| 1530 Ltd | Hospitality | 2010 |
| 200 Acres Ltd | Construction | 2024 |
| 4D Therapy Ltd | Health & social care | 2019 |
| A and a Graham Limited | Information & communication | 2019 |
| A Hulbert Ltd | Administrative & support | 2021 |
| A M Smith Construction Limited | Administrative & support | 2009 |
| A1 Agency Limited | Arts & recreation | 1998 |
| Accessrite Limited | Construction | 2020 |
| Acoustic Blue (Music Productions) Limited | Arts & recreation | 1990 |
| Advantage Book Company Limited | Real estate | 1970 |
| Aerofix Paragliding Services Limited | Other services | 2015 |
| AJ Paws Limited | Professional & scientific | 2021 |
| AJC CF Limited | Professional & scientific | 2023 |
| AJC Corporate Limited | Professional & scientific | 2026 |
| AK Web Solutions Limited | Information & communication | 2012 |
| ALL Aboard Nursery Ltd | Health & social care | 2016 |
| Alphaweld Limited | Manufacturing | 1992 |
| Altavis Limited | Administrative & support | 2025 |
| Altura Safety and Training Limited | Professional & scientific | 2014 |
| Andrew Stuart & Company Limited | Financial services | 2002 |
| Andy Cave Ltd | Arts & recreation | 2014 |
| Anglers Rest (Bamford) Limited | Hospitality | 2025 |
| Anvil Business Advisory Limited | Administrative & support | 2016 |
| Anyauto Limited | Wholesale & retail | 2025 |
| Apex Plumbing and Heating Services Limited | Construction | 2011 |
| Appscore Limited | Professional & scientific | 2018 |
| Aquagate Ltd | Administrative & support | 2012 |
| Arbsites Limited | Information & communication | 2010 |
| Architect Studio Gedye Limited | Professional & scientific | 2015 |
| Arete Tutoring Limited | Education | 2022 |
| Arhkel Limited | Professional & scientific | 2023 |
| ARP Brightmore Limited | Professional & scientific | 2004 |
| Artled Productions CIC | Administrative & support | 2024 |
| Assured Social Care Ltd | Health & social care | 2019 |
| Aston Hall Ltd | Hospitality | 2025 |
| At&C (Asbestos Training & Consultancy) Limited | Education | 2009 |
| Aussie Tree Care Limited | Administrative & support | 2024 |
| Automated Bioacoustics Ltd | Professional & scientific | 2022 |
| Avian Limited | Manufacturing | 1989 |
| Avill Construction Ltd | Construction | 2024 |
| AWG Access Ltd | Construction | 2013 |
| B & K Percival (Plumbers) Limited | Other services | 1993 |
| B & W Auto Care Limited | Other services | 2007 |
| Bakewell Cottages Limited | Hospitality | 2017 |
| Ballington Brothers Limited | Administrative & support | 1979 |
| Bamford Consulting, Contracting, and Coaching Limited | Professional & scientific | 2018 |
| Bamford Mill Residents Limited | Households | 1997 |
| Bancroft Builders Limited | Construction | 2012 |
| Band of Birds Limited | Professional & scientific | 2014 |
| Barbrook Developments Limited | Real estate | 2019 |
| Base Camp Hathersage Ltd | Hospitality | 2024 |
| Basic Skills Solutions Limited | Professional & scientific | 2006 |
| Baslow Edge Nursery Limited | Health & social care | 2017 |
| Baslow Properties Limited | Real estate | 2020 |
| BCB Ecommerce Ltd | Wholesale & retail | 2022 |
| BDI Medical Limited | Health & social care | 2012 |
| Bedford and Havenhand Shopfitting Ltd | Manufacturing | 2024 |
| BEE Safe Supplies Ltd | Manufacturing | 2020 |
| Beech Hurst Ltd | Hospitality | 2024 |
| Belphegor Investments Ltd | Real estate | 2026 |
| BEN Chell Consultancy Ltd | Health & social care | 2016 |
| Benchmark (Bakewell) Ltd. | Construction | 2004 |
| Beyond Excellence Healthcare Consultancy Limited | Professional & scientific | 2020 |
| Big Moor Limited | Professional & scientific | 2023 |
| Big Stone Limited | Other services | 2002 |
| Birchfield Hall Management Company Limited | Households | 2000 |
| Birley View Management Company Limited | Households | 2002 |
| Blue John Cavern Limited | Arts & recreation | 2024 |
| Blue John Gems Limited | Wholesale & retail | 2012 |
| Bluestar Environmental Services Limited | Water & waste | 2002 |
| Bolton Gray Ltd. | Extraterritorial | 1985 |
| Borderquarry Limited | Professional & scientific | 1982 |
| Boulevard Music Ltd. | Arts & recreation | 2014 |
| BPS Land Holdings Limited | Real estate | 2020 |
| Bradda Dads CIC | Arts & recreation | 2018 |
| Bradwell Community Orchard Group | Agriculture & fishing | 2010 |
| Bradwell Springs Management Company Ltd | Households | 2020 |
| Brickfive Limited | Real estate | 2013 |
| Brookside Consulting Limited | Information & communication | 2013 |
| Broomhill Bouldering Company Ltd | Arts & recreation | 2025 |
| Brosterfield Caravan Park Limited | Hospitality | 2023 |
| Brosterfield Holiday Lets Ltd | Hospitality | 2020 |
| Brosterfield Investments Ltd | Financial services | 2016 |
| Buxoplas Manufacturing Limited | Manufacturing | 2014 |
| Buxoplas Pipe Systems Group Limited | Financial services | 2022 |
| Buxoplas Pipe Systems Limited | Manufacturing | 2011 |
| Cafe 19 at White's Limited | Hospitality | 2022 |
| Call Law Limited | Administrative & support | 2020 |
| Calver Arms Limited | Hospitality | 2023 |
| Calver Group Limited | Financial services | 2020 |
| Calver Peak Consulting Limited | Professional & scientific | 2023 |
| Calver Weir Restoration Project | Administrative & support | 2004 |
| Cambion Electronics Limited | Manufacturing | 1961 |
| Camperstay Limited | Information & communication | 2026 |
| Canine Caddies Limited | Other services | 2025 |
| Carbolite Gero Limited | Manufacturing | 1978 |
| Career Therapy Limited | Administrative & support | 2019 |
| Caudata Ltd | Information & communication | 2010 |
| Cavendish Engineering Services Limited | Manufacturing | 2002 |
| CDR Group Limited | Information & communication | 2006 |