| Benchmark Space Systems UK Limited | Manufacturing | 2022 |
| Bennochy Bars Ltd | Hospitality | 2024 |
| Bervan Ltd | Hospitality | 2025 |
| Better Internet Search Ltd | Information & communication | 2019 |
| Betty Properties Limited | Households | 2018 |
| Bijan Habib Delivery Ltd | Transport & logistics | 2026 |
| Bilboats Limited | Wholesale & retail | 2022 |
| Bill Rolland Limited | Professional & scientific | 2013 |
| Billy Mitchell Electrical Ltd | Construction | 2021 |
| Bio-Tex Ltd | Manufacturing | 2025 |
| Birnie Property Limited | Real estate | 2025 |
| BJC Interiors Limited | Construction | 2012 |
| BKA Group (Scotland) Ltd | Wholesale & retail | 2025 |
| Blackbird Corporate Limited | Information & communication | 2013 |
| Bladefusion Ltd | Construction | 2025 |
| Blair Scaffolding (Scotland) Limited | Construction | 2023 |
| Blankenstein Welding Ltd | Manufacturing | 2018 |
| Bleeding Gums Wrestling Ltd | Manufacturing | 2025 |
| Blessing of Burntisland Limited Partnership | | 1994 |
| Blind Davy's Limited | Wholesale & retail | 2011 |
| Blue Oval Performance Limited | Wholesale & retail | 2020 |
| Blue Selkie Ltd | Education | 2024 |
| Blue Skies (Fife) Ltd | Real estate | 2017 |
| Blythy Reserve Ltd | Hospitality | 2025 |
| BM Carpets and Flooring Ltd | Wholesale & retail | 2025 |
| Bmcs (Scotland) Limited | Financial services | 2016 |
| Bobbys Snack Bar Limited | Hospitality | 2017 |
| Body Republic Limited | Administrative & support | 2011 |
| Bodywyse Autocare Limited | Wholesale & retail | 2005 |
| Book Nook Retreats Ltd | Hospitality | 2025 |
| BPK Engineering Ltd | Mining & quarrying | 2025 |
| Braehead Homes (Scotland) Limited | Construction | 2016 |
| Braehead Property Company Limited | Construction | 1986 |
| Braveheart MMA Ltd | Arts & recreation | 2025 |
| Braveheart Promotions Limited | Arts & recreation | 2025 |
| Braveheart Services Ltd | Administrative & support | 2020 |
| Bravion Solutions Limited | Professional & scientific | 2018 |
| Brew Begining Ltd. | Hospitality | 2024 |
| Bridan Design & Supply Ltd | Wholesale & retail | 2023 |
| Bridg-It Marketing Limited | Real estate | 1989 |
| Bridgeston Motors Fife Limited | Wholesale & retail | 2019 |
| Bright Red Publishing Limited | Information & communication | 2007 |
| Britex Traders Ltd | Manufacturing | 2026 |
| Bryer Cunningham Painting Services Ltd | Construction | 2023 |
| Bryson & Hare Limited | Other services | 2022 |
| BSB Petroleum Limited | Other services | 2019 |
| Bsound Limited | Administrative & support | 2008 |
| BSW Building Systems Ltd | Professional & scientific | 2008 |
| Bubbles Boutique Hair & Beauty Limited | Other services | 2017 |
| Build My Village Ltd | Information & communication | 2026 |
| Burghys Ltd | Hospitality | 2025 |
| Business Oxygen Limited | Professional & scientific | 2008 |
| Buy and Sell Cars Limited | Wholesale & retail | 2025 |
| Byrne Bikes Ltd | Manufacturing | 2026 |
| C and H Taxis Limited | Transport & logistics | 2018 |
| C Duncan Properties Ltd | Real estate | 2023 |
| C G Property Developments (Fife) Limited | Real estate | 2020 |
| C L Maxwell Limited | Professional & scientific | 2020 |
| C M Smith Holdings Ltd | Professional & scientific | 2024 |
| C Sinclair Fish Ltd | Wholesale & retail | 2020 |
| C Wands Joinery Ltd | Construction | 2019 |
| C&L Agri Ltd | Agriculture & fishing | 2026 |
| C.I Lettings Ltd | Real estate | 2021 |
| C2P Cleaning Services Ltd | Administrative & support | 2026 |
| C2P Limited | Administrative & support | 2024 |
| CA Cleaning Services Limited | Administrative & support | 2025 |
| Cabello Hair Limited | Other services | 2023 |
| Caduceus Care Group Ltd | Administrative & support | 2023 |
| Cafe Choca Latte Ltd | Hospitality | 2024 |
| Cafe Continental Ltd | Hospitality | 2024 |
| Cairn Corporate Ltd. | Financial services | 2011 |
| Cairn Independent Limited | Financial services | 1993 |
| Cairn Nexus Limited | Professional & scientific | 2011 |
| Caldicott & Henderson Ltd | Other services | 2025 |
| Caledon Property Partners Limited | Real estate | 2025 |
| Caledonia Fire and Security Limited | Administrative & support | 2003 |
| Caledonia Fire and Security Property Company Limited | Real estate | 2015 |
| Caledonia Signs Limited | Manufacturing | 1996 |
| Caledonian Roofing Scotland Limited | Construction | 2024 |
| Calforth Construction Ltd | Construction | 2022 |
| Calforth Developments Limited | Construction | 2024 |
| Callum Robertson Ltd | Professional & scientific | 2022 |
| Camilla Training Aspects Limited | Education | 2004 |
| Camryth Properties Limited | Real estate | 2025 |
| Candy Cloud Fife Ltd | Wholesale & retail | 2025 |
| Capercaillie Travel Limited | Administrative & support | 2001 |
| Careoligy Limited | Other services | 2018 |
| Carevisits Ltd | Information & communication | 2026 |
| CARGOO4SERVICE Limited | Transport & logistics | 2025 |
| Caribou Build Ltd | Construction | 2016 |
| Carleton Electrical Ltd | Construction | 2021 |
| Carly Imrie Performing Arts Ltd | Arts & recreation | 2024 |
| Carnegie Financial Services Ltd | Financial services | 2010 |
| Caroline Conglomerates Limited | Wholesale & retail | 2003 |
| Carpe Diem Marquees and Events Limited | Administrative & support | 2020 |
| Carriston Homes Limited | Construction | 2006 |
| Carrypour Drinkware Ltd | Manufacturing | 2026 |
| Cartmore MOT and Repair Centre Limited | Wholesale & retail | 2025 |
| Cartmore Pallets Glasgow Ltd | Manufacturing | 2023 |
| Caspian & Paradise Limited | Wholesale & retail | 2024 |