| 103 Portland Ltd | Financial services | 2014 |
| 1STATWIND Ltd | Manufacturing | 2025 |
| 22 Zero Eight Ltd | Administrative & support | 2012 |
| 360 Project Controls Limited | Construction | 2026 |
| 4 Square Property Ltd. | Real estate | 2009 |
| 5TH Wheel Accident Claims Limited | Financial services | 2019 |
| A & D Properties (Ayrshire) Ltd | Real estate | 2014 |
| A and D Transport Limited | Transport & logistics | 2025 |
| A BEE Place Limited | Hospitality | 2026 |
| A Chilton Ltd | Administrative & support | 2016 |
| A G Motors (Ayrshire) Ltd | Wholesale & retail | 2024 |
| A H W Lygate Epcs Ltd | Construction | 2026 |
| A K Christie Ltd | Professional & scientific | 2013 |
| A SIM Decor Limited | Construction | 2008 |
| A&A Motorcycles (North Harbour) Ltd | Wholesale & retail | 2026 |
| A&H Security Services Limited | Administrative & support | 2025 |
| A-Mc Pipeshop Engineering Ltd | Professional & scientific | 2026 |
| A.G.B Industrial Ltd | Professional & scientific | 2013 |
| A.S.C.(Ayrshire SAW Centre) Ltd | Manufacturing | 2020 |
| A.Vogel Limited | Wholesale & retail | 1986 |
| A1MB Ltd | Administrative & support | 2026 |
| AA Optical Ltd | Wholesale & retail | 2014 |
| AB1 Retail Ltd | Real estate | 2020 |
| Abandoned Storage Lockers Limited | Wholesale & retail | 2020 |
| Abbi Lets Ltd | Real estate | 2013 |
| ABC Accommodation Ltd. | Real estate | 2001 |
| Abhar Barbers Ltd | Other services | 2016 |
| AC Facility Solutions Limited | Construction | 2015 |
| ACS Cars Ltd | Transport & logistics | 2025 |
| ACS Energy Ltd | Administrative & support | 2021 |
| Activon Limited | Professional & scientific | 2019 |
| Activon Property Management Limited | Real estate | 2022 |
| Adam and Co Accountants (Ayrshire) Ltd | Professional & scientific | 2018 |
| ADD One (Scotland) Limited | Professional & scientific | 2000 |
| ADM Properties (Scotland) Ltd | Real estate | 2020 |
| Adonis Signalling Ltd | Transport & logistics | 2013 |
| Adzahm Properties Ltd | Real estate | 2022 |
| Aero Consulting Engineering Products Ltd | Wholesale & retail | 2023 |
| Aero Consulting Engineers Ltd | Administrative & support | 2017 |
| Aesthetic Physiques Ltd | Wholesale & retail | 2015 |
| Aesthetics by a Ltd | Health & social care | 2023 |
| AFS Financial Partners Ltd | Financial services | 2024 |
| Agnew Properties Limited | Real estate | 2025 |
| Ailsa Management Services Limited | Professional & scientific | 1984 |
| Airdrone Ltd | Manufacturing | 2020 |
| Aire&Co Ltd | Hospitality | 2021 |
| Aitken Roofing & Roughcasters Ltd | Construction | 2024 |
| Aitken Roofing Ltd. | Construction | 2008 |
| Aivora Finance Limited | Information & communication | 2026 |
| AJM Costings Limited | Professional & scientific | 2016 |
| AKA Connections Ltd | Administrative & support | 2024 |
| Al's Sign Shack Ltd | Professional & scientific | 2024 |
| Albann Irvine Limited | Extraterritorial | 2000 |
| Albann Limited | Manufacturing | 2006 |
| Albion Douglas Limited | Hospitality | 1997 |
| Alerta Medical Ltd | Manufacturing | 2019 |
| Alex Mcmaster Builders Ltd | Construction | 2021 |
| ALH Group Limited | Professional & scientific | 2008 |
| Alisha-Package Dash Ltd | Transport & logistics | 2025 |
| Allt Solutions Ltd | Manufacturing | 2018 |
| Almswall Ltd | Wholesale & retail | 2018 |
| Ambio Solutions Ltd | Professional & scientific | 2019 |
| Anahar Limited | Manufacturing | 2026 |
| Anderson Bakx Ltd | Agriculture & fishing | 2022 |
| Andersonic Ltd | Information & communication | 2020 |
| Andrew Byers Project Controls Ltd | Construction | 2019 |
| Andrew Wright Group Holdings Limited | Financial services | 2025 |
| Andrew Wright Limited | Manufacturing | 2025 |
| Andrew Wright Trading Limited | Construction | 2025 |
| Anne's Store Ltd | Wholesale & retail | 2025 |
| Annefield Supplies Limited | Other services | 2001 |
| Annfield House | | 1998 |
| Annick Bathrooms Ltd | Construction | 2025 |
| Annick Group Ltd | Construction | 2023 |
| Aortech International Limited | Extraterritorial | 2020 |
| AP Musclefit Ltd | Wholesale & retail | 2017 |
| Apperley Aviation Services Ltd | Manufacturing | 2026 |
| Aqua Sharks Ltd | Education | 2022 |
| Arad Barber Ltd | Other services | 2026 |
| Archtur Group Ltd | Real estate | 2025 |
| Arena Office Limited | Wholesale & retail | 2012 |
| Arian&Co Limited | Hospitality | 2024 |
| Arrontec Ltd | Information & communication | 2022 |
| Arrt Properties Ltd | Real estate | 2024 |
| AS Access Ltd | Construction | 2025 |
| ASC Asset Management (EK) Ltd | Real estate | 2011 |
| Ascension Business Advisers Ltd | Professional & scientific | 2023 |
| ASF Khan Limited | Wholesale & retail | 2022 |
| Ashe Holdings Ltd | Transport & logistics | 2018 |
| Ashtech Inspection Ltd. | Construction | 2008 |
| Aspect Land and Hydrographic Surveys Limited | Professional & scientific | 2009 |
| Aspect Surveys Limited | Financial services | 2009 |
| Aspiration Accounts Ltd | Professional & scientific | 2023 |
| Aspire Minds Ltd | Health & social care | 2022 |
| Asset Integrity (Ayrshire) Ltd | Professional & scientific | 2019 |
| ATS (Scotland) Ltd | Transport & logistics | 2024 |
| Attic Events Ltd | Arts & recreation | 2017 |
| Auchengate Carriage Driving Club Ltd | Agriculture & fishing | 2021 |
| Auchengate Pallet Manufacturer Ltd | Manufacturing | 2025 |
| Auchenmade Quarry Limited | Other services | 2016 |