| DTM Global Limited | Information & communication | 2010 |
| DTS Plant Ltd | Water & waste | 2024 |
| Duchy Brickwork Limited | Construction | 2019 |
| Dudley & Griffiths Ltd | Real estate | 2022 |
| Durnford Catering Ltd | Hospitality | 2025 |
| DW Signs Limited | Professional & scientific | 2023 |
| DWD Fabrications Limited | Manufacturing | 2005 |
| Dynamic Nation Limited | Administrative & support | 2014 |
| E.F. Hender & SON Limited | Agriculture & fishing | 1962 |
| Eco Cept Ltd | Wholesale & retail | 2013 |
| Eco NRG Ltd | Construction | 2009 |
| Eco-Heat (SW) Limited | Construction | 2010 |
| Ecoattire Ltd | Wholesale & retail | 2022 |
| Ecotec Construction Ltd | Construction | 2023 |
| Eden FOX Developments Ltd | Construction | 2025 |
| Edenic States Limited | Wholesale & retail | 2021 |
| EDS Autocore Ltd | Wholesale & retail | 2015 |
| Eggbuckland Chippy Ltd | Hospitality | 2018 |
| Eggbuckland Takeaway Ltd | Hospitality | 2024 |
| Eliveate Care & Support Limited | Health & social care | 2012 |
| Eljay Energy Ltd | Construction | 2001 |
| Elle Jones Ltd | Information & communication | 2020 |
| Ellerby-Jean Homes Ltd | Real estate | 2025 |
| Ellessy Dental Care Ltd | Health & social care | 2024 |
| Elliott & Partners (UK) Limited | Professional & scientific | 2002 |
| Ellis & Young Developments Ltd | Construction | 2025 |
| Ellis & Young Equities Ltd | Real estate | 2025 |
| Emotional Logic Centre | Education | 2003 |
| Emrock & Co. Ltd | Construction | 2001 |
| Enactiq Advisory Ltd | Information & communication | 2026 |
| Encore Build Ltd | Construction | 2024 |
| Energy & Power Tech Ltd | Construction | 2018 |
| Energy Build Southwest Ltd | Construction | 2023 |
| Energy Developments Southwest Ltd | Real estate | 2024 |
| Energy Group Southwest Ltd | Financial services | 2025 |
| Engineering Solutions (SW) Ltd | Professional & scientific | 2021 |
| Entity Flooring Ltd | Construction | 2023 |
| Era-Fit Ltd | Arts & recreation | 2015 |
| Erme Decorating Limited | Administrative & support | 2017 |
| Erme Lettings Ltd | Real estate | 2025 |
| Erme Property Ltd | Real estate | 2024 |
| Erme Valley Riding for the Disabled Ltd | Health & social care | 2011 |
| Erney Ltd. | Real estate | 2013 |
| Estate Fencing (SW) Ltd | Construction | 2020 |
| Esterlam International Limited | Manufacturing | 1984 |
| Ethicrecruit Limited | Administrative & support | 2017 |
| Euroservice Garage Ltd | Wholesale & retail | 2011 |
| Evered Stevens Maggi Fisher Limited | Wholesale & retail | 1978 |
| Evergreen Guiding Ltd | Education | 2026 |
| Exelets Ltd | Construction | 2021 |
| Exeter Carpentry & Construction Ltd. | Manufacturing | 2025 |
| Exeter Neuro Spine Limited | Health & social care | 2025 |
| External Facade Systems Ltd | Construction | 2011 |
| Extra Help Ltd | Health & social care | 2010 |
| Extreme Events Ltd | Arts & recreation | 2023 |
| F and J Collection Limited | Wholesale & retail | 2013 |
| Fair Forward Limited | Professional & scientific | 2025 |
| Fairford Electronics Holdings Limited | Financial services | 2018 |
| Fairford Electronics Limited | Manufacturing | 2018 |
| Fairline Yachts Holding Limited | Financial services | 2021 |
| Falcon Environmental Services Ltd | Agriculture & fishing | 2016 |
| Fallow Tattoo Collective Limited | Other services | 2025 |
| Faultless Finish Ltd | Construction | 2020 |
| Fawns Eco Gardening Services Ltd | Administrative & support | 2026 |
| Fawns Farm Limited | Agriculture & fishing | 2025 |
| Feathers Wharf Ltd | Extraterritorial | 1998 |
| Feloriker Fit Ltd | Arts & recreation | 2025 |
| FG Bespoke Ltd | Health & social care | 2024 |
| Field Services (Plymouth) Limited | Wholesale & retail | 2011 |
| Field Services Recovery Limited | Wholesale & retail | 2026 |
| Fifteen Eleven Ltd | Health & social care | 2021 |
| Filham Consultancy Ltd | Professional & scientific | 2026 |
| Finchia Pembs Ltd | Health & social care | 2019 |
| Findik Limited | Hospitality | 2023 |
| Fine Style Windows South West Ltd | Construction | 2019 |
| Fireproof Paint Limited | Professional & scientific | 2012 |
| First Steps Family Mediation Limited | Administrative & support | 2024 |
| First Sterling Investments Limited | Real estate | 2006 |
| Five OUT Limited | Financial services | 2025 |
| Flagstaff Defence Ltd | Public administration | 2026 |
| Floor Dynamics Limited | Professional & scientific | 2018 |
| Followall Ltd | Information & communication | 2023 |
| Food Infrastructure Accelerator Limited | Financial services | 2024 |
| Food Station Ltd | Hospitality | 2025 |
| Ford Consultants Ltd | Health & social care | 2012 |
| Ford Educational Psychology Services Ltd | Education | 2022 |
| Ford Lettings Ltd | Real estate | 2025 |
| Fowlescombe Farm LLP | | 2024 |
| FOX & Co Associates Ltd | Professional & scientific | 2019 |
| FOX Payroll Services Ltd | Professional & scientific | 2025 |
| Frasers Plumbing & Heating Solutions Ltd | Construction | 2024 |
| Freddie's Event Catering Ltd | Hospitality | 2026 |
| Freddie's Kitchen Ltd | Hospitality | 2024 |
| Fresh Fades Ltd | Other services | 2018 |
| Freydex Limited | Construction | 2024 |
| Full Spectrum Design and Consultancy Ltd | Professional & scientific | 2024 |
| Furze-Painter Driving School Ltd | Education | 2025 |
| Furzeland Estates Limited | Wholesale & retail | 1935 |
| Furzeland Holdings Ltd | Professional & scientific | 2016 |
| G&D Motor Repairs Ltd | Wholesale & retail | 2025 |