| DMI Contracts Limited | Manufacturing | 2025 |
| DMJ Heating & GAS Services Limited | Construction | 2021 |
| Dobson Research & Development Limited | Professional & scientific | 2015 |
| Domtek Limited | Wholesale & retail | 2020 |
| Don'T BE a Starving Artist Ltd | Education | 2024 |
| Donaldsons Meat Ltd | Wholesale & retail | 2022 |
| Doncast Rendering Ltd | Construction | 2020 |
| Dontstressthedress Ltd | Other services | 2018 |
| Doonside Holdings Ltd | Financial services | 2018 |
| Dora Rose Enterprises Ltd | Health & social care | 2025 |
| Douglas Learner Drivers Limited | Other services | 2025 |
| Douglas Reid Ltd | Professional & scientific | 2012 |
| Douglas Wragg Electricians Limited | Construction | 2022 |
| Down to Earth Advice Limited | Professional & scientific | 2013 |
| Downie & Mcivor Capital Limited | Real estate | 2023 |
| Downie Advisory Limited | Professional & scientific | 2024 |
| Downie Capital Developments Ltd | Construction | 2019 |
| Downie Capital Limited | Professional & scientific | 2012 |
| Downie Capital Properties Limited | Real estate | 2017 |
| DR Ailey Mcallister Aesthetics Ltd | Health & social care | 2024 |
| DR Cyrus Wilson Limited | Health & social care | 2013 |
| DR Dundonald Offices Ltd | Real estate | 2025 |
| DR Dundonald Warehouse Ltd | Real estate | 2025 |
| DR Heather Watson Ltd | Health & social care | 2015 |
| Dressed 2 Kilmarnock Limited | Wholesale & retail | 2023 |
| Drivers Connect Ltd | Administrative & support | 2026 |
| DRM Works Limited | Wholesale & retail | 2022 |
| Drone Security Solutions Ltd | Other services | 2021 |
| DS Medical Ltd | Health & social care | 2024 |
| DSA Accommodation Ltd | Hospitality | 2024 |
| Dsaz Holdings Ltd | Financial services | 2018 |
| DSM Travel Limited | Transport & logistics | 2025 |
| Dual Fitness Ltd | Arts & recreation | 2016 |
| Duckys Catering Limited | Hospitality | 2025 |
| Ducky’S Kitchen Limited | Hospitality | 2022 |
| Ductwork Supplies Limited | Wholesale & retail | 2019 |
| Dulai Fuels Limited | Wholesale & retail | 2002 |
| Dumb Blonde Hair Extensions Ltd | Other services | 2018 |
| Duncail Property Ltd | Real estate | 2025 |
| Duncan & Laird Associates Ltd | Administrative & support | 2015 |
| Dunlop & District Community Company | Arts & recreation | 2002 |
| Dunlop & Gilhooley Limited | Real estate | 2019 |
| Dunlop and Lugton Park Association | Arts & recreation | 2004 |
| Dunlop Community Hub Limited | Hospitality | 2017 |
| Dunure Property Limited | Real estate | 2020 |
| Dust Fairy Limited | Administrative & support | 2018 |
| DVD Overstocks Ltd. | Wholesale & retail | 2013 |
| DWR Electrical Limited | Construction | 2012 |
| DY Civils Limited | Construction | 2022 |
| Dykefield Contracts Limited | Construction | 2014 |
| Dynamic Assessment UK Ltd | Education | 2019 |
| Dynamic Diamond Ltd | Health & social care | 2018 |
| Dynamite Dance Studios Ltd | Arts & recreation | 2022 |
| Dynamite Events & Costumes Ltd | Arts & recreation | 2022 |
| E J Templeton Limited | Manufacturing | 2012 |
| E M Young Limited | Wholesale & retail | 2014 |
| E P Rawding Limited | Professional & scientific | 2023 |
| E&A Creations Ltd | Wholesale & retail | 2026 |
| E.R Medical Events & Training Ltd | Health & social care | 2023 |
| E.Swan Aesthetics Ltd | Other services | 2023 |
| EA Healthcare Solutions Ltd | Education | 2026 |
| Eagle EYE Measurement Services Limited | Professional & scientific | 2022 |
| Eaglecrest Ltd | Real estate | 2022 |
| East Ayrshire Car Club Limited | Arts & recreation | 1972 |
| East Ayrshire Carers Centre | Other services | 2004 |
| East Ayrshire Citizens Advice Bureau | Information & communication | 1999 |
| East Ayrshire Leisure (Trading) C.I.C. | Hospitality | 2026 |
| East Ayrshire Women's AID | Administrative & support | 2011 |
| Easton Group Limited | Financial services | 2021 |
| Easton Housing Limited | Real estate | 2016 |
| Easton Property Auchans Limited | Real estate | 2016 |
| Easton Property Ayrshire Limited | Real estate | 2016 |
| Easton Property Kyle Limited | Real estate | 2026 |
| Easton Property Limited | Construction | 1996 |
| Easton Property Merkland Limited | Real estate | 2016 |
| Easton Property Newfield Limited | Real estate | 2016 |
| Easton Property Residential Limited | Real estate | 2016 |
| Easton Richmond Ltd | Real estate | 2021 |
| Easylift Crane Hire Ltd | Construction | 2020 |
| Eazy Freight Ltd | Transport & logistics | 2019 |
| Ebble Valley Consultancy Ltd | Professional & scientific | 2014 |
| EBK Trading Limited | Wholesale & retail | 2021 |
| Ebox Holdings Limited | Professional & scientific | 2000 |
| EBR Health Limited | Health & social care | 2022 |
| EBR Property Limited | Real estate | 2023 |
| Ebuzz Limited | Wholesale & retail | 2014 |
| EC Trading Ltd | Other services | 2026 |
| Echo Wellness Ltd | Professional & scientific | 2026 |
| Ecosse Aluminium Ltd | Manufacturing | 2021 |
| ED Properties Scotland Ltd | Real estate | 2024 |
| EDCHRIS26 Ltd | Professional & scientific | 2026 |
| Edward Minto & SON Limited | Administrative & support | 2020 |
| Eevo Training Ltd | Other services | 2022 |
| Efficore Limited | Professional & scientific | 2024 |
| Effinitiservices Ltd | Transport & logistics | 2025 |
| EHO Change Ltd | Professional & scientific | 2025 |
| Eikon Graphic Design Limited | Professional & scientific | 2003 |
| Eiredale Limited | Real estate | 2011 |
| EL Maintenance Limited | Other services | 2018 |
| EL One Holdings Limited | Real estate | 2024 |