| Elanic (Holdings) Ltd | Financial services | 2019 |
| Elanic Ltd. | Health & social care | 2009 |
| Elba Financial Services Ltd | Financial services | 2025 |
| Elddis Homes Limited | Construction | 2015 |
| Elder Properties Group Ltd | Real estate | 2025 |
| Elite Financial Advice Ltd | Financial services | 2018 |
| Elite Financial Group Ltd | Professional & scientific | 2020 |
| Elite Fitness Ayrshire Ltd | Arts & recreation | 2023 |
| Elite Food Express Ltd | Hospitality | 2022 |
| Elite Glazing Installations Ltd | Construction | 2016 |
| Elite Joinery & Builders Limited | Construction | 2011 |
| Elite Property Management (Scotland) Limited | Extraterritorial | 2012 |
| Elizabeth G Charters Limited | Transport & logistics | 2011 |
| Elizabeth Wallace Bridal Limited | Other services | 2004 |
| Elkis Ltd | Wholesale & retail | 2013 |
| Elliot Property Limited | Real estate | 2022 |
| Elliot Residential Limited | Real estate | 2023 |
| Elmar Joinery Limited | Construction | 2009 |
| Eloura Homes Ltd | Households | 2025 |
| Elyfem Enterprises Ltd | Wholesale & retail | 2025 |
| EMB Contracting Limited | Professional & scientific | 2013 |
| Ember Energy Renewables Limited | Administrative & support | 2020 |
| Ember Pilates Studio Limited | Arts & recreation | 2026 |
| Emblem Packaging Limited | Financial services | 2025 |
| Embracesphere Ltd | Education | 2024 |
| Emilew Ltd | Arts & recreation | 2026 |
| Emilymill Kennels Limited | Other services | 2019 |
| Emirgreen Limited | Financial services | 2026 |
| Emma FM Limited | Wholesale & retail | 2020 |
| Emma's Aesthetics and Skincare Ltd | Other services | 2023 |
| Enchanted Spaces Ltd | Wholesale & retail | 2025 |
| Energise Zone LLP | | 2013 |
| Energy Surveys Scotland Ltd | Professional & scientific | 2023 |
| Energy Train Together Ltd | Arts & recreation | 2025 |
| Engebret Limited | Wholesale & retail | 1957 |
| Engraving Services AYR Limited | Professional & scientific | 2011 |
| Enhance Hospitality Limited | Professional & scientific | 2011 |
| Enigma Controls Ltd | Education | 2025 |
| Enrolpay Limited | Information & communication | 2013 |
| Enterprising Transition Care Ayrshire C.I.C. | Public administration | 2022 |
| Envirosmart Limited | Construction | 2020 |
| Enzo Drywall Solutions Ltd | Construction | 2020 |
| Epic Competitions Ltd | Other services | 2023 |
| Equine Therapy UK Ltd | Health & social care | 2015 |
| ER Medical Training & Events Scotland Ltd | Health & social care | 2026 |
| Erimeg Solutions Limited | Professional & scientific | 2024 |
| Eris Consulting Ltd | Professional & scientific | 2025 |
| Espresso Kart Collective Limited | Hospitality | 2025 |
| Essen Properties Limited | Real estate | 2014 |
| EST Properties Limited | Real estate | 2006 |
| Ethereal Luxury Body Piercing Limited | Other services | 2025 |
| Etive Studios Ltd | Information & communication | 2023 |
| Euro-Range Ltd. | Wholesale & retail | 2004 |
| Evensong Ltd | Information & communication | 2017 |
| Everest HEA Ltd | Real estate | 2025 |
| EVO Chargers Ltd | Construction | 2021 |
| Evolution Custom Cycles Ltd | Wholesale & retail | 2009 |
| Evolution Fitness & Leisure Ltd | Financial services | 2019 |
| Evolution Gym Kilmarnock Ltd | Administrative & support | 2023 |
| Evra Group Limited | Construction | 2023 |
| EVT (Scotland) Ltd | Transport & logistics | 2021 |
| Exceedingly Good Investments Ltd | Real estate | 2016 |
| Excellence Driven Drilling Solutions Ltd | Professional & scientific | 2017 |
| Excelsior HR Limited | Professional & scientific | 2011 |
| EXM Ltd | Professional & scientific | 2017 |
| Express Landscaping & Gardening Ltd | Administrative & support | 2026 |
| External Consultancy Group Limited | Construction | 2015 |
| Extm Ltd | Professional & scientific | 2020 |
| Ezilogic Limited | Professional & scientific | 2007 |
| Ezoz Limited | Professional & scientific | 2017 |
| F & K Holiday Properties Limited | Hospitality | 2012 |
| F J Robin Property Investments Ltd | Real estate | 2023 |
| Fairlie Woodfuels (Scotland) Limited | Wholesale & retail | 2025 |
| Fairway Utilities Ltd | Construction | 2024 |
| Fairways Sportsgrounds Limited | Administrative & support | 2007 |
| Fast Sheep Ltd | Professional & scientific | 2018 |
| FAT Tractors Ltd | Manufacturing | 2019 |
| FB Concepts Ltd | Real estate | 2022 |
| FED by Farmers Ltd | Wholesale & retail | 2023 |
| Feeling and Focus Ltd | Information & communication | 2011 |
| Feeling Fabulous (Ayrshire) Ltd | Other services | 2025 |
| Fence Squad Ayrshire Ltd | Administrative & support | 2024 |
| Fencerite Repairs Ltd | Construction | 2021 |
| Fencing & Decking Specialist's Ltd | Construction | 2026 |
| Ferguslea Motors Ltd | Wholesale & retail | 2017 |
| FG MED Limited | Extraterritorial | 2012 |
| FHD Ltd. | Real estate | 2008 |
| Fibre. Gradu Ltd | Construction | 2020 |
| Fibrecon Ltd | Information & communication | 2021 |
| File Transport Ltd | Wholesale & retail | 2025 |
| Finaap Ltd | Information & communication | 2025 |
| Fiona Halliday Optometry Ltd | Health & social care | 2025 |
| Fior Living Limited | Manufacturing | 2018 |
| Fire Door Inspection Services Ltd | Public administration | 2022 |
| Firefly Joinery Services Ltd | Construction | 2024 |
| First Class Joinery Ltd | Construction | 2010 |
| First Insight Properties Ltd | Real estate | 2025 |
| FIS Consultancy Ltd | Professional & scientific | 2024 |
| Fisher Residential Property Ltd. | Real estate | 2021 |
| Fit Body Farm Ltd | Arts & recreation | 2015 |