| Dysart Premier Limited | Wholesale & retail | 2012 |
| Dysart Property Limited | Real estate | 2017 |
| Dyslexia Assessments Scotland Limited | Education | 2016 |
| Dziulapl Ltd | Transport & logistics | 2025 |
| E D Hotel Ltd | Hospitality | 2026 |
| E1 Property Management Ltd | Real estate | 2026 |
| EA Properties Group Ltd | Real estate | 2026 |
| East Coast Property Specialists Ltd | Real estate | 2023 |
| East Coast Rope Access Solutions Ltd | Mining & quarrying | 2026 |
| East Coast Window Cleaners Ltd | Administrative & support | 2019 |
| East Fife Handyman Ltd | Construction | 2026 |
| Easy Garage Fife Ltd | Wholesale & retail | 2017 |
| EAT & Go Phoenix Food Company Limited | Manufacturing | 2007 |
| Ebike Factory Limited | Manufacturing | 2024 |
| Eco Alba Limited | Administrative & support | 2013 |
| Eco Scot Ltd | Extraterritorial | 2021 |
| Eco Sips Homes Limited | Wholesale & retail | 2017 |
| Ecobean Artisan Coffee Limited | Wholesale & retail | 2026 |
| Ecobean Limited | Hospitality | 2020 |
| Ecosse Aero NDT Limited | Manufacturing | 2022 |
| Ecosse Energy Solutions Limited | Real estate | 2016 |
| Ecosse Tourers Ltd | Administrative & support | 2014 |
| ED Event Ltd | Arts & recreation | 2024 |
| Edenbrook (Fife) Limited | Manufacturing | 2012 |
| Edifice Pharma Ltd | Wholesale & retail | 2025 |
| Edifice Properties & Investments Ltd | Real estate | 2022 |
| Edin Clean Ltd | Administrative & support | 2022 |
| Edinb Services Ltd | Transport & logistics | 2026 |
| Edinburgh Blacksmiths Limited | Manufacturing | 2026 |
| Edinburgh Chihuahua Cafe Ltd. | Hospitality | 2017 |
| Eevitronics Ltd | Manufacturing | 2026 |
| Effero Limited | Information & communication | 2025 |
| EFR Studios Ltd | Information & communication | 2020 |
| EK Aesthetics Ltd | Other services | 2026 |
| Elaine Dall Hypnotherapy & Well-Being Ltd | Health & social care | 2018 |
| Elder Coaching and Development Ltd | Education | 2024 |
| Elder Offshore Services Ltd | Mining & quarrying | 2024 |
| Elder Preston Properties Limited | Real estate | 2025 |
| Electrifife Ltd | Construction | 2025 |
| Electron Garage Ltd | Wholesale & retail | 2019 |
| Electrotechnical Consultancy Services Limited | Mining & quarrying | 2015 |
| Elevation Property Group Ltd | Construction | 2025 |
| Elfet Ltd | Agriculture & fishing | 2018 |
| Elite Automotive (Scotland) Ltd | Wholesale & retail | 2014 |
| Elite Falconry Limited | Administrative & support | 2024 |
| Ellie Services Ltd | Administrative & support | 2017 |
| Ellis Taxis Limited | Wholesale & retail | 2006 |
| Embed Software Limited | Information & communication | 2022 |
| Ember and INK Prints Limited | Manufacturing | 2025 |
| Emerge Tech Ltd | Wholesale & retail | 2022 |
| Emilia Logistics Limited | Transport & logistics | 2020 |
| Empire Loyal Limited | Professional & scientific | 2016 |
| Empowerella Ltd | Manufacturing | 2025 |
| Empyrean Asset Holdings Ltd | Construction | 2023 |
| EMS Delivery Service Ltd | Transport & logistics | 2026 |
| Ence Catering Ltd | Hospitality | 2022 |
| Enviro-Wash Solutions Ltd | Construction | 2020 |
| Erlido Limited | Wholesale & retail | 2026 |
| Esgray Property Co Limited | Real estate | 2026 |
| Eskohl Limited | Wholesale & retail | 2023 |
| Essamo Property Limited | Real estate | 2025 |
| Esteem Lettings Limited | Real estate | 2013 |
| Euan Maxwell Development Limited | Information & communication | 2019 |
| Euchrid Consulting Ltd | Information & communication | 2022 |
| EVA Harte Ltd | Real estate | 2018 |
| Evan Restaurant Ltd | Hospitality | 2024 |
| Everlastings Holdings Limited | Financial services | 2021 |
| Everlettings Ltd | Real estate | 2021 |
| Everlight Power UK Limited | Professional & scientific | 2024 |
| Evoke Estates Ltd | Real estate | 2025 |
| Ewan Mclean Limited | Construction | 2013 |
| EWP Connectors Limited | Wholesale & retail | 2026 |
| Excel My Event UK Ltd | Administrative & support | 2017 |
| Excelsior Care Limited | Real estate | 2016 |
| F & M Developments (Shetland) Ltd. | Other services | 2006 |
| F and F Coffey Ltd | Health & social care | 2016 |
| F Scott Roofing & Roughcasting Fife Ltd | Construction | 2021 |
| F Scott Roofing & Roughcasting Ltd | Construction | 2020 |
| Fabric Fusion ZB Ltd | Wholesale & retail | 2024 |
| Fair Deal Roofing Services Ltd | Construction | 2025 |
| Fairprice Mobility Ltd | Wholesale & retail | 2012 |
| Faith Health Care Priority Ltd | Health & social care | 2022 |
| Falkirk and Kingdom Roofers Ltd | Construction | 2023 |
| Fanika Hairworld Ltd | Other services | 2026 |
| Fasttrac Design Limited | Professional & scientific | 2006 |
| Ferris Industrial Services Limited | Professional & scientific | 2014 |
| Ferris Tyre Recycling Limited | Water & waste | 2026 |
| Ffdr 2017 Ltd | Construction | 2017 |
| Ffdr Limited | Construction | 1998 |
| FG DAN Cars Ltd | Transport & logistics | 2023 |
| FIB Whisky Ltd. | Wholesale & retail | 2017 |
| Fido Treats Ltd | Wholesale & retail | 2024 |
| Fife Accident Repair Centre Limited | Wholesale & retail | 2004 |
| Fife Bathrooms Ltd | Construction | 2025 |
| Fife BIN Cleaning Services Limited | Administrative & support | 2017 |
| Fife Chamber of Commerce & Enterprise Limited | Public administration | 1999 |
| Fife CIC | Information & communication | 2026 |
| Fife Coast and Countryside Trust | Administrative & support | 2001 |
| Fife Communities Climate Action Network CIC | Other services | 2020 |
| Fife Community Interpreting Service | Professional & scientific | 2010 |