| W50 Consultancy Ltd | Professional & scientific | 2022 |
| Walker & Templeton Killi Limited | Wholesale & retail | 2021 |
| Walker Gordon Associates Ltd | Professional & scientific | 2017 |
| Walkers 101 Services Ltd | Construction | 2020 |
| Wallace Gilbert Joiners Ltd | Construction | 2018 |
| Wallace Land & Sea Services Ltd | Transport & logistics | 2015 |
| Wallace Treecare Ltd. | Agriculture & fishing | 2009 |
| Warranty International Ltd | Transport & logistics | 2015 |
| Warriech Real Estate Ltd | Real estate | 2025 |
| Warrior Scotland Limited | Construction | 2000 |
| Warrior Software Ltd | Information & communication | 2024 |
| Warriors Studio Ltd | Professional & scientific | 2014 |
| Watches From Switzerland Limited | Wholesale & retail | 2020 |
| Watson Inchbean Limited | Real estate | 2026 |
| Waveform Collective Limited | Information & communication | 2026 |
| WCB Group Ltd | Administrative & support | 2024 |
| WCF Caravans Limited | Real estate | 2021 |
| WCK Windows & Doors (Ayrshire) Ltd | Construction | 2022 |
| Wcts Property Group Limited | Real estate | 2024 |
| WE DO Safety Limited | Construction | 2019 |
| Webb Joinery Ltd | Construction | 2017 |
| Webb Windows & Doors Ltd | Manufacturing | 2025 |
| Weddings & Special Events by Louize Ltd | Other services | 2022 |
| WEE Black Beastie Ltd | Information & communication | 2018 |
| Weir & Co. Holdings Ltd | Manufacturing | 2024 |
| Wellbeck Kilmarnock Ltd | Hospitality | 2026 |
| Wellington Properties (Kilmarnock) Limited | Construction | 2021 |
| Wellpark Roofing Ltd | Construction | 2019 |
| West Coast Events Limited | Other services | 2025 |
| West Coast Foods (Prestwick) Limited | Wholesale & retail | 2014 |
| West Coast Stonemasons Ltd | Manufacturing | 2024 |
| West Coast Timber Frame Limited | Construction | 2020 |
| West Coast Tree Surgeons Limited | Arts & recreation | 2021 |
| West George Ltd | Hospitality | 2019 |
| West George ST. Ltd | Real estate | 2019 |
| West of Scotland Loan Fund | Financial services | 1996 |
| Western Meeting Club 2007 Holdings Limited | Financial services | 2007 |
| Western Meeting Club 2007 Limited | Financial services | 2007 |
| Westfield Partners Limited | Financial services | 2024 |
| Westgale Holdings Limited | Real estate | 2016 |
| Westlaigh Ltd | Hospitality | 2024 |
| Westland Signs & Plastics Limited | Information & communication | 2023 |
| Westlin Construction Ltd | Construction | 2014 |
| Westlin Group Ltd | Construction | 2014 |
| Westlin Rail Ltd | Construction | 2014 |
| Westray Services Limited | Construction | 2016 |
| Wheelie School Limited | Education | 2021 |
| Whiteford Contracts Limited | Construction | 2005 |
| Whitehorse PUB Village Ltd | Hospitality | 2023 |
| Whole Shebang Education Limited | Education | 2025 |
| Wholesale Italian Ltd | Wholesale & retail | 2020 |
| Wilcam Limited | Professional & scientific | 2019 |
| Wild Wood by Bruce Ltd | Manufacturing | 2021 |
| William Clegg Ltd | Transport & logistics | 2014 |
| William Harvie & Co. (Farmers) Limited | Agriculture & fishing | 1950 |
| William Lambie Limited | Agriculture & fishing | 1957 |
| William Morton Properties Limited | Real estate | 1993 |
| William Smith Engineering Limited | Construction | 2021 |
| William Wilsons FRS Valves Limited | Manufacturing | 2019 |
| William Young (Skerrington Mains) Limited | Agriculture & fishing | 1949 |
| Willow Tree Aesthetics Limited | Health & social care | 2021 |
| Wilson Commercial Consulting Ltd | Professional & scientific | 2022 |
| Wilson Homes (Kilmarnock) Limited | Construction | 1984 |
| Wilson R Holdings Ltd | Real estate | 2026 |
| Wilsons Tavern Ltd | Hospitality | 2025 |
| Wilsonskitchenpantryayrshire Ltd | Hospitality | 2026 |
| WIN Together Ltd | Professional & scientific | 2017 |
| Window Buff Ltd | Administrative & support | 2019 |
| Winterskloof Limited | Extraterritorial | 2024 |
| Wishing Wellness Ltd | Education | 2022 |
| With Love Cookies Ltd | Manufacturing | 2024 |
| Wiwa Limited | Arts & recreation | 2022 |
| WJ Smith Business Finance Ltd | Financial services | 2024 |
| Wjhh Services Limited | Construction | 2014 |
| WK Contracts Limited | Construction | 2015 |
| Wmbb Scotland Ltd | Hospitality | 2016 |
| Womble Development Ltd. | Information & communication | 2018 |
| Woods Associates Surveyors Ltd. | Administrative & support | 2000 |
| Woods Ceilings & Partitions Ltd | Construction | 2019 |
| Woody Block Limited | Energy | 2017 |
| Woodys 18 Limited | Wholesale & retail | 2022 |
| Woolfsons of James Street Limited | Wholesale & retail | 1983 |
| Working for Scotland Limited | Professional & scientific | 2004 |
| World Donner Ltd | Hospitality | 2021 |
| World Food Market Limited | Hospitality | 2026 |
| Wowe Ltd | Health & social care | 2023 |
| WP Commercial Property Limited | Real estate | 2017 |
| Wrap & Roll Fresh Food Ltd | Hospitality | 2024 |
| WRB Brickwork and Construction Ltd | Construction | 2021 |
| Wreckansignalling Ltd | Construction | 2025 |
| Wren Beauty Ltd | Other services | 2025 |
| Wright Assets Ltd | Financial services | 2024 |
| Writer's Science Ltd | Information & communication | 2025 |
| WVT Tender Solutions Limited | Real estate | 2025 |
| Wynd Bistro Ltd | Hospitality | 2024 |
| XEL Business Solutions Ltd | Professional & scientific | 2014 |
| Yehorov Media Ltd | Information & communication | 2026 |
| YJ Medical Services Limited | Health & social care | 2014 |
| YJ Properties Ltd | Real estate | 2024 |
| Youblawg Limited | Information & communication | 2012 |