| TIME4PAWS Ltd | Agriculture & fishing | 2022 |
| Tipperty Properties Limited | Transport & logistics | 2025 |
| TK Templates Limited | Professional & scientific | 2025 |
| TKF Engineering Limited | Professional & scientific | 2014 |
| TKG Prestwick Ltd | Wholesale & retail | 2020 |
| Tkmb Ltd | Financial services | 2014 |
| TMR Business Services Ltd | Professional & scientific | 2014 |
| Tobermory Landscapes Ltd | Administrative & support | 2018 |
| Tocopa Limited | Arts & recreation | 2014 |
| Together Leading Communities Ltd | Professional & scientific | 2023 |
| TOM Floyd & SON Ltd | Agriculture & fishing | 2023 |
| Topmark Holdings Limited | Education | 2018 |
| Tower Group Holdings Limited | Professional & scientific | 2015 |
| Tower House Craufurdland Limited | Hospitality | 2013 |
| Townhead of Hairshaw Farm Ltd | Agriculture & fishing | 2026 |
| TP Data Installations Limited | Manufacturing | 2018 |
| TPS Weldtech Limited | Manufacturing | 1973 |
| Track Civil Engineering Solutions Ltd | Construction | 2022 |
| Tracol Ltd | Information & communication | 2010 |
| Trade Leads Ltd | Professional & scientific | 2025 |
| Trade the London Limited | Wholesale & retail | 2025 |
| Training First Safety Ltd | Education | 2019 |
| Transcool (Scotland) Ltd. | Wholesale & retail | 2007 |
| Transform Alloys Scotland Ltd | Wholesale & retail | 2021 |
| Transition of Character Ltd | Financial services | 2011 |
| TREE3CRAFTS Ltd | Manufacturing | 2023 |
| Treebase Windows Limited | Construction | 1987 |
| Treefront 365 Limited | Professional & scientific | 2023 |
| TRG Advantage Limited | Information & communication | 2002 |
| TRG Makeup Ltd | Other services | 2020 |
| Trinity AYR Ltd | Real estate | 2021 |
| Trinity Irvine Ltd | Real estate | 2024 |
| Trinity Kilmarnock Ltd | Real estate | 2020 |
| Truereflectionmemorials Ltd | Manufacturing | 2025 |
| Truheat Services Ayrshire Ltd | Construction | 2025 |
| Truth 9 Ltd | Hospitality | 2020 |
| Tsac Kilmarnock Ltd | Health & social care | 2025 |
| Tspaces Limited | Hospitality | 2015 |
| TSR (Scotland) Ltd. | Other services | 2014 |
| Tuartsee Ltd | Agriculture & fishing | 2025 |
| Tulia Blueclay Limited | Professional & scientific | 2022 |
| Tulsee Eyecare Ltd | Health & social care | 2022 |
| Turf at the Top Ltd | Administrative & support | 2018 |
| Tutoring Scotland Ltd | Education | 2023 |
| TUX Software Ltd | Information & communication | 2026 |
| TV Local (Scotland) Limited | Information & communication | 2010 |
| Twenty Fore Golf Studio Limited | Arts & recreation | 2026 |
| TWENTY1FORE Ltd | Real estate | 2025 |
| Twfs Catering Ltd | Hospitality | 2022 |
| Tynedale Investments Limited | Real estate | 2021 |
| UA Global Trading Limited | Wholesale & retail | 2025 |
| Ucan Enterprises Ltd | Administrative & support | 2012 |
| Ucan Learn.Work.Live Ltd | Education | 2012 |
| UK Nails & Beauty JR Ltd | Other services | 2024 |
| UK Prosecures Ltd | Administrative & support | 2020 |
| Ultimate Restoration Ltd | Administrative & support | 2026 |
| UMR Shawarma Ltd | Hospitality | 2025 |
| Underground Scotland Ltd | Hospitality | 2023 |
| Underground Utilities Limited | Construction | 2024 |
| Underwood Life Sciences Limited | Professional & scientific | 2018 |
| Uniq Interiors Ltd | Construction | 2026 |
| Universal Medicare Pro and Student Advisory Limited | Health & social care | 2022 |
| UP 4 LOO Hire Limited | Administrative & support | 2019 |
| Uplands Ayrshire Limited | Hospitality | 2017 |
| Urban Furniture Ltd. | Wholesale & retail | 2005 |
| Urban Studios (Ayrshire) Ltd | Education | 2024 |
| USA Nails 68 Ltd | Other services | 2024 |
| Usave Kilmarnock Ltd | Wholesale & retail | 2017 |
| Usave Symington Ltd | Wholesale & retail | 2026 |
| Utopia Repair Centre Limited | Real estate | 2023 |
| V Store Ltd | Administrative & support | 2019 |
| Valley Joiners Ltd | Construction | 2021 |
| Valley Parcel Services Limited | Transport & logistics | 2020 |
| Valleycraft (Scotland) Ltd. | Construction | 2004 |
| Van Transform Ltd. | Manufacturing | 2011 |
| Vanconvert Ltd | Wholesale & retail | 2015 |
| Vanguard Total Property Solutions Ltd | Construction | 2026 |
| Vanquish Van Solutions Ltd | Manufacturing | 2024 |
| Vantage Acquisitions Ltd | Real estate | 2026 |
| Vehicle Resolutions Ltd | Professional & scientific | 2015 |
| Velocity Asset Finance Ltd | Financial services | 2024 |
| Venture Entertainment Limited | Information & communication | 2004 |
| Venus Salon Enterprises Ltd | Other services | 2021 |
| Verfico Limited | Information & communication | 2013 |
| Veroxuk Ltd | Hospitality | 2025 |
| VG Nvto Developments Limited | Administrative & support | 2012 |
| VG Windvestments Limited | Administrative & support | 2016 |
| Virtual Facilities Services Ltd | Administrative & support | 2016 |
| Virtual Water Services Ltd | Professional & scientific | 2020 |
| Vita Boutique Limited | Wholesale & retail | 2013 |
| Vivehr Ltd | Administrative & support | 2019 |
| VON and RIO Pokemon Centre Ltd | Wholesale & retail | 2026 |
| VRA Enterprises Limited | Hospitality | 2014 |
| VSE Consult Ltd | Professional & scientific | 2025 |
| VSE Group Ltd | Financial services | 2019 |
| W & W Roughcasting, Roofing and General Builders Ltd | Construction | 2025 |
| W J Mccrone Limited | Wholesale & retail | 2008 |
| W Milligan Roofing Limited | Construction | 2008 |
| W Walker Decorators Ltd | Construction | 2015 |
| W. W. Wales Limited | Wholesale & retail | 1983 |