| Ermine Street Ltd | Construction | 2025 |
| Ermine Street Management Limited | Households | 1995 |
| ESC Automotive Ltd | Wholesale & retail | 2025 |
| ESE Lounge Limited | Hospitality | 2025 |
| Eshir Limited | Other services | 2007 |
| Essential Supermarket Ltd | Wholesale & retail | 2013 |
| Etamsi Ltd | Real estate | 2017 |
| Etask Solutions UK Ltd | Health & social care | 2026 |
| Eternal Construction (Cambridge) Ltd | Construction | 2025 |
| Ethnic Origins Limited | Wholesale & retail | 2001 |
| Etmp Consultancy Ltd | Professional & scientific | 2020 |
| Eunice Artisan Bakery Ltd | Manufacturing | 2009 |
| Eunice Properties Ltd | Real estate | 2021 |
| Euro A.C.C. Transport Ltd | Administrative & support | 2016 |
| Euro Shop (Huntingdon) Ltd | Wholesale & retail | 2021 |
| EVA Lettings Limited | Real estate | 2021 |
| EVE Property Management Limited | Professional & scientific | 2010 |
| Evecrest Limited | Wholesale & retail | 2023 |
| Evelyn Consultants Limited | Professional & scientific | 2022 |
| Evelyn Glennie Productions Limited | Administrative & support | 2009 |
| Everdell Landscapes Limited | Administrative & support | 2025 |
| Evergreen Property Services (Cambs) Ltd | Administrative & support | 2009 |
| Every Bite Nutrition Ltd | Health & social care | 2022 |
| Evesolution Ltd | Public administration | 2024 |
| Evolution Cricket Ltd | Arts & recreation | 2019 |
| Evolutiondome Limited | Arts & recreation | 2010 |
| Evolve 4 Group Limited | Financial services | 2020 |
| Evolve 4 Limited | Information & communication | 2020 |
| Evolve 4 Solutions Limited | Information & communication | 2020 |
| Evolve Staffing Solutions Ltd | Education | 2020 |
| Ewing Associates Limited | Financial services | 2006 |
| EX Health Well - Exercise Health & Wellbeing Ltd | Professional & scientific | 2025 |
| Exact Controls Ltd | Professional & scientific | 2020 |
| Exact System Solutions Limited | Construction | 2017 |
| Exacta Education Limited | Administrative & support | 2018 |
| Excalibur Films Limited | Information & communication | 2004 |
| Excelis Limited | Manufacturing | 2014 |
| Excite Global Media Ltd | Information & communication | 2017 |
| Exclusive Modern Bathroom Limited | Construction | 2024 |
| Experience Cambridge CIC | Administrative & support | 2024 |
| Expert MIS Ltd | Manufacturing | 2014 |
| Expert Training Ltd | Construction | 2021 |
| Expertrads Ltd | Health & social care | 2020 |
| Explorer-It (UK) Ltd | Information & communication | 2008 |
| Exploring Anything Together Ltd | Health & social care | 2016 |
| Exportability Limited | Professional & scientific | 2001 |
| Express Cars Private Hire Ltd | Transport & logistics | 2020 |
| Express Cartons Ltd | Wholesale & retail | 2013 |
| Exquisite Systems Ltd | Information & communication | 2008 |
| Extended Healthcare Limited | Health & social care | 2023 |
| Eyelink Limited | Extraterritorial | 1989 |
| Eyles Electrical Ltd | Construction | 2025 |
| Ezee Cleaning Services Ltd | Administrative & support | 2022 |
| Ezee Group Ltd | Administrative & support | 2025 |
| Ezee Services Ltd | Administrative & support | 2026 |
| F & S Taxis Ltd | Transport & logistics | 2020 |
| F&S Freelance Design Draughting Ltd | Information & communication | 2016 |
| F. Vindis & Sons (Bedford) Limited | Wholesale & retail | 1994 |
| F. Vindis & Sons (Peterborough) Limited | Wholesale & retail | 2010 |
| F. Vindis & Sons (Sawston) Limited | Wholesale & retail | 1984 |
| F. Vindis & Sons (ST. Ives) Limited | Wholesale & retail | 1982 |
| F.J.Roe Limited | Agriculture & fishing | 1957 |
| F17 Apparel Limited | Wholesale & retail | 2017 |
| Fabbash Properties Limited | Real estate | 2017 |
| FACE2FACEHR Partners Limited | Administrative & support | 2013 |
| Factory Tile Depot Limited | Extraterritorial | 2021 |
| Fadzieys Exquisite Catering Limited | Hospitality | 2021 |
| Fairley Properties Ltd | Real estate | 2021 |
| Fairview Medical Ltd | Health & social care | 2025 |
| Fairway (Bluntisham) Management Company Limited(The) | Households | 1983 |
| Fairway Importers Limited | Wholesale & retail | 1992 |
| FAIRWAY360 Limited | Professional & scientific | 2025 |
| Fairytale Ballet Limited | Arts & recreation | 2023 |
| Fairywood Property Management Ltd | Households | 2020 |
| Faith Care Line Services Limited | Administrative & support | 2017 |
| Falcon XL Ltd | Information & communication | 2015 |
| Falex Transport Services Ltd | Transport & logistics | 2022 |
| Falkotrans Ltd | Transport & logistics | 2019 |
| Fall River Holdings LLP | | 2016 |
| Fall X Safety Services Ltd | Construction | 2023 |
| Fallen Eden Limited | Professional & scientific | 2024 |
| Family Psychology Mutual CIC | Health & social care | 2015 |
| Farmland Reserve UK Limited | Real estate | 1977 |
| Fastnfar Ltd | Transport & logistics | 2025 |
| Father and SON Transport BG Limited | Transport & logistics | 2012 |
| Favourite Tables Limited | Hospitality | 2014 |
| FD Partners Recruitment Ltd | Administrative & support | 2024 |
| FDM Pet Grooming Limited | Other services | 2022 |
| Feather Nest Farms Ltd | Agriculture & fishing | 2025 |
| Fedeson Limited | Health & social care | 2014 |
| FEN Cowboys Ltd | Wholesale & retail | 2025 |
| FEN Lane Holdings Limited | Financial services | 2025 |
| FEN Property Service Ltd | Construction | 2023 |
| FEN View Pet Clinic Limited | Professional & scientific | 2025 |
| Fengate Land Holdings Limited | Construction | 2021 |
| Fenland Best Interest Assessors Ltd | Health & social care | 2024 |
| Fenland District Brokers Limited | Financial services | 2022 |
| Fenland Ebikes Ltd | Wholesale & retail | 2019 |
| Fenland Equestrian Services Ltd | Agriculture & fishing | 2015 |
| Fenland Plant Glazing Ltd | Wholesale & retail | 2014 |