| Aurahigh Ltd | Agriculture & fishing | 2025 |
| Automotive Passion Scotland Ltd | Wholesale & retail | 2019 |
| Aviation, Power & Marine Limited | Manufacturing | 2015 |
| Avitec Engineering Limited | Manufacturing | 2017 |
| AWD Access Solutions Ltd | Construction | 2025 |
| AWD Environmental Ltd | Professional & scientific | 2023 |
| Axiom IMD Limited | Wholesale & retail | 2018 |
| AY Investments Ltd | Real estate | 2022 |
| AY Properties 1 Ltd | Real estate | 2019 |
| Ayrshire Blinds Limited | Manufacturing | 1984 |
| Ayrshire Garden Services Ltd | Manufacturing | 2026 |
| Ayrshire Gasworks Ltd | Construction | 2013 |
| Ayrshire Gifts Ltd | Wholesale & retail | 2024 |
| Ayrshire Insulation Services Ltd | Construction | 2024 |
| Ayrshire IT Ltd | Information & communication | 2018 |
| Ayrshire MOT Ltd | Wholesale & retail | 2023 |
| Ayrshire Pallets & Timber Products Ltd | Other services | 2023 |
| Ayrshire Proclean Limited | Administrative & support | 2025 |
| Ayrshire Properties Ltd | Real estate | 2025 |
| Ayrshire Property Management Ltd. | Real estate | 2010 |
| Ayrshire Safety Netting Services Limited | Construction | 2020 |
| Ayrshire Software Solutions Ltd | Information & communication | 2016 |
| Ayrshire Swim Hub Ltd | Arts & recreation | 2025 |
| Ayrshire Trailers Ltd | Wholesale & retail | 2024 |
| Ayrshire Valeting Centre Ltd | Administrative & support | 2026 |
| B Fairbairn Contract Management Consultant | | 1997 |
| Baking With Attitude Ltd | Wholesale & retail | 2022 |
| Balmoral Funerals Ltd | Other services | 2026 |
| Barlow Engineering Services Limited | Professional & scientific | 2017 |
| Barnhill Boxes Ltd | Wholesale & retail | 2020 |
| Barony International Limited | Wholesale & retail | 2011 |
| Barr Transport Scotland Ltd | Transport & logistics | 2023 |
| BAS Trading Scot Limited | Wholesale & retail | 2023 |
| Baseline JIU Jitsu Limited | Arts & recreation | 2023 |
| Basix Gym Limited | Arts & recreation | 2008 |
| BB Taverns Limited | Hospitality | 2023 |
| BE Property Holdings Ltd | Real estate | 2020 |
| BE Property Mentors Ltd | Households | 2019 |
| Beach Club Tanning Ltd | Wholesale & retail | 2025 |
| Beijing Chef 88 Limited | Hospitality | 2021 |
| Believe Dance Competition Ltd | Arts & recreation | 2024 |
| Bell Maintenance Limited | Construction | 2013 |
| Bevpac Engineering Consultants Ltd | Professional & scientific | 2025 |
| BGM Comms Ltd | Information & communication | 2026 |
| BH Roofing (Ayrshire) Limited | Construction | 2021 |
| Biknav Properties Ltd | Real estate | 2021 |
| Birring Stores (Galston) Ltd | Wholesale & retail | 2026 |
| Birring Stores (Kilmarnock) Ltd | Wholesale & retail | 2019 |
| Bizmogul Ltd | Construction | 2025 |
| BK Eatery Ltd | Hospitality | 2023 |
| BKU Construction Limited | Construction | 2015 |
| BKU Homes Ltd | Real estate | 2020 |
| BKU Store Ltd | Wholesale & retail | 2023 |
| Black Cat Haulage Ltd | Transport & logistics | 2011 |
| Blades of Glory Barber Club Ltd | Other services | 2026 |
| Blair Installations Limited | Construction | 2025 |
| Blair's Kitchens Bathrooms and Bedrooms Ltd | Construction | 2023 |
| Blairs Catering Equipment Limited | Manufacturing | 2013 |
| Blairs Limited | Financial services | 2025 |
| Blindco Ayrshire Limited | Construction | 2025 |
| Bloom Marketing Solutions Ltd | Professional & scientific | 2024 |
| Boat & Building Cleaning Solutions Ltd | Administrative & support | 2021 |
| Boat Electrics & Electronics (Holdings) Ltd | Financial services | 2015 |
| Boat Electrics & Electronics Limited | Manufacturing | 1978 |
| Bobby Khan Enterprises Ltd | Wholesale & retail | 2010 |
| Bobby,S Joinery Products Limited | Manufacturing | 2015 |
| Booth Welsh Nexus Limited | Professional & scientific | 2023 |
| Boss Salon Ltd | Wholesale & retail | 2024 |
| Braidwood Carpets & Flooring Ltd | Wholesale & retail | 2013 |
| Brakemaster Automotive Technical Services Ltd | Professional & scientific | 2025 |
| Bright Star Glasgow Ltd | Other services | 2019 |
| Broomlands Group Ltd | Wholesale & retail | 2022 |
| Brotherton Diving Limited | Manufacturing | 2024 |
| Buchanan Plastering Roughcasting Ltd | Construction | 2025 |
| Burnswark (Scotland) Limited | Professional & scientific | 2023 |
| Buy Your Property Ltd | Real estate | 2022 |
| BWA (Holdings) Limited | Financial services | 2011 |
| BWA Nexus Limited | Construction | 2000 |
| BWG Transport Limited | Transport & logistics | 2019 |
| C & D Leisure Ltd. | Hospitality | 2008 |
| C & H Craig Ltd | Wholesale & retail | 2019 |
| C Brown Décor Ltd | Construction | 2017 |
| C.C.S. Engineering Company Limited | Manufacturing | 1974 |
| C.M.D. Performance Limited | Transport & logistics | 2018 |
| C.V. Labels Limited | Manufacturing | 1989 |
| Cabello by Caroline Ltd | Other services | 2021 |
| Calbost IT Limited | Information & communication | 2014 |
| Calidus Ltd | Construction | 2025 |
| Calistri Hotels (Holdings) Limited | Hospitality | 2010 |
| Capital Connect Ltd | Administrative & support | 2026 |
| Capital Cross Global Ltd | Wholesale & retail | 2014 |
| Captain Designs Limited | Administrative & support | 2018 |
| Care Springside Limited | Wholesale & retail | 2020 |
| Carly's Ltd | Other services | 2025 |
| Carr Veitch Ltd | Manufacturing | 2025 |
| Carrie on Solutions Ltd | Information & communication | 2025 |
| Cascade (Scotland) Limited | Other services | 2012 |
| Castle Groundworks Ltd | Other services | 2017 |
| Castrum Property and Sourcing Ltd | Real estate | 2025 |
| CC Property Developments Ltd | Construction | 2024 |