| SH60 Music Consultancy Limited | Arts & recreation | 2022 |
| Shane Francis Flooring Limited | Construction | 2018 |
| Shannon White Ltd | Administrative & support | 1997 |
| Shantisoft Limited | Information & communication | 1997 |
| Shapeshift Ventures Ltd | Financial services | 2025 |
| Shardygale Ltd | Professional & scientific | 2015 |
| Share Retreats Limited | Administrative & support | 2025 |
| Shared Enterprise CIC | Education | 2014 |
| Shariff's Properties Ltd | Real estate | 2024 |
| Sharon Quinn Business Psychology Ltd | Professional & scientific | 2026 |
| Sharp Edge Blades Ltd | Manufacturing | 2024 |
| Shaw Design Services Limited | Professional & scientific | 2000 |
| SHC Projects Limited | Other services | 1998 |
| Shed Maria Bonita Ltd | Arts & recreation | 2023 |
| Sheikh Sons Limited | Mining & quarrying | 2025 |
| Shelley News Holdings Ltd | Financial services | 2019 |
| Shelley News Ltd | Wholesale & retail | 2011 |
| Shepheard Accounting Limited | Professional & scientific | 2012 |
| Shepherd's Care Ltd | Households | 2023 |
| Sheridan Farming LLP | | 2019 |
| Shermanbury Grange Land Management Company Limited | Agriculture & fishing | 2018 |
| Shermanbury Grange Management Company Limited | Households | 2001 |
| Shermanbury Stone Clad Apartments Limited | Households | 2015 |
| Shielporter Communications Limited | Professional & scientific | 2011 |
| Shift IT Same Day Delivery Ltd | Transport & logistics | 2023 |
| Shimtim Concepts Limited | Construction | 2017 |
| Shindigs Events Limited | Other services | 2016 |
| Shine for Life CIC | Health & social care | 2025 |
| Shine for Life Equine Assisted Therapy C.I.C. | Health & social care | 2012 |
| Shineydogdesigns Ltd | Professional & scientific | 2024 |
| Shiny Glow Ltd | Other services | 2023 |
| Shoare Bloodstock Ltd | Agriculture & fishing | 2024 |
| Shopper Measures (UK) Limited | Professional & scientific | 2017 |
| Shorn Ltd | Agriculture & fishing | 2023 |
| Showgreen Limited | Hospitality | 1992 |
| Shuyona Holding Company Limited | Financial services | 2020 |
| Shwari Group Ltd | Wholesale & retail | 2025 |
| Shyft Studio CIC | Education | 2025 |
| Siberian Resources Limited | Mining & quarrying | 2014 |
| Siena Homes Ltd | Construction | 2025 |
| Sienna Jones Limited | Wholesale & retail | 2015 |
| Sienna Property Limited | Real estate | 2019 |
| Sienna Rocks Limited | Wholesale & retail | 2014 |
| Sight Loss Spectrum Ltd | Wholesale & retail | 2026 |
| Sigicom Limited | Administrative & support | 2017 |
| Sigma Projects and Maintenance Ltd | Construction | 2025 |
| Signage, Events & More Ltd | Manufacturing | 2024 |
| Signcomp Europe Limited | Wholesale & retail | 1998 |
| Signcomp Holdings Limited | Financial services | 2016 |
| Silicon Flight Limited | Information & communication | 2010 |
| Silva Oak Construction Ltd | Construction | 2023 |
| Silver Lifestyle Limited | Information & communication | 2017 |
| Silver Media and Events Limited | Information & communication | 2023 |
| Silver Quad Limited | Real estate | 2015 |
| Silver Wings Healthcare Ltd | Health & social care | 2025 |
| Silverback Studios Limited | Professional & scientific | 2012 |
| Silvertip Films Limited | Information & communication | 2007 |
| Silvertree Healthcare Limited | Professional & scientific | 2010 |
| Silverwave Equity Partners Ltd | Professional & scientific | 2024 |
| Simenza Importazioni Ltd | Wholesale & retail | 2023 |
| Simon Connell Property Advisory Limited | Real estate | 2026 |
| Simon Edwards (Utilities) Consulting Ltd | Professional & scientific | 2017 |
| Simon Jordan Limited | Wholesale & retail | 2024 |
| Simple Garden Rooms Limited | Wholesale & retail | 2020 |
| Simplecubed Limited | Other services | 2014 |
| Simply Horsing Around Ltd | Agriculture & fishing | 2023 |
| Simply Sorted Limited | Manufacturing | 2015 |
| Simply Topps Ltd | Hospitality | 2012 |
| Simprep Airline Training Limited | Education | 2024 |
| Sinclair Barnes Limited | Professional & scientific | 2002 |
| Sindy Bond Limited | Other services | 2015 |
| Singh Builders West Sussex Ltd | Construction | 2022 |
| Sinnott Green Ltd | Real estate | 2003 |
| SIP & Slide Ltd | Hospitality | 2024 |
| SIR Francis Pittis & SON Limited | Administrative & support | 1989 |
| Siric Communications Limited | Information & communication | 2012 |
| Siso Agency Ltd | Professional & scientific | 2019 |
| Site Safety Management Services Ltd | Professional & scientific | 2017 |
| Siw-Uk Ltd | Wholesale & retail | 2023 |
| SJB Cost Consulting Ltd | Professional & scientific | 2024 |
| SJE Management Ltd | Professional & scientific | 2012 |
| SJL24 Ltd | Arts & recreation | 2026 |
| SJM Training Ltd | Professional & scientific | 2024 |
| Sjte Commercial Limited | Professional & scientific | 2017 |
| SK Construction Consultancy Limited | Professional & scientific | 2015 |
| Ski-Mojo Limited | Manufacturing | 2020 |
| Skiandsnowboard Limited | Wholesale & retail | 2017 |
| Skill International Ltd | Wholesale & retail | 2020 |
| Skin Beautiful Ltd | Other services | 2012 |
| Skingenius Skincare Limited | Other services | 2015 |
| Skintek Limited | Health & social care | 2009 |
| Skipster Limited | Professional & scientific | 2010 |
| Skn-Ray Ltd | Other services | 2026 |
| Skycloud Technologies Limited | Information & communication | 2020 |
| Skyland Builders Limited | Wholesale & retail | 2008 |
| Skylark Global Ltd | Professional & scientific | 2023 |
| Skytrail Limited | Administrative & support | 1983 |
| Skyways Transport Services Limited | Transport & logistics | 1998 |
| Slap Burger Limited | Hospitality | 2024 |
| SLC Beauty Ltd | Other services | 2026 |