Inns Limited | Hospitality | 2005 |
| HS Elite Electricians - Trading AS Fife Electricians Ltd | Construction | 2025 |
| HSC Davis Limited | Arts & recreation | 2019 |
| HSS Restaurants Ltd | Hospitality | 2026 |
| HTF Fencing Limited | Manufacturing | 2019 |
| Hughes Construction Ltd | Construction | 2022 |
| Humble Investment Property's Ltd | Construction | 2025 |
| Hungry Wolf Café Scotland Limited | Other services | 2019 |
| I N M Building Services Limited | Construction | 2009 |
| IAN Johnston Funeral Directors Limited | Other services | 2019 |
| Ibits H Limited | Wholesale & retail | 2017 |
| Ibits R Limited | Information & communication | 2016 |
| IBN Direct Ltd | Wholesale & retail | 2023 |
| IBN Services Ltd | Other services | 2024 |
| IC Maintenance Services Limited | Construction | 2025 |
| Icms Property Limited | Real estate | 2025 |
| IDM Cost Management Ltd | Professional & scientific | 2019 |
| Idtc Limited | Education | 2006 |
| IFT Corporate Services Limited | Professional & scientific | 2016 |
| Igeenet Ltd | Wholesale & retail | 2013 |
| Igloo Habitat Limited | Administrative & support | 2013 |
| Iglu Studio Ltd | Professional & scientific | 2012 |
| Illuminous Solutions Ltd | Construction | 2022 |
| Imagin Products Ltd | Wholesale & retail | 2008 |
| IMH Hotels Ltd | Administrative & support | 2019 |
| IN House Care Group Ltd | Hospitality | 2020 |
| Inchdairnie AD Feedstock Ltd | Wholesale & retail | 2016 |
| Indulgence Fife Ltd | Hospitality | 2018 |
| Infinite SOL Ltd | Wholesale & retail | 2023 |
| Innes Johnston (Trustees) Limited | Professional & scientific | 2018 |
| Innes Johnston LLP | | 2015 |
| Innovate Foods Limited | Manufacturing | 1989 |
| Insure Smart Limited | Financial services | 2004 |
| Interlinked Alarms Ltd | Wholesale & retail | 2022 |
| International Lifting Services Ltd | Mining & quarrying | 2026 |
| Internet Creation Ltd. | Information & communication | 2002 |
| Intoto IT Services Ltd | Information & communication | 2020 |
| Inverleith Capital Limited | Extraterritorial | 2012 |
| Invobus Limited | Information & communication | 2017 |
| Ionisys Ltd | Professional & scientific | 2025 |
| Irek Ltd. | Extraterritorial | 2013 |
| Iris Art Studio Ltd | Arts & recreation | 2025 |
| IS IT Solutions Limited | Information & communication | 2024 |
| Isaac Gracie Limited | Other services | 2022 |
| Itasc Ltd | Information & communication | 2014 |
| Iteachcloud Ltd | Education | 2022 |
| Itechland (UK) Ltd | Information & communication | 2010 |
| ITS the Painter Ltd | Construction | 2025 |
| IVO & Tino S Limited | Transport & logistics | 2022 |
| J & P Fencing, Roofing and Landscaping Ltd | Construction | 2026 |
| J C Environmental Solutions Ltd | Water & waste | 2008 |
| J Cullen Ltd | Construction | 2023 |
| J Cullen Online Limited | Wholesale & retail | 2025 |
| J Gilmour & SON Limited | Construction | 2002 |
| J LI Glasgow Ltd | Hospitality | 2023 |
| J Mack Contracts Ltd | Construction | 2024 |
| J Wilson Joinery Ltd | Construction | 2022 |
| J.A.S. Safety Consultancy Ltd | Professional & scientific | 2008 |
| J.Allen Drainage Ltd | Water & waste | 2025 |
| J.M. Cochran Limited | Agriculture & fishing | 1956 |
| J.Michonski Ltd | Transport & logistics | 2025 |
| Jack Brownlee GAS, Heating & Plumbing Ltd | Construction | 2022 |
| Jack Hodson Rigging Ltd | Construction | 2023 |
| Jacks Snacks Ltd | Hospitality | 2026 |
| Jacksons Furnishings & Finds Ltd | Wholesale & retail | 2025 |
| JAD Motor London Ltd | Wholesale & retail | 2022 |
| Jade Garden Kirkcaldy Ltd | Hospitality | 2024 |
| Jagdis Properties Limited | Construction | 2009 |
| Jahb Construction Ltd | Construction | 2026 |
| Jalandhar Spice Ltd | | 2013 |
| JAM Installations (Fife) Ltd | Construction | 2024 |
| James C. Gillespie (Engineering) Limited | Manufacturing | 2002 |
| James C. Gillespie Limited | Professional & scientific | 2017 |
| James Parker Sculpture Limited | Manufacturing | 2014 |
| James S. White Ltd | Real estate | 2017 |
| Jamie Alexander Highlandwear Limited | Wholesale & retail | 2017 |