| Altinvst Ltd | Financial services | 2026 |
| Alva International Recruitment Ltd | Administrative & support | 2023 |
| Always Strive to Ltd | Arts & recreation | 2016 |
| AMA Postmail Limited | Transport & logistics | 2015 |
| AMB Paralegal Services Limited | Professional & scientific | 2025 |
| AMC Renewables Ltd | Professional & scientific | 2024 |
| AME Concepts Limited | Real estate | 2024 |
| Ammarify UK Ltd | Wholesale & retail | 2025 |
| AN Gordonach Ltd | Information & communication | 2023 |
| Anchor Timber Buildings Ltd | Wholesale & retail | 2020 |
| Andrew James Interiors Ltd | Manufacturing | 2020 |
| Andrew Kellet Training Limited | Professional & scientific | 2016 |
| Angel Automations Ltd | Hospitality | 2025 |
| Angel Massage Limited | Other services | 2025 |
| Anna Delivery Ltd | Transport & logistics | 2023 |
| Anstruther Pleasure Cruises Limited | Transport & logistics | 2005 |
| Anwar Consultancy Ltd | Administrative & support | 2025 |
| Anywall Ltd | Construction | 2012 |
| Aperti Ltd | Administrative & support | 2025 |
| Apex Roofing Solutions 247 Ltd | Construction | 2026 |
| Apollo Fife Limited | Wholesale & retail | 2017 |
| Aquati Ltd | Wholesale & retail | 2016 |
| Aram (Fife) Limited | Other services | 2016 |
| ARB Tech (Fife) Ltd | Administrative & support | 2022 |
| Archas Cultural Heritage Ltd | Professional & scientific | 2011 |
| Ardbrecknish Properties Limited | Hospitality | 2025 |
| Ardglen Holdings Ltd | Hospitality | 2026 |
| Ardrtrading Ltd | Wholesale & retail | 2019 |
| Arduaine Limited | Professional & scientific | 2017 |
| Arena Club Limited | Financial services | 2025 |
| Arena Gym Fife Limited | Other services | 2018 |
| Arid Communication Systems Ltd | Information & communication | 2020 |
| ARK Access Limited | Construction | 2020 |
| Armada Projects Ltd | Construction | 2016 |
| Aron Cleaning Services Ltd | Administrative & support | 2025 |
| Arran Collins Roofing Ltd | Construction | 2025 |
| Arro Lighting and Events Ltd | Wholesale & retail | 2014 |
| Arrows Property Development Limited | Construction | 2026 |
| Artisan Fridays Ltd | Wholesale & retail | 2016 |
| Arts (ASH RAD Teleradiology Services) Ltd | Health & social care | 2025 |
| ASA Investments Fife Ltd | Financial services | 2026 |
| ASC TEC Ltd | Wholesale & retail | 2025 |
| ASH Scot Ltd | Wholesale & retail | 2024 |
| Ashton and Murray Holdings Ltd | Construction | 2021 |
| Asif & Sons Properties Limited | Real estate | 2023 |
| ASK Install Services Limited | Construction | 2026 |
| Aspire Event Hire Limited | Administrative & support | 2025 |
| Assisted Services (Fife) Limited | Health & social care | 2022 |
| Assure Start Ltd | Information & communication | 2024 |
| Assured Management Consultancy Limited | Professional & scientific | 2009 |
| Assured Management Group Limited | Professional & scientific | 2009 |
| Assured Property Consultants Limited | Professional & scientific | 2009 |
| Astrak (UK) Limited | Wholesale & retail | 2013 |
| Astrak Group Limited | Wholesale & retail | 2014 |
| Asylum Sound Productions Ltd | Information & communication | 2020 |
| ATL Real Estate Ltd | Real estate | 2021 |
| Auchtertool Community Trust | Other services | 2017 |
| Auto-Bil Trailers Ltd | Wholesale & retail | 2017 |
| Autobil Trailers Dybowski Limited | Wholesale & retail | 2020 |
| Automotive Spares and Repairs Limited | Wholesale & retail | 2021 |
| Auzora Ltd | Wholesale & retail | 2021 |
| Avarus Limited | Hospitality | 2017 |
| Avenue Scotland Group Limited | Administrative & support | 2017 |
| Avicenna Care Limited | Health & social care | 2017 |
| AW GAS Repairs Ltd | Construction | 2020 |
| AW Kitchen Fitters (Fife) Ltd | Construction | 2023 |
| Aytoun Works Ltd | Professional & scientific | 2021 |
| B & L Properties (Fife) Limited | Real estate | 2018 |
| BA Quality Inspection Ltd | Construction | 2023 |
| Babyland Fife Ltd. | Wholesale & retail | 2013 |
| Backman Clinic Limited | Health & social care | 2026 |
| Bagels & Co (UK) Ltd | Hospitality | 2019 |
| Bain Property Group Ltd | Real estate | 2023 |
| Baisley Ltd | Transport & logistics | 2026 |
| Balbeggie Steel Structures Limited | Manufacturing | 2005 |
| Balbirnie Developments Ltd | Real estate | 2016 |
| Balfarg Properties Ltd | Construction | 2021 |
| Balgonie Consulting Limited | Wholesale & retail | 2025 |
| Balgonie Motor Repairs Ltd | Wholesale & retail | 2020 |
| Balmoral Construction Ltd | Construction | 2025 |
| Balwearie Gymnastics Club Ltd | Arts & recreation | 2013 |
| Bar Refrigeration Ltd | Manufacturing | 2007 |
| Baracoa 1984 Ltd | Transport & logistics | 2025 |
| Barry Fimister Autotint Limited | Wholesale & retail | 2011 |
| Bartys Ltd | Extraterritorial | 2023 |
| Bash Maintenance Contracts Ltd | Real estate | 2022 |
| Bblmax Ltd | Professional & scientific | 2023 |
| BCS (Fife) Trading AS Bridges Cleaning Services Ltd | Administrative & support | 2020 |
| BDR Bathroom Innovation Ltd | Construction | 2026 |
| Beans Bubbles and Beers Limited | Wholesale & retail | 2020 |
| Beauhaus UK Ltd | Real estate | 2018 |
| BEE Mortgage Wise Ltd | Financial services | 2020 |
| Bee-Laced Ltd | Wholesale & retail | 2026 |
| Bell Haulage & Metal Merchants Limited | Wholesale & retail | 2025 |
| Bellnell Holdings Ltd | Real estate | 2023 |
| Bellnell Properties Ltd | Real estate | 2023 |
| Bellshelf (One) Limited | Administrative & support | 1991 |
| BEN Murdoch Delivery Ltd | Transport & logistics | 2026 |
| Benarty Construction Contracts Ltd | Construction | 2010 |
| Benchmark Joinery & Building Contractors Ltd | Construction | 2024 |