| Jamie Mcleary Golf Ltd | Wholesale & retail | 2021 |
| Janakraj Limited | Transport & logistics | 2024 |
| Jankowski Ltd | Extraterritorial | 2017 |
| Jarko Trans Ltd | Transport & logistics | 2023 |
| Javita Narang Ltd | Information & communication | 2021 |
| JB Roofing Scotland Ltd | Construction | 2023 |
| JCA Plumbers (Fife) Limited | Construction | 2016 |
| JCG ALL Trades Ltd | Construction | 2021 |
| JCG Bathrooms & Kitchens Ltd | Construction | 2022 |
| Jcgfabrication Limited | Manufacturing | 2018 |
| Jcsg Ltd | Real estate | 2017 |
| JD Removals and Delivery Ltd | Transport & logistics | 2024 |
| JDM Healthcare Ltd | Health & social care | 2020 |
| Jediah Limited | Professional & scientific | 2009 |
| Jemima Scotland Ltd | Professional & scientific | 2014 |
| Jezyk the Hedgehog Limited | Wholesale & retail | 2015 |
| JFK Joinery Ltd | Construction | 2018 |
| JHM Ltd | Administrative & support | 2024 |
| JJ Johnstone Limited | Manufacturing | 2011 |
| JJ Mcgill & SON Limited | Construction | 2024 |
| JJF Planning Ltd | Professional & scientific | 2025 |
| JKJ Homes Limited | Construction | 2023 |
| JKS Scotland Limited | Wholesale & retail | 2010 |
| JM Lifting and Rigging Services Ltd | Construction | 2018 |
| JM Motors (Fife) Limited | Wholesale & retail | 2016 |
| JMS Car Parts Ltd | Wholesale & retail | 2022 |
| JMS Stan Properties Ltd | Real estate | 2026 |
| Jmsn Fabrication Limited | Administrative & support | 2011 |
| JNL Property Group Ltd | Real estate | 2025 |
| Jo.Lly Cosmetics Ltd | Wholesale & retail | 2025 |
| John Anderson Fabrications Ltd | Manufacturing | 2018 |
| John Mathieson (Footfitters) Limited | Real estate | 1964 |
| John Reid & Sons (Crossgates) Limited | Extraterritorial | 1992 |
| Johnston and Johnston Fife Limited | Wholesale & retail | 2011 |
| Johnston Assets Limited | Administrative & support | 2013 |
| Johnston Crane Hire Limited | Extraterritorial | 2013 |
| Johnston Entertainments Ltd | Hospitality | 2018 |
| Johnston Rigging (Fife) Limited | Construction | 2003 |
| Johnston Rigging Holdings Limited | Financial services | 2013 |
| Joli Foods Limited | Wholesale & retail | 2024 |
| Jonathan Adam Racing Limited | Administrative & support | 2004 |
| Jonlin Engineering Limited | Manufacturing | 2009 |
| Jonnalagadda Ltd | Wholesale & retail | 2026 |
| Jordan Boats & CNC Limited | Manufacturing | 2013 |
| Jordan Denwette Roofing Ltd | Construction | 2025 |
| Jordan Valley Wholefoods Kirkcaldy Ltd | Hospitality | 2026 |
| JR Direct Roofing Ltd | Construction | 2022 |
| JRL Tech Ltd | Professional & scientific | 2016 |
| JRW Financial Management Ltd | Financial services | 2016 |
| JS Custom Threads Ltd | Manufacturing | 2025 |
| JS Inspections (Fife) Limited | Manufacturing | 2018 |
| JS Utilities Scotland Limited | Construction | 2026 |
| JSM Logistics Ltd | Transport & logistics | 2026 |
| Jsmd Properties Ltd | Real estate | 2020 |
| Justin Hughes Ltd | Financial services | 2023 |
| JWF Fabrications Limited | Manufacturing | 2017 |
| K & C Logistics Limited | Transport & logistics | 2009 |
| K & S Fabrication (Fife) Ltd | Manufacturing | 2019 |
| K a Fabrications Ltd | Manufacturing | 2025 |
| K Dental Laboratory Limited | Health & social care | 2007 |
| K M Construction (Fife) Limited | Construction | 2013 |
| K Scaff Kirkcaldy Ltd | Construction | 2019 |
| K Wallace Joinery Ltd | Construction | 2012 |
| K&S Exports Ltd | Wholesale & retail | 2017 |
| K. Schiavone Architecture Ltd | Professional & scientific | 2025 |
| K.Md Discos Ltd | Arts & recreation | 2024 |
| KAI Inspection & Testing Ltd | Professional & scientific | 2025 |
| KAI Resourcing Group Limited | Administrative & support | 2025 |
| Kalopsia C.I.C. | Manufacturing | 2013 |
| Kamp Coffee Ltd | Wholesale & retail | 2025 |
| Kapture Limited | Professional & scientific | 2024 |
| Kass Logistics Limited | Transport & logistics | 2025 |
| Kaur Properties(Fife) Limited | Real estate | 2021 |
| KAY Joinery Ltd | Construction | 2012 |
| KB Vidak Limited | Financial services | 2026 |
| Kboka Security Services Ltd | Administrative & support | 2024 |
| KBS Brox Limited | Hospitality | 2023 |
| KC Properties Fife Ltd | Real estate | 2026 |
| KCJ Engineering Services Ltd | Professional & scientific | 2023 |
| Kdyautocare Ltd | Wholesale & retail | 2026 |
| KE Automatics Limited | Arts & recreation | 1988 |
| Keane Trends Limited | Wholesale & retail | 2019 |
| Keep in Touch Electronics Ltd | Wholesale & retail | 2026 |
| Kendlebell (Fife) Ltd | Professional & scientific | 2008 |
| Kengor Group Ltd | Construction | 2025 |
| Kevin Smith Slaters Limited | Construction | 2004 |
| KGA Electrical Services Limited | Construction | 2012 |
| KGA Services Limited | Construction | 2022 |
| Khalsa Spice Ltd | Hospitality | 2022 |
| Khans Scot Ltd | Hospitality | 2025 |
| Kilgraston Sports Centre Ltd | Arts & recreation | 2026 |
| Kilted Generation Limited | Arts & recreation | 2010 |
| Kingdom Abuse Survivors Project | Health & social care | 2016 |
| Kingdom Autobody Repairs Ltd | Wholesale & retail | 2019 |
| Kingdom Bathrooms & Tiles Ltd. | Wholesale & retail | 2014 |
| Kingdom Clear Window Cleaning Limited | Administrative & support | 2012 |
| Kingdom Electricians Fife Ltd | Construction | 2018 |
| Kingdom Financial Services Ltd | Professional & scientific | 2013 |
| Kingdom HCS Limited | Administrative & support | 2016 |
| Kingdom Holiday Lets Ltd | Hospitality | 2019 |