| 0307 Limited | Hospitality | 2025 |
| 1304 Investments Ltd | Extraterritorial | 2023 |
| 21 Degrees Plumbing Limited | Construction | 2019 |
| 21 Two Property Company Limited | Real estate | 2025 |
| 212 Pizza Co. Ltd | Hospitality | 2025 |
| 2AM Creations Limited | Information & communication | 2014 |
| 3D Precision Engineering Limited | Professional & scientific | 2005 |
| 414 Alcohols Limited | Manufacturing | 2016 |
| 4CONSULTING Limited | Professional & scientific | 2001 |
| 650G Ltd | Real estate | 2015 |
| 786 Estates Limited | Real estate | 2007 |
| A & KB Properties Ltd | Real estate | 2024 |
| A & L Property (Fife) Ltd | Real estate | 2025 |
| A and C Audio Visual Limited | Wholesale & retail | 2003 |
| A H Garage Services Limited | Wholesale & retail | 2022 |
| A Stevenson Services Limited | Professional & scientific | 2024 |
| A&J Properties Fife Ltd | Real estate | 2024 |
| A-One Care Limited | Health & social care | 2026 |
| A. Dawson Roofing Services Ltd | Construction | 2020 |
| A.D Aesthetics Fife Ltd | Other services | 2021 |
| A.L.C Engineering & Industrial Services Ltd | Manufacturing | 2022 |
| A.M. Decor Limited | Construction | 2017 |
| A.N. Electrics Limited | Construction | 2013 |
| A.T.Rolland (Fife) Ltd. | Administrative & support | 2010 |
| A.V.S. Designs Ltd | Professional & scientific | 2022 |
| A1 Cleaning Services Fife Ltd | Administrative & support | 2016 |
| A2Z Builders Ltd | Construction | 2024 |
| A2Z Builders Scotlands Ltd | Construction | 2026 |
| AA Electrical and Renewable Services Ltd | Construction | 2022 |
| Abbeyfield Societies in Scotland Ltd | Health & social care | 2012 |
| Abbotsford Care (Glenrothes) Limited | Health & social care | 2009 |
| Abbotsford Property (Dunfermline) Limited | Professional & scientific | 2011 |
| Abeltek Ltd | Professional & scientific | 2026 |
| Aberdeen Homes Ltd | Construction | 2024 |
| Aberdeenshire Homes Limited | Construction | 2025 |
| AD Services Courier Limited | Other services | 2019 |
| Ad&Ko Logistic Ltd | Transport & logistics | 2025 |
| Adaj Engineering Ltd | Professional & scientific | 2021 |
| Adaj Supplies Ltd | Wholesale & retail | 2023 |
| Adam Scally VPR Limited | Construction | 2026 |
| Adamila Ltd | Wholesale & retail | 2025 |
| Adamson Writes Ltd | Professional & scientific | 2024 |
| Addon Developments Limited | Construction | 2018 |
| Admo Property Ltd | Real estate | 2024 |
| Advanced Recruitment Solutions Limited | Administrative & support | 2005 |
| ADZ Global Limited | Administrative & support | 2018 |
| Ae&H Ltd | Wholesale & retail | 2025 |
| AEW Imaging Ltd | Health & social care | 2024 |
| Affinex Internationals Ltd | Information & communication | 2025 |
| Affordable Housing Scotland Limited | Construction | 2018 |
| AG Care Ltd | Health & social care | 2025 |
| AG Temple Investments Limited | Real estate | 2019 |
| Agak Biologics Ltd | Information & communication | 2021 |
| AGC Legal Services Limited | Professional & scientific | 2019 |
| Ageless Companions Limited | Health & social care | 2019 |
| Agk’S Mobility Ltd | Transport & logistics | 2025 |
| AGM & Sons Properties Ltd | Construction | 2024 |
| AHJ Ltd | Real estate | 2024 |
| Ahmed Brothers Foods Ltd | Hospitality | 2026 |
| Aiarra Ltd | Professional & scientific | 2024 |
| Airtech Solutions Ltd | Professional & scientific | 2024 |
| Aiwan Barbers Ltd | Other services | 2026 |
| AJ Auctions Fife Ltd | Wholesale & retail | 2018 |
| AJ Concrete Solutions Ltd | Construction | 2025 |
| AJ Investing Ltd | Real estate | 2025 |
| AJ Trade Global Ltd | Wholesale & retail | 2026 |
| AJB Design Services Limited | Professional & scientific | 1989 |
| Ajbass IT Ltd | Information & communication | 2026 |
| AJC Lettings Limited | Real estate | 2022 |
| AJL Electrical Services Ltd | Construction | 2025 |
| AK Frames Ltd | Manufacturing | 2019 |
| AK187 Ltd | Professional & scientific | 2015 |
| Akaw Limited | Professional & scientific | 2019 |
| AL Fajar Building Maintenance UK Limited | Construction | 2019 |
| AL Shaan Limited | Wholesale & retail | 2016 |
| Al-Falah Stores Ltd | Wholesale & retail | 2021 |
| Al-Rehman FM Limited | Administrative & support | 2019 |
| Alan Duffus Limited | Wholesale & retail | 1976 |
| Alan Hodge Joiners Ltd | Construction | 2018 |
| Alan Santry Limited | Wholesale & retail | 2008 |
| Alanya Brothers Ltd | Hospitality | 2018 |
| Alator Consultants Ltd | Professional & scientific | 2014 |
| Alayia Stores Ltd | Wholesale & retail | 2026 |
| Alba Export Agencies Ltd | Other services | 2006 |
| Albavet Limited | Professional & scientific | 2004 |
| Albion Investments Limited | Real estate | 1998 |
| Aldea Property Limited | Professional & scientific | 1994 |
| Alexander Simpson Kitchens Ltd | Construction | 2022 |
| Alexdomelle Solutions Ltd | Hospitality | 2025 |
| Alexeve Access Ltd | Construction | 2025 |
| Alford Garage Limited | Wholesale & retail | 2001 |
| ALG Coatings Ltd | Construction | 2020 |
| ALI Import Export Limited | Wholesale & retail | 2019 |
| Alikat Ltd | Education | 2023 |
| ALK IRE Limited | Hospitality | 2025 |
| ALL Cuisines Catering Ltd | Hospitality | 2025 |
| Alleco Contracts Ltd | Energy | 2017 |
| Almaz Innovate Fife Foods Company Limited | Manufacturing | 2018 |
| Alsi Subsea Operations Ltd | Mining & quarrying | 2019 |
| Alternative Access Services Ltd | Construction | 2019 |