| Fife Creamery Limited | Wholesale & retail | 1979 |
| Fife Cultural Services Trading Limited | Arts & recreation | 2012 |
| Fife Cultural Trust | Arts & recreation | 2012 |
| Fife ECU Re-Mapping Ltd | Wholesale & retail | 2020 |
| Fife Electrical Services Ltd | Construction | 2022 |
| Fife Facility Services Ltd | Construction | 2021 |
| Fife Federation of Tenants & Residents Associations (Ffotra) Limited | Other services | 2015 |
| Fife Flyers 2025 Ltd | Arts & recreation | 2025 |
| Fife Gardening Services Ltd | Administrative & support | 2013 |
| Fife Golf Limited | Arts & recreation | 2025 |
| Fife Group Limited | Transport & logistics | 1972 |
| Fife Home Improvement Ltd | Construction | 2022 |
| Fife Intensive Rehabilitation and Substance USE Team (First) Limited | Health & social care | 2006 |
| Fife Landscapes & Artificial Grass Limited | Administrative & support | 2020 |
| Fife Laser Limited | Manufacturing | 2024 |
| Fife Lifting Solutions Ltd | Construction | 2025 |
| Fife Mobile Accident Repair Service Limited | Wholesale & retail | 2025 |
| Fife Movers Ltd | Transport & logistics | 2024 |
| Fife Print Centre Ltd | Extraterritorial | 2017 |
| Fife Property Developments Limited | Real estate | 2017 |
| Fife Property Investments Limited | Real estate | 2015 |
| Fife Rape and Sexual Assault Centre | Health & social care | 2007 |
| Fife Real Estate Limited | Construction | 2018 |
| Fife Renovations Ltd | Construction | 2017 |
| Fife Scot Properties Limited | Real estate | 2026 |
| Fife Seat Limited | Wholesale & retail | 2007 |
| Fife Shopfitting Services Limited | Manufacturing | 1988 |
| Fife Signs Limited | Extraterritorial | 1989 |
| Fife Smart Repair Specialist Limited | Manufacturing | 2018 |
| Fife Sprayers Ltd | Construction | 2024 |
| Fife Storage Centre Ltd | Transport & logistics | 2022 |
| Fife Stoves Ltd | Construction | 2025 |
| Fife Training Services Limited | Professional & scientific | 1963 |
| Fife Vehicle Hire Ltd | Administrative & support | 2026 |
| Fife Welding Supplies Ltd | Administrative & support | 2018 |
| Fifediy Ltd | Other services | 2017 |
| Fifeshire Logistics (Scotland) Limited | Transport & logistics | 2019 |
| Fifty One Fifty One 100 Ltd | Hospitality | 2018 |
| Filmed With Love Limited | Professional & scientific | 2024 |
| Finesse Windows & Interiors Ltd. | Construction | 1999 |
| First Class Barber Shop Ltd | Other services | 2023 |
| First Horizon Scotland Ltd | Wholesale & retail | 2020 |
| First Plumb Solutions Limited | Construction | 2024 |
| Fluid Pumping Services Ltd | Manufacturing | 2026 |
| FN Enterprise (Scotland) Limited | Wholesale & retail | 2024 |
| Fone Click Limited | Wholesale & retail | 2020 |
| Forber Survey Ltd | Professional & scientific | 2021 |
| Forbo Floors UK Limited | Manufacturing | 1964 |
| Fords Daly Legal Limited | Professional & scientific | 2013 |
| Forth Autos Ltd | Wholesale & retail | 2025 |
| Forth Avenue Grocers Limited | Wholesale & retail | 2025 |
| Forth Developments Ltd | Real estate | 2016 |
| Forth GAS Limited | Construction | 2018 |
| Forth Office Supplies Limited | Wholesale & retail | 2012 |
| Forth Plumbing and Heating Limited | Construction | 2018 |
| Forth Property Solutions Ltd | Real estate | 2025 |
| Foss Entertainments and Building Company Limited | Construction | 1980 |
| FOX Media Management Ltd | Professional & scientific | 2025 |
| Frankie Williams Consulting Ltd | Professional & scientific | 2022 |
| Freedom Achieved Ltd | Hospitality | 2022 |
| Friendlieschinesefood Limited | Hospitality | 2025 |
| Frontline Fife Homelessness Services | Health & social care | 1997 |
| Frostkrone UK Limited | Financial services | 2020 |
| Future Pick Ltd | Wholesale & retail | 2026 |
| Future YOU Recruitment Ltd | Administrative & support | 2017 |
| Future-Proof Fashion Ltd | Professional & scientific | 2023 |
| Futurestepper Ltd | Information & communication | 2025 |
| Fyfe Group Limited | Professional & scientific | 2023 |
| FZ Systems Ltd | Wholesale & retail | 2026 |
| G Kelly Ltd | Manufacturing | 2016 |
| G Linton Taekwon-Do LLP | | 2017 |
| G&Js Investments Limited | Real estate | 2023 |
| G.B. Bricklaying Contracts Ltd. | Construction | 2007 |
| G1 Reeds Limited | Manufacturing | 2014 |
| G20 Project Services Ltd | Construction | 2022 |
| GA Investco Ltd | Financial services | 2023 |
| Gallatown Bike Hub Ltd | Wholesale & retail | 2016 |
| Galy Shine Limited | Wholesale & retail | 2021 |
| Garage Glow Limited | Construction | 2025 |
| Garden Rooms by Concept Limited | Construction | 2020 |
| Garden Wright Services Ltd | Administrative & support | 2023 |
| Gartshore Properties Ltd | Administrative & support | 2025 |
| GAS Solutions (Scotland) Limited | Wholesale & retail | 1999 |
| GB Quality Consulting Services Limited | Professional & scientific | 2017 |
| Gdeamer Services Ltd | Construction | 2022 |
| GDI Lettings Ltd | Hospitality | 2021 |
| Gemini Group Holdings Ltd | Transport & logistics | 2022 |
| Gentle Hands Healthcare Limited | Health & social care | 2022 |
| German Kitchen Studio Limited | Wholesale & retail | 2012 |
| Gerry Farrell INK Ltd | Professional & scientific | 2015 |
| Gfjk Ltd | Manufacturing | 2012 |
| GG Central Ltd | Professional & scientific | 2026 |
| GGS Takeaway Limited | Hospitality | 2025 |
| GH Pipe Fabrication Ltd | Manufacturing | 2017 |
| Gibson & Gilham Hair and Beauty Ltd | Other services | 2021 |
| GIS Platforms Limited | Information & communication | 2024 |
| Glen Fife Ltd | Real estate | 2025 |
| Glenachulish Developments Ltd | Real estate | 2021 |
| Glenalmond Enterprises Ltd | Administrative & support | 2011 |
| Glencoe Cottage Ltd | Real estate | 2019 |